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protein coding gene - nbs1 (SPBC6B1.09c) - Mre11 complex BRCT domain subunit Nbs1

Gene summary

Standard name
nbs1
Systematic ID
SPBC6B1.09c
Product
Mre11 complex BRCT domain subunit Nbs1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
slr10, slr8
UniProt ID
O43070
ORFeome ID
45/45A11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2648577..2650977 reverse strand

Annotation

Disease association

MONDO:0015909 - aplastic anemia

References:

MONDO:0013108 - leukemia, acute lymphocytic, susceptibility to, 1

References:

MONDO:0009623 - Nijmegen breakage syndrome

References:

GO biological process

GO:0006325 - chromatin organization

References:

GO:0000729 - DNA double-strand break processing

References:

GO:0006281 - DNA repair

References:

GO:0006302 - double-strand break repair

References:

GO:0000724 - double-strand break repair via homologous recombination

References:

GO:0006303 - double-strand break repair via nonhomologous end joining

References:

GO:0007095 - mitotic G2 DNA damage checkpoint signaling

References:

GO:0031573 - mitotic intra-S DNA damage checkpoint signaling

References:

GO:0000723 - telomere maintenance

References:

GO:0000722 - telomere maintenance via recombination

References:

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

GO:0030870 - Mre11 complex

References:

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

References:

GO molecular function

GO:0140463 - chromatin-protein adaptor activity

References:

GO:0003684 - damaged DNA binding

References:

GO:0140031 - phosphorylation-dependent protein binding

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0002472 - abolished histone H2A phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002471 - abolished protein phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0002907 - circularized chromosome during mitotic G1 phase

References:

Genotypes:

FYPO:0004867 - decreased histone H2A phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0004869 - decreased number of Crb2 foci during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006872 - increased histone H2A phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002596 - increased histone H2A phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0004656 - increased protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0006871 - increased protein phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0003906 - normal growth on bleomycin

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0003183 - normal growth on phleomycin

References:

Genotypes:

FYPO:0002601 - normal histone H2A phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0002905 - normal telomere maintenance

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0005549 - normal viability upon nitrogen starvation

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0002344 - sensitive to phleomycin

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0005404 - telomeric regions absent from linear chromosomes

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111779 - BRCT domain

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001352 - abnormal chromatin organization during vegetative growth

References:

Genotypes:

FYPO:0005268 - abnormal mitotic cell cycle regulation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0005453 - abolished break-induced loss of heterozygosity via chromosomal translocation

References:

Genotypes:

FYPO:0005057 - abolished meiotic DNA double-strand break clipping

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0004287 - decreased double-strand break repair via nonhomologous end joining

References:

Genotypes:

FYPO:0000185 - decreased gene conversion during vegetative growth

References:

Genotypes:

FYPO:0004867 - decreased histone H2A phosphorylation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0003179 - decreased intragenic meiotic recombination

References:

Genotypes:

FYPO:0005058 - decreased meiotic DNA double-strand break clipping

References:

Genotypes:

FYPO:0000218 - decreased Mre11 complex assembly

References:

Genotypes:

FYPO:0001382 - decreased protein kinase activity

References:

Genotypes:

FYPO:0002474 - decreased protein localization to double-strand break site

References:

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0005452 - increased break-induced loss of heterozygosity

References:

Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0004656 - increased protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001927 - normal cell cycle regulation during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0002169 - normal growth during cellular response to gamma radiation

References:

Genotypes:

FYPO:0004229 - normal growth during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0003183 - normal growth on phleomycin

References:

Genotypes:

FYPO:0005136 - normal meiotic DNA double-strand break formation

References:

Genotypes:

FYPO:0004606 - normal protein level during meiotic cell cycle

References:

Genotypes:

FYPO:0002554 - normal protein localization to double-strand break site

References:

Genotypes:

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0002344 - sensitive to phleomycin

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0005455 - telomere assembly at double-strand break site

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00498FHAFHA_domPfam
cd22667FHA_NBNCDD
PS50006FHA_DOMAINFHA_domPROSITE profiles
SM00240FHAFHA_domSMART
G3DSA:2.60.200.20:FF:000082CATH-FunFam
G3DSA:2.60.200.20CATH-Gene3D
G3DSA:3.40.50.10190BRCT_dom_sfCATH-Gene3D
G3DSA:3.40.50.11080CATH-Gene3D
SSF49879SMAD_FHA_dom_sfSUPERFAMILY
PTHR12162Nibrin-relPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:23080121 - Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair.
Limbo O et al. Nucleic Acids Res 2012 Dec;40(22):11435-49
PMID:18160711 - Recombination-based telomere maintenance is dependent on Tel1-MRN and Rap1 and inhibited by telomerase, Taz1, and Ku in fission yeast.
Subramanian L et al. Mol Cell Biol 2008 Mar;28(5):1443-55
PMID:23188080 - The fission yeast MRN complex tethers dysfunctional telomeres for NHEJ repair.
Reis CC et al. EMBO J 2012 Dec 12;31(24):4576-86
PMID:19752195 - Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
Milman N et al. Mol Cell Biol 2009 Nov;29(22):5998-6005
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:17936710 - Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.
Limbo O et al. Mol Cell 2007 Oct 12;28(1):134-46
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21441914 - ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
Williams GJ et al. Nat Struct Mol Biol 2011 Apr;18(4):423-31
PMID:19798055 - Failed gene conversion leads to extensive end processing and chromosomal rearrangements in fission yeast.
Tinline-Purvis H et al. EMBO J 2009 Nov 04;28(21):3400-12
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:17724078 - Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.
Cullen JK et al. Mol Cell Biol 2007 Nov;27(21):7745-57
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:21098122 - Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaks.
Limbo O et al. Mol Cell Biol 2011 Feb;31(3):573-83
PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.
Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:19804755 - Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.
Williams RS et al. Cell 2009 Oct 02;139(1):87-99
PMID:18854158 - Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.
Williams RS et al. Cell 2008 Oct 03;135(1):97-109
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:29851556 - Mre11-Rad50-dependent activity of ATM/Tel1 at DNA breaks and telomeres in the absence of Nbs1.
Limbo O et al. Mol Biol Cell 2018 Jun 01;29(11):1389-1399
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:20140190 - A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.
Subramanian L et al. PLoS Genet 2010 Feb 05;6(2):e1000839
PMID:23335786 - Spontaneous telomere to telomere fusions occur in unperturbed fission yeast cells.
Almeida H et al. Nucleic Acids Res 2013 Mar 01;41(5):3056-67
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30295604 - BRCT domains of the DNA damage checkpoint proteins TOPBP1/Rad4 display distinct specificities for phosphopeptide ligands.
Day M et al. Elife 2018 Oct 08;7
PMID:28292918 - Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe .
Li Y et al. Genetics 2017 May;206(1):481-496
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:19804756 - A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage.
Lloyd J et al. Cell 2009 Oct 02;139(1):100-11
PMID:22705791 - Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.
Schiller CB et al. Nat Struct Mol Biol 2012 Jun 17;19(7):693-700
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:18378696 - Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex.
Akamatsu Y et al. Mol Cell Biol 2008 Jun;28(11):3639-51
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12944481 - Molecular characterization of the Schizosaccharomyces pombe nbs1+ gene involved in DNA repair and telomere maintenance.
Ueno M et al. Mol Cell Biol 2003 Sep;23(18):6553-63
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:12944482 - The fission yeast Rad32 (Mre11)-Rad50-Nbs1 complex is required for the S-phase DNA damage checkpoint.
Chahwan C et al. Mol Cell Biol 2003 Sep;23(18):6564-73
PMID:20421724 - Phosphorylation-regulated binding of Ctp1 to Nbs1 is critical for repair of DNA double-strand breaks.
Dodson GE et al. Cell Cycle 2010 Apr 15;9(8):1516-22
PMID:33836577 - A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.
Zdravković A et al. Proc Natl Acad Sci U S A 2021 Mar 16;118(11)
PMID:19211838 - The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling.
Porter-Goff ME et al. Mol Biol Cell 2009 Apr;20(7):2096-107
PMID:20065069 - The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.
Willis N et al. Genetics 2010 Apr;184(4):887-97
PMID:15964794 - ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1.
You Z et al. Mol Cell Biol 2005 Jul;25(13):5363-79