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protein coding gene - orc2 (SPBC685.09) - origin recognition complex subunit Orc2

Gene summary

Standard name
orc2
Systematic ID
SPBC685.09
Product
origin recognition complex subunit Orc2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
orp2
UniProt ID
Q09142
ORFeome ID
33/33C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2782036..2783676 forward strand

Annotation

GO biological process

GO:0006270 - DNA replication initiation

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GO cellular component

GO:0000785 - chromatin

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GO:0031261 - DNA replication preinitiation complex

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GO:0005664 - nuclear origin of replication recognition complex

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GO:0005656 - nuclear pre-replicative complex

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GO:0043596 - nuclear replication fork

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GO:0005634 - nucleus

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GO molecular function

GO:0003688 - DNA replication origin binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01455 - O-phosphorylated residue

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001425 - abnormal negative regulation of mitotic DNA replication initiation resulting in complete rereplication

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

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Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0001425 - abnormal negative regulation of mitotic DNA replication initiation resulting in complete rereplication

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Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000229 - cut

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Genotypes:

FYPO:0001311 - decreased plasmid loss

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Genotypes:

FYPO:0003950 - decreased protein localization to chromatin at replication origin

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Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

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Genotypes:

FYPO:0000377 - increased frequency of apoptosis

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

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Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

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Genotypes:

FYPO:0002409 - inviable after spore germination, single cell division, with elongated germ tube

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Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

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Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000012 - mitotic G2/M phase transition delay

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0000411 - normal mitotic cell cycle

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Genotypes:

FYPO:0001790 - normal plasmid loss

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Genotypes:

FYPO:0004962 - normal protein localization to chromatin at replication origin

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF04084RecA-like_ORC2RecA-like_ORC2PFAM
PF24882WHD_ORC2WHD_ORC2PFAM
PTHR14052ORIGIN RECOGNITION COMPLEX SUBUNIT 2ORC2PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.
Hart EA et al. Curr Genet 2002 Aug;41(5):342-8
PMID:12840006 - Xenopus origin recognition complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORC.
Kong D et al. EMBO J 2003 Jul 01;22(13):3441-50
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:9436991 - Negative regulation of Cdc18 DNA replication protein by Cdc2.
Lopez-Girona A et al. Mol Biol Cell 1998 Jan;9(1):63-73
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11689699 - Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit.
Kong D et al. Mol Cell Biol 2001 Dec;21(23):8095-103
PMID:10523506 - The fission yeast origin recognition complex is constitutively associated with chromatin and is differentially modified through the cell cycle.
Lygerou Z et al. J Cell Sci 1999 Nov;112 ( Pt 21):3703-12
PMID:11683912 - Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins.
Takahashi T et al. Genes Cells 2001 Oct;6(10):837-49
PMID:16371652 - Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.
Marchetti MA et al. J Cell Sci 2006 Jan 01;119(Pt 1):124-31
PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.
Locovei AM et al. Cell Div 2006 Nov 17;1:27
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11717425 - The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein.
Lee JK et al. Proc Natl Acad Sci U S A 2001 Nov 20;98(24):13589-94
PMID:18723846 - Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions.
Houchens CR et al. J Biol Chem 2008 Oct 31;283(44):30216-24
PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.
Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:11850415 - Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein.
Chuang RY et al. J Biol Chem 2002 May 10;277(19):16920-7
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:12419251 - Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication.
Wuarin J et al. Cell 2002 Nov 01;111(3):419-31
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:9177184 - Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast.
Brown GW et al. Proc Natl Acad Sci U S A 1997 Jun 10;94(12):6142-7
PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.
Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:10655214 - Functions of fission yeast orp2 in DNA replication and checkpoint control.
Kiely J et al. Genetics 2000 Feb;154(2):599-607
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:10535928 - Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe.
Moon KY et al. Proc Natl Acad Sci U S A 1999 Oct 26;96(22):12367-72
PMID:11486016 - Control of DNA rereplication via Cdc2 phosphorylation sites in the origin recognition complex.
Vas A et al. Mol Cell Biol 2001 Sep;21(17):5767-77
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:8552194 - Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein.
Leatherwood J et al. Nature 1996 Jan 25;379(6563):360-3