PomBase home

protein coding gene - ecl3 (SPBC8E4.12c) - TORC1 inhibitor, Ecl3

Gene summary

Standard name
ecl3
Systematic ID
SPBC8E4.12c
Product
TORC1 inhibitor, Ecl3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPNG1995-96
UniProt ID
C6Y4C3
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4441340..4441827 reverse strand

Annotation

GO biological process

GO:1904262 - negative regulation of TORC1 signaling

References:

GO:0055062 - phosphate ion homeostasis

References:

GO molecular function

GO:0004860 - protein kinase inhibitor activity

References:

GO:0030291 - protein serine/threonine kinase inhibitor activity

References:

Multi-locus phenotype

FYPO:0008320 - abnormal cell morphology during sulphur deprivation

References:

Genotypes:

FYPO:0007120 - abolished cell cycle arrest in mitotic G1 phase in response to leucine starvation

References:

Genotypes:

FYPO:0008066 - abolished cell cycle arrest in mitotic G1 phase in response to phosphate starvation

References:

Genotypes:

FYPO:0007500 - abolished macroautophagy during sulfur starvation

References:

Genotypes:

FYPO:0007087 - abolished sporulation during sulfur starvation

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0008064 - decreased mating efficiency during phosphate starvation

References:

Genotypes:

FYPO:0006331 - decreased mating efficiency during stationary phase

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0007084 - decreased RNA level during cellular response to sulfur starvation

References:

Genotypes:

FYPO:0006334 - decreased RNA level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0002944 - decreased RNA level during G0

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0008065 - decreased sporulation frequency during phosphate starvation

References:

Genotypes:

FYPO:0000712 - delayed onset of cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0007086 - elongated vegetative cell during sulfur starvation

References:

Genotypes:

FYPO:0007093 - increased level of ribosomal protein gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0008427 - increased protein level during cellular response to sulfur starvation

References:

Genotypes:

FYPO:0008428 - increased protein phosphorylation during sulfur starvation

References:

Genotypes:

FYPO:0007085 - increased RNA level during cellular response to sulfur starvation

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007119 - loss of viability upon amino acid starvation

References:

Genotypes:

FYPO:0007792 - loss of viability upon magnesium starvation

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0007088 - loss of viability upon sulfur starvation

References:

Genotypes:

FYPO:0007089 - normal cell cycle arrest at mitotic G2/M phase transition during sulfur starvation

References:

Genotypes:

FYPO:0002048 - normal cell morphology during nitrogen starvation

References:

Genotypes:

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

FYPO:0004993 - normal spore germination frequency

References:

Genotypes:

FYPO:0002052 - normal sporulation frequency

References:

Genotypes:

FYPO:0005549 - normal viability upon nitrogen starvation

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0002780 - decreased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0002143 - decreased cellular reactive oxygen species level in stationary phase

References:

Genotypes:

FYPO:0002173 - increased level of meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0002172 - increased level of nitrogen starvation gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0002664 - increased level of stress responsive gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0001043 - increased mating efficiency

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000588 - increased sporulation frequency

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

FYPO:0004168 - normal viability in stationary phase during glucose starvation

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Warnings

PBO:0000082 - new gene

References:

Protein features

IDNameInterPro nameDB name
PF12855Ecl1Ecl1/2/3PFAM

Orthologs

References / Literature

PMID:39105351 - A novel transcription factor Sdr1 involving sulfur depletion response in fission yeast.
Ohtsuka H et al. Genes Cells 2024 Aug;29(8):667-680
PMID:40395999 - Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast.
Ohtsuka H et al. Autophagy Rep 2022;1(1):51-64
PMID:24696293 - Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe.
Shimasaki T et al. Mol Genet Genomics 2014 Aug;289(4):685-93
PMID:28160081 - Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast.
Shimasaki T et al. Mol Genet Genomics 2017 Apr;292(2):475-481
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:36779416 - The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast.
Ohtsuka H et al. J Cell Sci 2023 Mar 15;136(6)
PMID:39910760 - Novel TORC1 inhibitor Ecl1 is regulated by phosphorylation in fission yeast.
Ohtsuka H et al. Aging Cell 2025 Feb 05;:e14450
PMID:33711009 - Structure-function analysis of fission yeast cleavage and polyadenylation factor (CPF) subunit Ppn1 and its interactions with Dis2 and Swd22.
Benjamin B et al. PLoS Genet 2021 Mar;17(3):e1009452
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:32282918 - Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3' processing and transcription termination that functions via its effects on CTD phosphatase Ssu72.
Sanchez AM et al. Nucleic Acids Res 2020 May 21;48(9):4811-4826
PMID:28388826 - Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome.
Ohtsuka H et al. Mol Microbiol 2017 Jul;105(1):84-97
PMID:36882296 - Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.
Sanchez AM et al. RNA 2023 Jun;29(6):808-825
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33064910 - Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast.
Shimasaki T et al. Genes Cells 2020 Dec;25(12):825-830
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33970532 - Magnesium depletion extends fission yeast lifespan via general amino acid control activation.
Ohtsuka H et al. Microbiologyopen 2021 Mar;10(2):e1176
PMID:31456006 - Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1.
Ohtsuka H et al. Mol Genet Genomics 2019 Dec;294(6):1499-1509
PMID:22212525 - Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast.
Ohtsuka H et al. Genes Cells 2012 Jan;17(1):39-52
PMID:38899862 - Suppression of inositol pyrophosphate toxicosis and hyper-repression of the fission yeast PHO regulon by loss-of-function mutations in chromatin remodelers Snf22 and Sol1.
Schwer B et al. mBio 2024 Jun 20;:e0125224
PMID:19352039 - Identification of Ecl family genes that extend chronological lifespan in fission yeast.
Ohtsuka H et al. Biosci Biotechnol Biochem 2009 Apr 23;73(4):885-9
PMID:21072667 - hsf1 (+) extends chronological lifespan through Ecl1 family genes in fission yeast.
Ohtsuka H et al. Mol Genet Genomics 2011 Jan;285(1):67-77
PMID:25204792 - Sexual development of Schizosaccharomyces pombe is induced by zinc or iron limitation through Ecl1 family genes.
Ohtsuka H et al. Mol Genet Genomics 2015 Feb;290(1):173-85
PMID:40063661 - Fission yeast Caprin protein is required for efficient heterochromatin establishment.
Zhang H et al. PLoS Genet 2025 Mar 10;21(3):e1011620
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:31276588 - Inositol pyrophosphates impact phosphate homeostasis via modulation of RNA 3' processing and transcription termination.
Sanchez AM et al. Nucleic Acids Res 2019 Sep 19;47(16):8452-8469
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:38133430 - Genetic suppressor screen identifies Tgp1 (glycerophosphocholine transporter), Kcs1 (IP 6 kinase), and Plc1 (phospholipase C) as determinants of inositol pyrophosphate toxicosis in fission yeast.
Bednor L et al. mBio 2023 Dec 22;:e0306223