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protein coding gene - its8 (SPBC839.08c) - pig-N

Gene summary

Standard name
its8
Systematic ID
SPBC839.08c
Product
pig-N
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q8WZK2
ORFeome ID
47/47F02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 613331..616604 reverse strand

Annotation

Disease association

MONDO:0013563 - multiple congenital anomalies-hypotonia-seizures syndrome 1

References:

GO biological process

GO:0006506 - GPI anchor biosynthetic process

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GO cellular component

GO:0005789 - endoplasmic reticulum membrane

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GO molecular function

GO:0051377 - mannose-ethanolamine phosphotransferase activity

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0008260 - decreased protein localization to cell surface, protein mislocalized to endoplasmic reticulum

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0000951 - inviable small vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001470 - normal growth on tacrolimus

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Genotypes:

FYPO:0004561 - resistance to BE49385A

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Genotypes:

FYPO:0001966 - sensitive to aculeacin A

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Genotypes:

FYPO:0004560 - sensitive to BE49385A

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Genotypes:

FYPO:0002720 - sensitive to beta-glucanase

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Genotypes:

FYPO:0000105 - sensitive to cyclosporin A

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Genotypes:

FYPO:0002641 - sensitive to micafungin

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Genotypes:

FYPO:0000086 - sensitive to tacrolimus

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Genotypes:

FYPO:0000647 - vegetative cell lysis

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Genotypes:

FYPO:0001496 - viable elongated multiseptate vegetative cell

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Genotypes:

FYPO:0004559 - viable swollen spheroid vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01663PhosphodiestPhosphodiest/P_Trfase_domPfam
PF04987PigNGPI_EtnP_transferase_1_CPfam
cd16020GPI_EPT_1PIGN_NCDD
G3DSA:3.40.720.10:FF:000015CATH-FunFam
G3DSA:3.40.720.10Alkaline_phosphatase_core_sfCATH-Gene3D
SSF53649Alkaline_phosphatase_core_sfSUPERFAMILY
PTHR12250GPI_EtnP_transferase_1PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11297516 - Its8, a fission yeast homolog of Mcd4 and Pig-n, is involved in GPI anchor synthesis and shares an essential function with calcineurin in cytokinesis.
Yada T et al. J Biol Chem 2001 Apr 27;276(17):13579-86
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:12637524 - Zinc finger protein Prz1 regulates Ca2+ but not Cl- homeostasis in fission yeast. Identification of distinct branches of calcineurin signaling pathway in fission yeast.
Hirayama S et al. J Biol Chem 2003 May 16;278(20):18078-84
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:22848669 - Studies on the roles of clathrin-mediated membrane trafficking and zinc transporter Cis4 in the transport of GPI-anchored proteins in fission yeast.
Jaiseng W et al. PLoS One 2012;7(7):e41946
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:17881729 - Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast.
Takada H et al. Mol Biol Cell 2007 Dec;18(12):4794-802
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:20531409 - Global coordination of transcriptional control and mRNA decay during cellular differentiation.
Amorim MJ et al. Mol Syst Biol 2010 Jun 08;6:380