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protein coding gene - thf1 (SPBC839.16) - C1-5,6,7,8-tetrahydrofolate (THF) synthase, trifunctional enzyme Thf1

Gene summary

Standard name
thf1
Systematic ID
SPBC839.16
Product
C1-5,6,7,8-tetrahydrofolate (THF) synthase, trifunctional enzyme Thf1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q8WZJ7
ORFeome ID
30/30B02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 628002..631710 forward strand

Annotation

PBO:0003277 - 1.5.1.5

PBO:0003276 - 3.5.4.9

PBO:0003275 - 6.3.4.3

Comment

PBO:0003274 - ssDNA binding within synthetase domain

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Disease association

MONDO:0060611 - combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia

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MONDO:0001860 - folic acid deficiency anemia

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MONDO:0011120 - neural tube defects, folate-sensitive

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GO biological process

GO:0009257 - 10-formyltetrahydrofolate biosynthetic process

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GO:0046656 - folic acid biosynthetic process

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GO:0006730 - one-carbon metabolic process

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GO:0035999 - tetrahydrofolate interconversion

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GO cellular component

GO:0005829 - cytosol

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GO molecular function

GO:0005524 - ATP binding

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GO:0004329 - formate-tetrahydrofolate ligase activity

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GO:0004477 - methenyltetrahydrofolate cyclohydrolase activity

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GO:0004488 - methylenetetrahydrofolate dehydrogenase (NADP+) activity

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GO:0003697 - single-stranded DNA binding

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Modification

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02882THF_DHG_CYH_CTHF_DH/CycHdrlase_NAD-bd_domPFAM
PF01268FTHFSFormate_THF_ligasePFAM
PF00763THF_DHG_CYHTHF_DH/CycHdrlase_cat_domPFAM
cd00477FTHFSFormate_THF_ligaseCDD
cd01080NAD_bind_m-THF_DH_CyclohydTHF_DH/CycHdrlase_NAD-bd_domCDD
PS00721FTHFS_1Formate_THF_ligase_CSPROSITE_PATTERNS
PS00722FTHFS_2Formate_THF_ligase_CSPROSITE_PATTERNS
PR00085THFDHDRGNASETHF_DH/CycHdrlasePRINTS
G3DSA:3.10.410.10:FF:000001FUNFAM
G3DSA:3.40.50.10860:FF:000005FUNFAM
G3DSA:3.40.50.720:FF:000006FUNFAM
G3DSA:3.40.50.300:FF:001123FUNFAM
G3DSA:3.40.50.300:FF:000245FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF53223Aminoacid dehydrogenase-like, N-terminal domainAminoacid_DH-like_N_sfSUPERFAMILY
SSF51735NAD(P)-binding Rossmann-fold domainsNAD(P)-bd_dom_sfSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:3.40.50.720GENE3D
G3DSA:3.10.410.10Formyltetrahydrofolate synthetase, domain 3GENE3D
G3DSA:3.40.50.10860Leucine Dehydrogenase, chain A, domain 1GENE3D
PTHR48099C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATEDPANTHER
MF_01543FTHFSFormate_THF_ligaseHAMAP
MF_01576THF_DHG_CYHTHF_DH/CycHdrlaseHAMAP

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:25633902 - Characterization and review of MTHFD1 deficiency: four new patients, cellular delineation and response to folic and folinic acid treatment.
Burda P et al. J Inherit Metab Dis 2015 Sep;38(5):863-72
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:8226914 - Single-stranded DNA binding activity of C1-tetrahydrofolate synthase enzymes.
Wahls WP et al. J Biol Chem 1993 Nov 15;268(32):23792-8
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684