PomBase home

protein coding gene - rft1 (SPBC887.19) - ER membrane protein required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER Rft1

Gene summary

Standard name
rft1
Systematic ID
SPBC887.19
Product
ER membrane protein required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER Rft1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94302
ORFeome ID
45/45C04
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome II: 3580173..3583740 forward strand

Annotation

Disease association

MONDO:0015286 - congenital disorder of glycosylation

References:

MONDO:0005066 - metabolic disease

References:

MONDO:0012783 - RFT1-congenital disorder of glycosylation

References:

GO biological process

GO:0006488 - dolichol-linked oligosaccharide biosynthetic process

References:

GO:0034203 - glycolipid translocation

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

References:

GO molecular function

GO:0034202 - glycolipid floppase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001991 - inviable after spore germination, without cell division

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04506Rft-1RFT1Pfam
cd13130MATE_rft1CDD
PTHR13117RFT1PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PB_REF:0000003 - Disease Association Curation
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87