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protein coding gene - ubc7 (SPBP16F5.04) - Hrd1 ubiquitin ligase complex ubiquitin conjugating enzyme E2 Ubc7

Gene summary

Standard name
ubc7
Systematic ID
SPBP16F5.04
Product
Hrd1 ubiquitin ligase complex ubiquitin conjugating enzyme E2 Ubc7
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ubcP3, ubc3
UniProt ID
O00102
ORFeome ID
32/32B06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1905688..1907025 forward strand

Annotation

PBO:0000272 - 6.3.2.19

GO biological process

GO:0036503 - ERAD quality control pathway

References:

GO:0180027 - inner nuclear membrane-associated protein degradation pathway

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005783 - endoplasmic reticulum

References:

GO:0005634 - nucleus

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GO:0000151 - ubiquitin ligase complex

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GO molecular function

GO:0005515 - protein binding

References:

GO:0061631 - ubiquitin conjugating enzyme activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001146 - decreased misfolded glycoprotein degradation during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00179UQ_conUBCPfam
cd23796UBCc_UBE2G2CDD
PS00183UBC_1UBQ-conjugating_ASPROSITE patterns
PS50127UBC_2UBCPROSITE profiles
SM00212UBCcSMART
G3DSA:3.10.110.10:FF:000008CATH-FunFam
G3DSA:3.10.110.10UBQ-conjugating_enzyme/RWDCATH-Gene3D
SSF54495UBQ-conjugating_enzyme/RWDSUPERFAMILY
PTHR24067Ub_conjugating_enzyme-E2-likePANTHER

Orthologs

References / Literature

PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.
Le TK et al. J Cell Sci 2023 Oct 01;136(19)
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:9154838 - A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.
Osaka F et al. Mol Cell Biol 1997 Jun;17(6):3388-97
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:28162934 - S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.
Lv Z et al. Mol Cell 2017 Feb 16;65(4):699-714.e6
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19520858 - Degradation of sterol regulatory element-binding protein precursor requires the endoplasmic reticulum-associated degradation components Ubc7 and Hrd1 in fission yeast.
Hughes BT et al. J Biol Chem 2009 Jul 31;284(31):20512-21
PMID:22083275 - N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe.
Mukaiyama H et al. Appl Microbiol Biotechnol 2012 Feb;93(4):1609-18
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:35075549 - Schizosaccharomyces pombe Fzo1 is subjected to the ubiquitin-proteasome-mediated degradation during the stationary phase.
Ahmad F et al. Int Microbiol 2022 May;25(2):397-404
PMID:31397327 - Crystal structure of the Schizosaccharomyces pombe U7BR E2-binding region in complex with Ubc7.
Hann ZS et al. Acta Crystallogr F Struct Biol Commun 2019 Aug 01;75(Pt 8):552-560
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7