PomBase home

protein coding gene - prp1 (SPBC6B1.07) - U4/U6 x U5 tri-snRNP complex subunit Prp1

Gene summary

Standard name
prp1
Systematic ID
SPBC6B1.07
Product
U4/U6 x U5 tri-snRNP complex subunit Prp1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
zer1
UniProt ID
Q12381
ORFeome ID
47/47B06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2641921..2644897 forward strand

Annotation

Complementation

PBO:0004137 - does not functionally complement S. cerevisiae PRP6

References:

PBO:0123318 - is not functionally complemented by S. cerevisiae PRP6

References:

Disease association

MONDO:0013516 - retinitis pigmentosa 60

References:

GO biological process

GO:0045292 - mRNA cis splicing, via spliceosome

References:

GO:0000244 - spliceosomal tri-snRNP complex assembly

References:

GO cellular component

GO:0071013 - catalytic step 2 spliceosome

References:

GO:0005634 - nucleus

References:

GO:0046540 - U4/U6 x U5 tri-snRNP complex

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001908 - increased pre-mRNA level

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111755 - TPR repeat protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

References:

Genotypes:

FYPO:0000189 - abnormal mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0003602 - abolished mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003029 - decreased mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0002522 - decreased poly(A)+ mRNA export from nucleus

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0003041 - decreased snRNA splicing

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000911 - increased nuclear RNA level during vegetative growth

References:

Genotypes:

FYPO:0001908 - increased pre-mRNA level

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0002107 - inviable stubby vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF13181TPR_8TPR_rptPFAM
PF06424PRP1_NPRP1_NPFAM
SM00028tpr_5TPR_rptSMART
SM00386hat_new_1HATSMART
G3DSA:1.25.40.10:FF:000256FUNFAM
SSF48452TPR-likeTPR-like_helical_dom_sfSUPERFAMILY
G3DSA:1.25.40.10Tetratricopeptide repeat domainTPR-like_helical_dom_sfGENE3D
PTHR11246PRE-MRNA SPLICING FACTORSyf1-likePANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:9286671 - The prp1+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast.
Urushiyama S et al. Genetics 1997 Sep;147(1):101-15
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:9003295 - Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe.
Urushiyama S et al. Mol Gen Genet 1996 Nov 27;253(1-2):118-27
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:2034223 - prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.
Rosenberg GH et al. Mol Gen Genet 1991 Apr;226(1-2):305-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.
Bayne EH et al. Science 2008 Oct 24;322(5901):602-6
PMID:2721492 - Pre-mRNA splicing mutants of Schizosaccharomyces pombe.
Potashkin J et al. EMBO J 1989 Feb;8(2):551-9
PMID:11350031 - Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.
Habara Y et al. RNA 2001 May;7(5):671-81
PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.
Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:22240020 - Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast.
Watanabe N et al. Biochem Biophys Res Commun 2012 Feb 03;418(1):62-6
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20028739 - Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast.
Bernard P et al. Mol Cell Biol 2010 Mar;30(5):1145-57
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22990236 - Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin.
Shim YS et al. EMBO J 2012 Nov 28;31(23):4375-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27350684 - Mutant allele of rna14 in fission yeast affects pre-mRNA splicing.
Yadav S et al. J Genet 2016 Jun;95(2):389-97
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:11252721 - Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor.
Schwelnus W et al. EMBO Rep 2001 Jan;2(1):35-41
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20007600 - The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
Lützelberger M et al. Nucleic Acids Res 2010 Mar;38(5):1610-22
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.
Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.
Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:37637271 - Isolation of mutant alleles of the U6 snRNA m 6 A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe .
Willet AH et al. MicroPubl Biol 2023;2023
PMID:8862522 - A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.
Lundgren K et al. Mol Biol Cell 1996 Jul;7(7):1083-94
PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.
Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31
PMID:2798130 - Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants.
Potashkin J et al. Nucleic Acids Res 1989 Oct 11;17(19):7821-31
PMID:23754748 - Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.
Banerjee S et al. Mol Cell Biol 2013 Aug;33(16):3125-36
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26582768 - Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation.
Gal C et al. EMBO Rep 2016 Jan;17(1):79-93
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
PMID:17450151 - Distinct roles of HDAC complexes in promoter silencing, antisense suppression and DNA damage protection.
Nicolas E et al. Nat Struct Mol Biol 2007 May;14(5):372-80
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16133344 - Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe.
Bottner CA et al. Curr Genet 2005 Sep;48(3):151-61
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97