protein coding gene - tas3 (SPBC83.03c) - RITS complex linker subunit tas3

Gene summary

Standard name

tas3

Systematic ID

SPBC83.03c

Product

RITS complex linker subunit tas3

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O94687

ORFeome ID

34/34F08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1514006..1516179 reverse strand

Annotation

Protein features

Orthologs

• TNRC6A (HGNC:11969) Homo sapiens
• TNRC6B (HGNC:29190) Homo sapiens
• TNRC6C (HGNC:29318) Homo sapiens
• tas3 (SJAG_03042) Schizosaccharomyces japonicus

References / Literature

PMID:20211136 - Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity.

Bayne EH et al. Cell 2010 Mar 05;140(5):666-77

PMID:18345014 - Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.

Zhang K et al. Nat Struct Mol Biol 2008 Apr;15(4):381-8

PMID:29866182 - RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.

Okazaki K et al. Epigenetics Chromatin 2018 Jun 04;11(1):26

PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.

Okita AK et al. Commun Biol 2019;2:17

PMID:17531816 - Functional separation of the requirements for establishment and maintenance of centromeric heterochromatin.

Partridge JF et al. Mol Cell 2007 May 25;26(4):593-602

PMID:19394293 - An alpha motif at Tas3 C terminus mediates RITS cis spreading and promotes heterochromatic gene silencing.

Li H et al. Mol Cell 2009 Apr 24;34(2):155-67

PMID:21211723 - Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci.

Yamane K et al. Mol Cell 2011 Jan 07;41(1):56-66

PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.

Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55

PMID:32499408 - The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast.

Sun L et al. J Cell Sci 2020 Jul 07;133(13)

PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.

Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)

PMID:26438724 - H3K9 methylation extends across natural boundaries of heterochromatin in the absence of an HP1 protein.

Stunnenberg R et al. EMBO J 2015 Nov 12;34(22):2789-803

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

InterPro:IPR019486 - Unknown title

PMID:21060862 - Continuous requirement for the Clr4 complex but not RNAi for centromeric heterochromatin assembly in fission yeast harboring a disrupted RITS complex.

Shanker S et al. PLoS Genet 2010 Oct 28;6(10):e1001174

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:20705239 - The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.

Gerace EL et al. Mol Cell 2010 Aug 13;39(3):360-72

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:25730778 - Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex.

Holoch D et al. Nat Struct Mol Biol 2015 Apr;22(4):328-35

PMID:20178743 - Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Halic M et al. Cell 2010 Feb 19;140(4):504-16

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:19362535 - High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin.

Schalch T et al. Mol Cell 2009 Apr 10;34(1):36-46

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:19164572 - Msc1 links dynamic Swi6/HP1 binding to cell fate determination.

Lawrence RJ et al. Proc Natl Acad Sci U S A 2009 Jan 27;106(4):1163-8

PMID:24095277 - Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.

Marasovic M et al. Mol Cell 2013 Oct 24;52(2):173-83

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:15475954 - RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing.

Noma K et al. Nat Genet 2004 Nov;36(11):1174-80

PMID:15607976 - Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

Motamedi MR et al. Cell 2004 Dec 17;119(6):789-802

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.

Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9

Pfam:PF10427 - Unknown title

PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.

Yu Y et al. Sci Rep 2016 Aug 19;6:31752

PMID:15743828 - RNA interference (RNAi)-dependent and RNAi-independent association of the Chp1 chromodomain protein with distinct heterochromatic loci in fission yeast.

Petrie VJ et al. Mol Cell Biol 2005 Mar;25(6):2331-46

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:22081013 - The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.

Schalch T et al. Nat Struct Mol Biol 2011 Nov 13;18(12):1351-7

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:14704433 - RNAi-mediated targeting of heterochromatin by the RITS complex.

Verdel A et al. Science 2004 Jan 30;303(5658):672-6

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:22727667 - Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.

Ishida M et al. Mol Cell 2012 Jul 27;47(2):228-41

PMID:22733737 - Ers1 links HP1 to RNAi.

Rougemaille M et al. Proc Natl Acad Sci U S A 2012 Jul 10;109(28):11258-63

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:25972440 - Global regulation of heterochromatin spreading by Leo1.

Verrier L et al. Open Biol 2015 May;5(5)

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:17891150 - A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain.

Till S et al. Nat Struct Mol Biol 2007 Oct;14(10):897-903

PMID:22895252 - A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.

Kawakami K et al. Genes Dev 2012 Aug 15;26(16):1811-24

PMID:26443059 - Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast.

Deng X et al. Sci Rep 2015 Oct 07;5:14707

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:15615848 - RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production.

Sugiyama T et al. Proc Natl Acad Sci U S A 2005 Jan 04;102(1):152-7