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protein coding gene - dsc1 (SPBC947.10) - Golgi Dsc E3 ligase complex subunit Dsc1

Gene summary

Standard name
dsc1
Systematic ID
SPBC947.10
Product
Golgi Dsc E3 ligase complex subunit Dsc1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43085
ORFeome ID
27/27H06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 652950..655246 reverse strand

Annotation

GO biological process

GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

References:

GO:0032933 - SREBP signaling pathway

References:

GO:0043162 - ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

References:

GO cellular component

GO:0044695 - Dsc E3 ubiquitin ligase complex

References:

GO:0005783 - endoplasmic reticulum

References:

GO:0005794 - Golgi apparatus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0061630 - ubiquitin protein ligase activity

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00506 - N-linked glycan core N4-glycosylated asparagine

References:

Multi-locus phenotype

FYPO:0001669 - abolished protein processing during vegetative growth

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

Protein features

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111743 - zinc finger protein

Protein sequence feature

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001910 - abnormal protein glycosylation during vegetative growth

References:

Genotypes:

FYPO:0001424 - abolished protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001669 - abolished protein processing during vegetative growth

References:

Genotypes:

FYPO:0001913 - abolished ubiquitin ligase activity

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0003935 - decreased protein localization to Golgi apparatus, with protein mislocalized to endoplasmic reticulum

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0009095 - increased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0005262 - increased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0009076 - increased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000836 - increased protein level

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002448 - normal Dsc complex assembly

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0002332 - normal protein localization to Golgi apparatus

References:

Genotypes:

FYPO:0001423 - normal protein targeting to vacuole

References:

Genotypes:

FYPO:0000601 - normal vacuolar transport

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002634 - resistance to cobalt

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF11145DUF2921DUF2921Pfam
PF12678zf-rbx1Znf_RING_H2Pfam
cd23117RING-H2_TUL1-likeCDD
PS50089ZF_RING_2Znf_RINGPROSITE profiles
SM00184RINGZnf_RINGSMART
G3DSA:3.30.40.10:FF:001167CATH-FunFam
G3DSA:3.30.40.10Znf_RING/FYVE/PHDCATH-Gene3D
SSF57850SUPERFAMILY
PTHR22763HRD1_E3_ubiq-ligasesPANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:28202541 - Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.
Burr R et al. J Biol Chem 2017 Mar 31;292(13):5311-5324
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:26545776 - Identification of Rbd2 as a candidate protease for sterol regulatory element binding protein (SREBP) cleavage in fission yeast.
Kim J et al. Biochem Biophys Res Commun 2015 Dec 25;468(4):606-10
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:23760507 - Subunit architecture of the Golgi Dsc E3 ligase required for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast.
Lloyd SJ et al. J Biol Chem 2013 Jul 19;288(29):21043-21054
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:23729666 - Structural requirements for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast.
Chong R et al. J Biol Chem 2013 Jul 12;288(28):20351-60
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.
Le TK et al. J Cell Sci 2023 Oct 01;136(19)
PMID:25918164 - Endoplasmic Reticulum Exit of Golgi-resident Defective for SREBP Cleavage (Dsc) E3 Ligase Complex Requires Its Activity.
Raychaudhuri S et al. J Biol Chem 2015 Jun 05;290(23):14430-40
PMID:22086920 - Yeast sterol regulatory element-binding protein (SREBP) cleavage requires Cdc48 and Dsc5, a ubiquitin regulatory X domain-containing subunit of the Golgi Dsc E3 ligase.
Stewart EV et al. J Biol Chem 2012 Jan 02;287(1):672-681
PMID:28821619 - Dsc E3 ligase localization to the Golgi requires the ATPase Cdc48 and cofactor Ufd1 for activation of sterol regulatory element-binding protein in fission yeast.
Burr R et al. J Biol Chem 2017 Sep 29;292(39):16333-16350
GO_REF:0000002 - Comments
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:27655872 - A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP.
Hwang J et al. EMBO J 2016 Nov 02;35(21):2332-2349