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protein coding gene - pus2 (SPBC887.11) - cytosolic tRNA pseudouridine(1), mitochondrial tRNA pseudouridine(27/28) synthase Pus2

Gene summary

Standard name
pus2
Systematic ID
SPBC887.11
Product
cytosolic tRNA pseudouridine(1), mitochondrial tRNA pseudouridine(27/28) synthase Pus2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94295
ORFeome ID
44/44E01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3559275..3561112 forward strand

Annotation

PBO:0004474 - 4.2.1.70

Disease association

MONDO:0024553 - myopathy, lactic acidosis, and sideroblastic anemia 1

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GO biological process

GO:1990481 - mRNA pseudouridine synthesis

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GO:0031119 - tRNA pseudouridine synthesis

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GO cellular component

GO:0005739 - mitochondrion

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GO:0005634 - nucleus

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GO molecular function

GO:0160153 - mitochondrial tRNA pseudouridine(27/28) synthase activity

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GO:0003723 - RNA binding

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GO:0106029 - tRNA pseudouridine synthase activity

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01416PseudoU_synth_1PsdUridine_synth_TruA_a/b_domPFAM
cd02568PseudoU_synth_PUS1_PUS2PUS1/PUS2-likeCDD
G3DSA:3.30.70.580:FF:000002FUNFAM
G3DSA:3.30.70.660:FF:000015FUNFAM
SSF55120Pseudouridine synthasePsdUridine_synth_cat_dom_sfSUPERFAMILY
G3DSA:3.30.70.660PsdUridine_synth_TruA_CGENE3D
G3DSA:3.30.70.580TruA/RsuA/RluB/E/F_NGENE3D
PTHR11142PSEUDOURIDYLATE SYNTHASEPsdUridine_synth_TruAPANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
TIGR00071hisT_truAPsdUridine_synth_TruANCBIFAM

Orthologs

References / Literature

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
GO_REF:0000002 - Comments
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40829803 - MoDorado: enhanced detection of tRNA modifications in nanopore sequencing by off-label use of modification callers.
Rübsam FNM et al. Nucleic Acids Res 2025 Aug 11;53(15)
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12