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protein coding gene - cnp20 (SPBC800.13) - CENP-T histone fold, kinetochore scaffold protein

Gene summary

Standard name
cnp20
Systematic ID
SPBC800.13
Product
CENP-T histone fold, kinetochore scaffold protein
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9HGK9
ORFeome ID
23/23B12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 279483..281247 forward strand

Annotation

Disease association

MONDO:0032875 - short stature and microcephaly with genital anomalies

References:

GO biological process

GO:0006325 - chromatin organization

References:

GO:0051382 - kinetochore assembly

References:

GO:0000070 - mitotic sister chromatid segregation

References:

GO cellular component

GO:0061838 - CENP-T-W-S-X complex

References:

GO:0000775 - chromosome, centromeric region

References:

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0000939 - inner kinetochore

References:

GO:0000776 - kinetochore

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0140463 - chromatin-protein adaptor activity

References:

GO:0003677 - DNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

Protein features

PBO:0111763 - histone fold

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004213 - abnormal attachment of mitotic spindle microtubules to kinetochore

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0001946 - abolished mitotic sister chromatid separation

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Genotypes:

FYPO:0000634 - abolished protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000450 - decreased protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001571 - increased protein-protein interaction

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Genotypes:

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0001796 - protein mislocalized to centromere

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF15511CENP-T_CCENP-T/H4_CPfam
cd22920HFD_CENP-TCDD
G3DSA:1.10.20.10:FF:000105CATH-FunFam
G3DSA:1.10.20.10Histone-foldCATH-Gene3D
SSF47113Histone-foldSUPERFAMILY
PTHR46904CENP-TPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22561346 - CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Schleiffer A et al. Nat Cell Biol 2012 May 06;14(6):604-13
PMID:10759889 - Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library.
Ding DQ et al. Genes Cells 2000 Mar;5(3):169-90
PMID:25619765 - The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast.
Folco HD et al. Curr Biol 2015 Feb 02;25(3):348-356
PMID:27666591 - Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading.
Dong Q et al. Mol Cell 2016 Oct 06;64(1):79-91
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26275423 - Inner Kinetochore Protein Interactions with Regional Centromeres of Fission Yeast.
Thakur J et al. Genetics 2015 Oct;201(2):543-61
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.
Jian Y et al. J Cell Sci 2023 Jan 15;136(2)
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34810257 - Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.
Dong Q et al. Proc Natl Acad Sci U S A 2021 Nov 30;118(48)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
GO_REF:0000051 - S. pombe keyword mapping
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.
Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235
PMID:36705602 - Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.
Virant D et al. J Cell Biol 2023 Apr 03;222(4)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.
Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44
PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.
Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43