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protein coding gene - apc15 (SPBC83.04) - anaphase-promoting complex platform subcomplex scaffold subunit Apc15

Gene summary

Standard name
apc15
Systematic ID
SPBC83.04
Product
anaphase-promoting complex platform subcomplex scaffold subunit Apc15
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94688
ORFeome ID
04/04D07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1516696..1517574 forward strand

Annotation

GO biological process

GO:0031145 - anaphase-promoting complex-dependent catabolic process

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GO cellular component

GO:0005680 - anaphase-promoting complex

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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Multi-locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0004318 - abolished mitotic spindle assembly checkpoint

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Genotypes:

FYPO:0005928 - abolished protein polyubiquitination during vegetative growth

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0003585 - decreased anaphase-promoting complex-dependent protein catabolic process

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0004367 - normal mitotic spindle assembly

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005781 - decreased duration of mitotic spindle assembly checkpoint

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Genotypes:

FYPO:0004102 - decreased protein polyubiquitination during vegetative growth

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Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0007385 - increased duration of protein-protein interaction

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0005783 - normal mitotic checkpoint complex assembly

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05841Apc15pAPC_su15/mnd2_NPFAM
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15060174 - Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Schwickart M et al. Mol Cell Biol 2004 Apr;24(8):3562-76
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.
Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:12477395 - Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
Yoon HJ et al. Curr Biol 2002 Dec 10;12(23):2048-54
PMID:16950791 - Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.
Yoon HJ et al. J Biol Chem 2006 Oct 27;281(43):32284-93
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.
Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39
PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.
May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.
Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220