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protein coding gene - clc1 (SPBC9B6.08) - clathrin light chain Clc1

Gene summary

Standard name
clc1
Systematic ID
SPBC9B6.08
Product
clathrin light chain Clc1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
git9
UniProt ID
Q9USP6
ORFeome ID
40/40A04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1827153..1828198 forward strand

Annotation

GO biological process

GO:0072583 - clathrin-dependent endocytosis

References:

GO:0006886 - intracellular protein transport

References:

GO cellular component

GO:0030479 - actin cortical patch

References:

GO:0030130 - clathrin coat of trans-Golgi network vesicle

References:

GO:0071439 - clathrin complex

References:

GO:0005737 - cytoplasm

References:

GO:0031410 - cytoplasmic vesicle

References:

GO:0030139 - endocytic vesicle

References:

GO:0005794 - Golgi apparatus

References:

GO:0005798 - Golgi-associated vesicle

References:

GO:0005886 - plasma membrane

References:

GO molecular function

GO:0032050 - clathrin heavy chain binding

References:

GO:0005198 - structural molecule activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000423 - decreased rate of endocytosis during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000174 - abnormal cell wall biogenesis

References:

Genotypes:

FYPO:0002023 - abnormal septum morphology during vegetative growth

References:

Genotypes:

FYPO:0002815 - abolished protein localization to cell surface

References:

Genotypes:

FYPO:0002159 - decreased 1,3-beta-D-glucan synthase activity

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001082 - decreased cell wall beta-glucan level

References:

Genotypes:

FYPO:0001235 - decreased extent of cell population growth

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002630 - irregular cell wall during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002258 - small vacuoles present in increased numbers

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01086Clathrin_lg_chClathrin_L-chainPfam
PS00581CLATHRIN_LIGHT_CHN_2Clathrin_L-chainPROSITE patterns
PTHR10639Clathrin_L-chainPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:25963819 - Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.
Lucena R et al. J Cell Biol 2015 May 11;209(3):387-402
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26749213 - The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
de León N et al. Mol Microbiol 2016 May;100(3):409-24
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15546162 - pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast.
Matsuyama A et al. Yeast 2004 Nov;21(15):1289-305
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:23977061 - Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
de León N et al. PLoS One 2013;8(8):e71510
PMID:31341193 - Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome.
Yanguas F et al. Sci Rep 2019 Jul 24;9(1):10747
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:2157626 - Isolation and characterization of mutants constitutive for expression of the fbp1 gene of Schizosaccharomyces pombe.
Hoffman CS et al. Genetics 1990 Apr;124(4):807-16
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:42691030 - The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe.
Karika LO et al. PLoS One 2026;21(9):e0357276
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:25977474 - A role for F-BAR protein Rga7p during cytokinesis in S. pombe.
Arasada R et al. J Cell Sci 2015 Jul 01;128(13):2259-68
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20587778 - Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast.
Sirotkin V et al. Mol Biol Cell 2010 Aug 15;21(16):2894-904
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
GO_REF:0000002 - Comments
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87