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protein coding gene - apl3 (SPBC691.03c) - AP-2 adaptor complex alpha subunit Apl3

Gene summary

Standard name
apl3
Systematic ID
SPBC691.03c
Product
AP-2 adaptor complex alpha subunit Apl3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pi033, SPACTOKYO_453.02c
UniProt ID
Q9C0W7
ORFeome ID
49/49E12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1424228..1427447 reverse strand

Annotation

GO biological process

GO:0072583 - clathrin-dependent endocytosis

References:

GO:0006886 - intracellular protein transport

References:

GO cellular component

GO:0030122 - AP-2 adaptor complex

References:

GO:0005938 - cell cortex

References:

GO:0051285 - cell cortex of cell tip

References:

GO:0032153 - cell division site

References:

GO:0005905 - clathrin-coated pit

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0035615 - clathrin-cargo adaptor activity

References:

Modification

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000423 - decreased rate of endocytosis during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0000079 - sensitive to caspofungin

References:

Genotypes:

FYPO:0003358 - sensitive to miconazole

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

Protein features

PBO:0111787 - adaptin family

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000743 - abnormal actin cortical patch internalization

References:

Genotypes:

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0000135 - abnormal plasma membrane sterol distribution

References:

Genotypes:

FYPO:0005508 - abnormal plasma membrane to vacuole transport

References:

Genotypes:

FYPO:0005503 - abnormally monopolar protein localization to cell tip

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Genotypes:

FYPO:0005515 - abolished protein localization to cell cortex, with protein mislocalized to vacuole, during vegetative growth

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

References:

Genotypes:

FYPO:0000745 - delayed onset of actin cortical patch internalization

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002318 - increased cellular ergosterol level

References:

Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0001945 - normal protein secretion

References:

Genotypes:

FYPO:0004247 - normal vacuolar morphology during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002766 - resistance to clotrimazole

References:

Genotypes:

FYPO:0002634 - resistance to cobalt

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000079 - sensitive to caspofungin

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003358 - sensitive to miconazole

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01602Adaptin_NClathrin/coatomer_adapt-like_NPfam
PF02296Alpha_adaptin_CClathrin_a-adaptin_app_sub_CPfam
PF02883Alpha_adaptinC2Clathrin_a/b/g-adaptin_app_IgPfam
SM00809Alpha_adaptinC2Clathrin_a/b/g-adaptin_app_IgSMART
G3DSA:1.25.10.10:FF:000020CATH-FunFam
G3DSA:1.25.10.10ARM-likeCATH-Gene3D
G3DSA:2.60.40.1230CATH-Gene3D
G3DSA:3.30.310.10TBP_dom_sfCATH-Gene3D
SSF48371ARM-type_foldSUPERFAMILY
SSF49348Clathrin_app_Ig-like_sfSUPERFAMILY
SSF55711Coatomer/calthrin_app_sub_CSUPERFAMILY
PTHR22780Adaptor_Complx_Large_SubunitPANTHER
PIRSF037091AP2_complex_alphaAP2_complex_asuPIRSF

Orthologs

References / Literature

PMID:26749213 - The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
de León N et al. Mol Microbiol 2016 May;100(3):409-24
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6