protein coding gene - hsk1 (SPBC776.12c) - Dbf4(Dfp1)-dependent protein kinase Hsk1

Gene summary

Standard name

hsk1

Systematic ID

SPBC776.12c

Product

Dbf4(Dfp1)-dependent protein kinase Hsk1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

cdc7

UniProt ID

P50582

ORFeome ID

40/40F05

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3197060..3199201 reverse strand

Annotation

Protein features

Orthologs

• CDC7 (HGNC:1745) Homo sapiens
• hsk1 (SJAG_03160) Schizosaccharomyces japonicus
• CDC7 (YDL017W) Saccharomyces cerevisiae

References / Literature

PMID:21099360 - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.

Matsumoto S et al. Cell Cycle 2010 Dec 01;9(23):4627-37

PMID:10545452 - Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe.

Liu J et al. Genetics 1999 Nov;153(3):1193-203

PMID:22279046 - Rif1 is a global regulator of timing of replication origin firing in fission yeast.

Hayano M et al. Genes Dev 2012 Jan 15;26(2):137-50

PMID:23045396 - Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

Sabatinos SA et al. Mol Cell Biol 2012 Dec;32(24):4986-97

PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.

Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90

PMID:20230746 - Hsk1- and SCF(Pof3)-dependent proteolysis of S. pombe Ams2 ensures histone homeostasis and centromere function.

Takayama Y et al. Dev Cell 2010 Mar 16;18(3):385-96

PMID:11359920 - Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.

Takeda T et al. Mol Biol Cell 2001 May;12(5):1257-74

PMID:14508607 - Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 ( cdc7).

Snaith HA et al. Curr Genet 2003 Oct;44(1):38-48

PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.

Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87

PMID:11027263 - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.

Snaith HA et al. Mol Cell Biol 2000 Nov;20(21):7922-32

PMID:11402029 - Bipartite binding of a kinase activator activates Cdc7-related kinase essential for S phase.

Ogino K et al. J Biol Chem 2001 Aug 17;276(33):31376-87

PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.

Liang DT et al. Genetics 2001 Oct;159(2):471-86

PMID:10411894 - Cell cycle regulation of Dfp1, an activator of the Hsk1 protein kinase.

Brown GW et al. Proc Natl Acad Sci U S A 1999 Jul 20;96(15):8443-8

PMID:34608864 - DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.

Aricthota S et al. Elife 2021 Oct 05;10

PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.

Gómez EB et al. Genetics 2008 Jun;179(2):757-71

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:19422421 - Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast.

Shimmoto M et al. Genes Cells 2009 Jun;14(6):669-82

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:16263721 - Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex.

Matsumoto S et al. J Biol Chem 2005 Dec 30;280(52):42536-42

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:22024164 - Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.

Matsumoto S et al. J Cell Biol 2011 Oct 31;195(3):387-401

PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.

Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.

UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:27320927 - Rif1 Regulates the Fate of DNA Entanglements during Mitosis.

Zaaijer S et al. Cell Rep 2016 Jun 28;16(1):148-160

PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73

PMID:10886372 - The Schizosaccharomyces pombe spo6+ gene encoding a nuclear protein with sequence similarity to budding yeast Dbf4 is required for meiotic second division and sporulation.

Nakamura T et al. Genes Cells 2000 Jun;5(6):463-79

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:18799612 - The Hsk1(Cdc7) replication kinase regulates origin efficiency.

Patel PK et al. Mol Biol Cell 2008 Dec;19(12):5550-8

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:24656819 - Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity.

Davé A et al. Cell Rep 2014 Apr 10;7(1):53-61

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:18180284 - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.

Bailis JM et al. Mol Cell Biol 2008 Mar;28(5):1724-38

PMID:21095590 - DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin.

Furuya K et al. Mol Cell 2010 Nov 24;40(4):606-18

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:10409743 - A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage.

Takeda T et al. Mol Cell Biol 1999 Aug;19(8):5535-47

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:28069740 - Checkpoint-Independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 Kinase.

Matsumoto S et al. Mol Cell Biol 2017 Apr 01;37(7)

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:16199868 - Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.

Gómez EB et al. Mol Cell Biol 2005 Oct;25(20):8887-903

PMID:21518960 - Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.

Hayano M et al. Mol Cell Biol 2011 Jun;31(12):2380-91

PMID:15338237 - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.

Dolan WP et al. Chromosoma 2004 Sep;113(3):145-56

PMID:26201080 - The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.

Zech J et al. PLoS One 2015;10(7):e0132595

PMID:23862021 - The C-terminus of S. pombe DDK subunit Dfp1 is required for meiosis-specific transcription and cohesin cleavage.

Le AH et al. Biol Open 2013 Jul 15;2(7):728-38

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP et al. Genetics 2010 May;185(1):39-53

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.

Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:7621824 - hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication.

Masai H et al. EMBO J 1995 Jul 03;14(13):3094-104

PMID:16141239 - Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis.

Gachet Y et al. J Cell Sci 2005 Sep 15;118(Pt 18):4231-42

PMID:14625560 - Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres.

Bailis JM et al. Nat Cell Biol 2003 Dec;5(12):1111-6

PMID:12052858 - A conserved domain of Schizosaccharomyces pombe dfp1(+) is uniquely required for chromosome stability following alkylation damage during S phase.

Fung AD et al. Mol Cell Biol 2002 Jul;22(13):4477-90

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6