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protein coding gene - mcs2 (SPBP16F5.02) - transcription factor TFIIH complex cyclin Mcs2

Gene summary

Standard name
mcs2
Systematic ID
SPBP16F5.02
Product
transcription factor TFIIH complex cyclin Mcs2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P36613
ORFeome ID
32/32E06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1892980..1894408 forward strand

Annotation

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:0006367 - transcription initiation at RNA polymerase II promoter

References:

GO cellular component

GO:0005634 - nucleus

References:

GO:0070985 - transcription factor TFIIK complex

References:

GO molecular function

GO:0061575 - cyclin-dependent protein serine/threonine kinase activator activity

References:

GO:0005515 - protein binding

References:

GO:0016251 - RNA polymerase II general transcription initiation factor activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0002066 - inviable elongated aseptate cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001124 - normal vegetative cell size

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111794 - cyclin

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003171 - binucleate monoseptate cell with mitotic cell cycle arrest before cell separation

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0002463 - inviable branched, curved, elongated vegetative cell

References:

Genotypes:

FYPO:0002411 - inviable curved elongated vegetative cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0003848 - sensitive to hydrazinocurcumin

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00134Cyclin_NCyclin_NPfam
PF16899Cyclin_C_2Cyclin_C_2Pfam
cd20524CYCLIN_CCNH_rpt1CDD
cd20525CYCLIN_CCNH_rpt2CDD
SM00385CYCLINCyclin-like_domSMART
G3DSA:1.10.472.10:FF:000095CATH-FunFam
G3DSA:1.10.472.10CATH-Gene3D
SSF47954Cyclin-like_sfSUPERFAMILY
PTHR10026Cyclin/Ssn8PANTHER
TIGR00569ccl1CyclinH/Ccl1NCBIFAM

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15555586 - Mcs2 and a novel CAK subunit Pmh1 associate with Skp1 in fission yeast.
Bamps S et al. Biochem Biophys Res Commun 2004 Dec 24;325(4):1424-32
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.
Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15829570 - Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.
Lee KM et al. Mol Biol Cell 2005 Jun;16(6):2734-45
PMID:9857180 - Fission yeast Csk1 is a CAK-activating kinase (CAKAK).
Hermand D et al. EMBO J 1998 Dec 15;17(24):7230-8
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:8467814 - Characterization of the fission yeast mcs2 cyclin and its associated protein kinase activity.
Molz L et al. EMBO J 1993 Apr;12(4):1723-32
PMID:18309270 - Genome-wide drug-induced haploinsufficient screening of fission yeast for identification of hydrazinocurcumin targets.
Baek ST et al. J Microbiol Biotechnol 2008 Feb;18(2):263-9
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:8557036 - Schizosaccharomyces pombe Mop1-Mcs2 is related to mammalian CAK.
Damagnez V et al. EMBO J 1995 Dec 15;14(24):6164-72
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:8557037 - Identification of a cdk-activating kinase in fission yeast.
Buck V et al. EMBO J 1995 Dec 15;14(24):6173-83
GO_REF:0000002 - Comments
PMID:10226032 - Cdc2 activation in fission yeast depends on Mcs6 and Csk1, two partially redundant Cdk-activating kinases (CAKs).
Lee KM et al. Curr Biol 1999 Apr 22;9(8):441-4
PMID:14534314 - Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.
Spahr H et al. J Biol Chem 2003 Dec 19;278(51):51301-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:2474475 - cdc2 and the regulation of mitosis: six interacting mcs genes.
Molz L et al. Genetics 1989 Aug;122(4):773-82