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protein coding gene - plh1 (SPBC776.14) - phospholipid-diacylglycerol acyltransferase Plh1/Lro1

Gene summary

Standard name
plh1
Systematic ID
SPBC776.14
Product
phospholipid-diacylglycerol acyltransferase Plh1/Lro1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
lro1
UniProt ID
O94680
ORFeome ID
49/49E01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3204400..3208235 forward strand

Annotation

PBO:0004404 - 2.3.1.158

Disease association

MONDO:0007620 - fish eye disease

References:

MONDO:0009515 - Norum disease

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GO biological process

GO:0046339 - diacylglycerol metabolic process

References:

GO:0140042 - lipid droplet formation

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GO:0019915 - lipid storage

References:

GO:0019432 - triglyceride biosynthetic process

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GO cellular component

GO:0032541 - cortical endoplasmic reticulum

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GO:0005783 - endoplasmic reticulum

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GO:0005789 - endoplasmic reticulum membrane

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GO:0097038 - perinuclear endoplasmic reticulum

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GO molecular function

GO:0046027 - phospholipid:diacylglycerol acyltransferase activity

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Modification

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0005663 - abnormal re-entry into mitotic cell cycle after arrest in response to heat shock

References:

Genotypes:

FYPO:0007343 - abolished lipid droplet formation

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0005585 - decreased cellular triglyceride level during vegetative growth

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Genotypes:

FYPO:0003959 - decreased diacylglycerol O-acyltransferase activity

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Genotypes:

FYPO:0006054 - decreased lipid droplet formation

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0003954 - decreased triglyceride biosynthetic process

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0007764 - enlarged lipid droplets surrounded by endoplasmic reticulum

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Genotypes:

FYPO:0003975 - fragmented nuclear envelope

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Genotypes:

FYPO:0006662 - fragmented nucleus during G0

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Genotypes:

FYPO:0008288 - increased cellular ceramide level

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Genotypes:

FYPO:0004695 - increased cellular diglyceride level

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Genotypes:

FYPO:0006289 - increased cellular phytoceramide level

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Genotypes:

FYPO:0004164 - increased cellular reactive oxygen species level in stationary phase

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Genotypes:

FYPO:0006276 - increased karyorrhexis during stationary phase

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Genotypes:

FYPO:0006275 - increased pyknosis during stationary phase

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0000944 - inviable spore with normal morphology

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0004925 - irregular ascospore wall

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0005231 - loss of viability in stationary phase upon glucose starvation

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0010042 - normal growth on pentadecanoic acid

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Genotypes:

FYPO:0001673 - normal nuclear morphology

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0004168 - normal viability in stationary phase during glucose starvation

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Genotypes:

FYPO:0004697 - sensitive to 1,2-dioctanoyl-sn-glycerol

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Genotypes:

FYPO:0004696 - sensitive to fatty acid

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0006974 - triglyceride absent from cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0005585 - decreased cellular triglyceride level during vegetative growth

References:

Genotypes:

FYPO:0003954 - decreased triglyceride biosynthetic process

References:

Genotypes:

FYPO:0007342 - decreased triglyceride level in lipid droplet

References:

Genotypes:

FYPO:0003960 - increased diacylglycerol O-acyltransferase activity

References:

Genotypes:

FYPO:0003955 - increased triglyceride biosynthetic process

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002236 - normal cellular sterol ester level

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Genotypes:

FYPO:0010042 - normal growth on pentadecanoic acid

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF02450LCATLACT/PDAT_acylTrfasePfam
G3DSA:3.40.50.1820:FF:000160CATH-FunFam
G3DSA:3.40.50.1820AB_hydrolase_foldCATH-Gene3D
SSF53474AB_hydrolase_foldSUPERFAMILY
PTHR11440PANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:26990381 - Lipid Droplets Form from Distinct Regions of the Cell in the Fission Yeast Schizosaccharomyces pombe.
Meyers A et al. Traffic 2016 Jun;17(6):657-69
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:30072439 - The putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast.
Nakazawa N et al. J Cell Sci 2018 Aug 21;131(16)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:40424131 - Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast.
Hoshikawa Y et al. Proc Natl Acad Sci U S A 2025 Jun 03;122(22):e2422126122
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25318672 - Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function.
He Y et al. Mol Biol Cell 2014 Dec 15;25(25):4083-95
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28282432 - Metabolic crosstalk between membrane and storage lipids facilitates heat stress management in Schizosaccharomyces pombe.
Péter M et al. PLoS One 2017;12(3):e0173739
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:28011631 - Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival.
Yang HJ et al. Biol Open 2017 Feb 15;6(2):217-222
PMID:12963726 - Schizosaccharomyces pombe cells deficient in triacylglycerols synthesis undergo apoptosis upon entry into the stationary phase.
Zhang Q et al. J Biol Chem 2003 Nov 21;278(47):47145-55
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18653539 - Caspase-dependent and -independent lipotoxic cell-death pathways in fission yeast.
Low CP et al. J Cell Sci 2008 Aug 15;121(Pt 16):2671-84
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704