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protein coding gene - psf2 (SPBC725.13c) - GINS complex subunit Psf2

Gene summary

Standard name
psf2
Systematic ID
SPBC725.13c
Product
GINS complex subunit Psf2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
dre13, bsh3
UniProt ID
O94329
ORFeome ID
05/05F08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1228148..1229187 reverse strand

Annotation

GO biological process

GO:0006270 - DNA replication initiation

References:

GO:0000727 - double-strand break repair via break-induced replication

References:

GO:0033260 - nuclear DNA replication

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0000811 - GINS complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002890 - abnormal horsetail nucleus morphology

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000625 - abnormal premeiotic DNA replication

References:

Genotypes:

FYPO:0008164 - abnormal protein localization to kinetochore during mitotic M phase

References:

Genotypes:

FYPO:0000913 - abnormal sporulation resulting in formation of ascus containing non-uniform spores

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0000316 - inviable after spore germination

References:

Genotypes:

FYPO:0001497 - inviable elongated cell with mitotic cell cycle arrest in interphase

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000012 - mitotic G2/M phase transition delay

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05916Sld5GINS_APFAM
PF25005PSF2_NPSF2_NPFAM
cd21694GINS_B_Psf2PSF2_NCDD
cd11712GINS_A_psf2CDD
G3DSA:3.40.5.50:FF:000001FUNFAM
G3DSA:1.20.58.1020:FF:000001FUNFAM
SSF158573GINS helical bundle-likeGINS_bundle-like_dom_sfSUPERFAMILY
SSF160059PriA/YqbF domainSUPERFAMILY
G3DSA:3.40.5.50GENE3D
G3DSA:1.20.58.1020GENE3D
PTHR12772DNA REPLICATION COMPLEX GINS PROTEIN PSF2GINS_Psf2PANTHER
PIRSF028998GINS_PSF2GINS_Psf2PIRSF

Orthologs

References / Literature

PMID:16199877 - Suppressors of Bir1p (Survivin) identify roles for the chromosomal passenger protein Pic1p (INCENP) and the replication initiation factor Psf2p in chromosome segregation.
Huang HK et al. Mol Cell Biol 2005 Oct;25(20):9000-15
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:21593208 - CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.
Fukuura M et al. Mol Biol Cell 2011 Jul 15;22(14):2620-33
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.
Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15941470 - Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.
Yang X et al. BMC Mol Biol 2005 Jun 07;6:13
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.
Dolan WP et al. Genetics 2010 May;185(1):39-53
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15466421 - A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.
Gómez EB et al. Genetics 2005 Jan;169(1):77-89
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:16990792 - Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.
Yabuuchi H et al. EMBO J 2006 Oct 04;25(19):4663-74
PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
Kanke M et al. EMBO J 2012 May 02;31(9):2182-94
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:18753627 - Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks.
Nitani N et al. Proc Natl Acad Sci U S A 2008 Sep 02;105(35):12973-8