protein coding gene - pfh1 (SPBC887.14c) - mitochondrial 5' to 3' DNA helicase Pif1/Pfh1 OR nuclear 5' to 3' DNA helicase Pif1/Pfh1

Gene summary

Standard name

pfh1

Systematic ID

SPBC887.14c

Product

mitochondrial 5' to 3' DNA helicase Pif1/Pfh1 OR nuclear 5' to 3' DNA helicase Pif1/Pfh1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

pif1

UniProt ID

Q9UUA2

ORFeome ID

33/33H02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3567593..3570476 reverse strand

Annotation

Protein features

Orthologs

• PIF1 (HGNC:26220) Homo sapiens
• pfh1 (SJAG_03780) Schizosaccharomyces japonicus
• RRM3 (YHR031C) Saccharomyces cerevisiae
• PIF1 (YML061C) Saccharomyces cerevisiae

References / Literature

GO_REF:0000104 - Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features.

PMID:15576681 - Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.

Tanaka H et al. Nucleic Acids Res 2004;32(21):6367-77

PMID:12058079 - Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases.

Zhou JQ et al. Mol Biol Cell 2002 Jun;13(6):2180-91

PMID:22426535 - The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.

Steinacher R et al. Genes Dev 2012 Mar 15;26(6):594-602

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.

Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.

Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626

PMID:34228709 - Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.

Soriano I et al. PLoS Genet 2021 Jul;17(7):e1009526

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:32692737 - CRL4Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation.

Zhang JM et al. PLoS Genet 2020 Jul;16(7):e1008933

PMID:25303777 - The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening.

McDonald KR et al. DNA Repair (Amst) 2014 Dec;24:80-86

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:22308326 - Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility.

Zamir L et al. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):E406-14

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:22426534 - DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.

Sabouri N et al. Genes Dev 2012 Mar 15;26(6):581-93

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:30053106 - The Pif1 signature motif of Pfh1 is necessary for both protein displacement and helicase unwinding activities, but is dispensable for strand-annealing activity.

Mohammad JB et al. Nucleic Acids Res 2018 Sep 19;46(16):8516-8531

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:15302919 - Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast.

Ryu GH et al. Nucleic Acids Res 2004;32(14):4205-16

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.

UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531

PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.

Jalan M et al. Elife 2019 Jan 22;8

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:25203555 - Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.

Zhang JM et al. PLoS Biol 2014 Sep;12(9):e1001946

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:12409464 - The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase.

Tanaka H et al. Nucleic Acids Res 2002 Nov 01;30(21):4728-39

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.

Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58

PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.

Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515

PMID:27185885 - G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.

Wallgren M et al. Nucleic Acids Res 2016 Jul 27;44(13):6213-31

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:28054200 - The functions of the multi-tasking Pfh1 Pif1 helicase.

Sabouri N Curr Genet 2017 Aug;63(4):621-626

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:22347400 - A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1.

Chisholm KM et al. PLoS One 2012;7(2):e30748

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:32093266 - Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family.

Muellner J et al. Genes (Basel) 2020 Feb 20;11(2)

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:18725402 - The Schizosaccharomyces pombe Pfh1p DNA helicase is essential for the maintenance of nuclear and mitochondrial DNA.

Pinter SF et al. Mol Cell Biol 2008 Nov;28(21):6594-608

PMID:25471935 - The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage.

Sabouri N et al. BMC Biol 2014 Dec 04;12:101

PMID:25417108 - Dicer promotes transcription termination at sites of replication stress to maintain genome stability.

Castel SE et al. Cell 2014 Oct 23;159(3):572-83

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97