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protein coding gene - ofd1 (SPBC6B1.08c) - hypoxic oxygen sensor, prolyl-3,4-dihydroxylase Ofd1

Gene summary

Standard name
ofd1
Systematic ID
SPBC6B1.08c
Product
hypoxic oxygen sensor, prolyl-3,4-dihydroxylase Ofd1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
uS12
UniProt ID
Q11120
ORFeome ID
45/45A01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2644726..2646637 reverse strand

Annotation

GO biological process

GO:0071456 - cellular response to hypoxia

References:

GO:2000639 - negative regulation of SREBP signaling pathway

References:

GO:0032436 - positive regulation of proteasomal ubiquitin-dependent protein catabolic process

References:

GO:0006449 - regulation of translational termination

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0005506 - iron ion binding

References:

GO:0019826 - oxygen sensor activity

References:

GO:0031543 - peptidyl-proline dioxygenase activity

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GO:0005515 - protein binding

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GO:0140311 - protein sequestering activity

References:

Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001258 - normal protein level during cellular response to hypoxia

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006283 - abolished protein-proline dihydroxylation during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000658 - decreased DNA binding

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Genotypes:

FYPO:0001274 - decreased protein level during cellular response to hypoxia

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Genotypes:

FYPO:0003251 - decreased transcription from SRE promoter

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Genotypes:

FYPO:0000657 - increased DNA binding

References:

Genotypes:

FYPO:0001255 - increased protein level during cellular response to oxygen

References:

Genotypes:

FYPO:0008205 - increased protein localization to nucleus during hypoxia

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0001258 - normal protein level during cellular response to hypoxia

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000840 - normal RNA level

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0000022 - shmoo with long tip

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10637Ofd1_CTDDOxoglutarate/Fe-dep_Oase_CPFAM
PF136612OG-FeII_Oxy_4TPA1/OFD1_NPFAM
PS51471FE2OG_OXYOxoglu/Fe-dep_dioxygenase_domPROSITE_PROFILES
SM00702p4hcPro_4_hyd_alphSMART
G3DSA:2.60.120.620:FF:000014FUNFAM
G3DSA:2.60.120.620q2cbj1_9rhob like domainGENE3D
G3DSA:3.60.130.20TPA1/Ofd1_CGENE3D
PTHR12117HISTONE ACETYLTRANSFERASE COMPLEXuS12_prolyl_hydroxylasePANTHER

Orthologs

References / Literature

PMID:19139265 - The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.
Roberts-Galbraith RH et al. J Cell Biol 2009 Jan 12;184(1):113-27
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:19158663 - Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast.
Lee CY et al. EMBO J 2009 Jan 21;28(2):135-43
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18418381 - Oxygen-regulated degradation of fission yeast SREBP by Ofd1, a prolyl hydroxylase family member.
Hughes BT et al. EMBO J 2008 May 21;27(10):1491-501
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21610214 - Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O2-mediated gene control in S. pombe.
Rispal D et al. RNA 2011 Jul;17(7):1213-24
PMID:29083304 - Prolyl dihydroxylation of unassembled uS12/Rps23 regulates fungal hypoxic adaptation.
Clasen SJ et al. Elife 2017 Oct 30;6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:24327658 - Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis.
Brookheart RT et al. J Biol Chem 2014 Jan 31;289(5):2725-35
PMID:24550462 - Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy.
Loenarz C et al. Proc Natl Acad Sci U S A 2014 Mar 18;111(11):4019-24
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21481773 - The hypoxic regulator of sterol synthesis nro1 is a nuclear import adaptor.
Yeh TL et al. Structure 2011 Apr 13;19(4):503-14
PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22017871 - Regulation of the Sre1 hypoxic transcription factor by oxygen-dependent control of DNA binding.
Lee CY et al. Mol Cell 2011 Oct 21;44(2):225-34
GO_REF:0000002 - Comments
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6