protein coding gene - ssb1 (SPBC660.13c) - DNA replication factor A subunit Ssb1

Gene summary

Standard name

ssb1

Systematic ID

SPBC660.13c

Product

DNA replication factor A subunit Ssb1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

rpa1, rad11, rpa70

UniProt ID

Q92372

ORFeome ID

46/46B02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 219182..221298 reverse strand

Annotation

Protein features

Orthologs

• RPA1 (HGNC:10289) Homo sapiens
• ssb1 (SJAG_03855) Schizosaccharomyces japonicus
• RFA1 (YAR007C) Saccharomyces cerevisiae

References / Literature

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:166019 - Genetic control of radiation sensitivity in Schizosaccharomyces pombe.

Nasim A et al. Genetics 1975 Apr;79(4):573-82

PMID:34228709 - Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.

Soriano I et al. PLoS Genet 2021 Jul;17(7):e1009526

PMID:29215009 - The end-joining factor Ku acts in the end-resection of double strand break-free arrested replication forks.

Teixeira-Silva A et al. Nat Commun 2017 Dec 07;8(1):1982

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:41729970 - The RPA-binding domain and the KKRK motif in Rad26ATRIP cooperate at the perturbed DNA replication fork for initiating checkpoint signalling.

Xu YJ et al. PLoS Genet 2026 Feb 23;22(2):e1012052

PMID:22354040 - RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.

Luciano P et al. EMBO J 2012 Apr 18;31(8):2034-46

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

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PMID:10512870 - A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.

Suto K et al. Mol Biol Cell 1999 Oct;10(10):3331-43

PMID:28334931 - Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast.

Manjón E et al. Nucleic Acids Res 2017 May 19;45(9):5269-5284

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:17936710 - Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.

Limbo O et al. Mol Cell 2007 Oct 12;28(1):134-46

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:11600706 - SUMO modification of Rad22, the Schizosaccharomyces pombe homologue of the recombination protein Rad52.

Ho JC et al. Nucleic Acids Res 2001 Oct 15;29(20):4179-86

PMID:31563844 - The roles of fission yeast exonuclease 5 in nuclear and mitochondrial genome stability.

Sparks JL et al. DNA Repair (Amst) 2019 Nov;83:102720

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.

Zeng M et al. Nat Commun 2016 Apr 21;7:11364

PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.

Escandell JM et al. EMBO J 2019 Apr 01;38(7)

PMID:19158664 - Smc5/6 maintains stalled replication forks in a recombination-competent conformation.

Irmisch A et al. EMBO J 2009 Jan 21;28(2):144-55

PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.

Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58

PMID:24186976 - Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition.

Murayama Y et al. Genes Dev 2013 Nov 01;27(21):2299-304

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.

Larochelle M et al. J Cell Sci 2019 May 31;132(11)

PMID:37200372 - Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast.

Xu YJ et al. PLoS Genet 2023 May;19(5):e1010691

PMID:11560889 - Multiple interactions among the components of the recombinational DNA repair system in Schizosaccharomyces pombe.

Tsutsui Y et al. Genetics 2001 Sep;159(1):91-105

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:17429064 - Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss.

Kibe T et al. Mol Biol Cell 2007 Jun;18(6):2378-87

PMID:29358048 - Establishment of DNA-DNA Interactions by the Cohesin Ring.

Murayama Y et al. Cell 2018 Jan 25;172(3):465-477.e15

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27729451 - Cdc24 Is Essential for Long-range End Resection in the Repair of Double-stranded DNA Breaks.

Zhang H et al. J Biol Chem 2016 Nov 25;291(48):24961-24973

PMID:32541066 - RecQ DNA Helicase Rqh1 Promotes Rad3 ATR Kinase Signaling in the DNA Replication Checkpoint Pathway of Fission Yeast.

Ahamad N et al. Mol Cell Biol 2020 Aug 14;40(17)

PMID:14654689 - A novel allele of fission yeast rad11 that causes defects in DNA repair and telomere length regulation.

Ono Y et al. Nucleic Acids Res 2003 Dec 15;31(24):7141-9

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:20140190 - A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.

Subramanian L et al. PLoS Genet 2010 Feb 05;6(2):e1000839

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

PMID:40594858 - Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.

Bakosova A et al. Sci Rep 2025 Jul 01;15(1):21895

PMID:9658208 - Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants.

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PMID:28806726 - Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA.

Iyer DR et al. PLoS Genet 2017 Aug;13(8):e1006958

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:8702843 - Purification, gene cloning, and reconstitution of the heterotrimeric single-stranded DNA-binding protein from Schizosaccharomyces pombe.

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PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.

Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:23211746 - Initiation of DNA damage responses through XPG-related nucleases.

Kuntz K et al. EMBO J 2013 Jan 23;32(2):290-302

PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.

Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:21931565 - Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks.

Langerak P et al. PLoS Genet 2011 Sep;7(9):e1002271

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:23779158 - The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.

Saito Y et al. Sci Rep 2013;3:2022

PMID:31260531 - Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.

González-Medina A et al. Nucleic Acids Res 2019 Sep 19;47(16):8439-8451

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.

Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.

Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626

PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.

Matmati S et al. Sci Adv 2018 May;4(5):eaar2740

PMID:9111307 - The Schizosaccharomyces pombe rad11+ gene encodes the large subunit of replication protein A.

Parker AE et al. Mol Cell Biol 1997 May;17(5):2381-90