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protein coding gene - mts4 (SPBP19A11.03c) - 19S proteasome regulatory subunit Rpn1/Mts4

Gene summary

Standard name
mts4
Systematic ID
SPBP19A11.03c
Product
19S proteasome regulatory subunit Rpn1/Mts4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
rpn1, ptp1
UniProt ID
P87048
ORFeome ID
49/49F03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2851121..2854102 reverse strand

Annotation

Complementation

PBO:0091806 - functionally complemented by human PSMD2

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GO biological process

GO:0010498 - proteasomal protein catabolic process

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GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

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GO cellular component

GO:1905754 - ascospore-type prospore nucleus

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GO:0005829 - cytosol

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GO:0034399 - nuclear periphery

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GO:0005634 - nucleus

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GO:0000502 - proteasome complex

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GO:0005838 - proteasome regulatory particle

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GO:0008540 - proteasome regulatory particle, base subcomplex

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GO:0034515 - proteasome storage granule

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GO molecular function

GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000359 - abnormal mitochondrial morphology

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Genotypes:

FYPO:0004944 - decreased mitochondrial membrane potential

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Genotypes:

FYPO:0004085 - decreased vegetative cell growth

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Genotypes:

FYPO:0000637 - increased cell population growth rate on glucose carbon source

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Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

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Genotypes:

FYPO:0006477 - abnormal mitotic metaphase/anaphase transition

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Genotypes:

FYPO:0002568 - abolished protein localization to nuclear periphery

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0004944 - decreased mitochondrial membrane potential

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0005142 - decreased proteasome core complex binding

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

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Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0004603 - inviable after spore germination, without cell division, elongated cell

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0003881 - mtDNA absent from cell

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001409 - normal growth on glycerol carbon source

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Genotypes:

FYPO:0002081 - normal growth on TPCK

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Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0007629 - normal viability during G0

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Genotypes:

FYPO:0000784 - protein mislocalized to nucleus during vegetative growth

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0004025 - resistance to carbendazim

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Genotypes:

FYPO:0000767 - resistance to staurosporine

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01851PC_repProteasome/cyclosome_rptPfam
PF17781RPN1_RPN2_NRPN1_NPfam
PF18051RPN1_CRPN1_CPfam
G3DSA:1.25.10.10:FF:000026CATH-FunFam
G3DSA:1.25.10.10ARM-likeCATH-Gene3D
SSF48371ARM-type_foldSUPERFAMILY
PTHR10943PANTHER
PIRSF01596526S_Psome_Rpn126S_Psome_Rpn1PIRSF
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.
Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36
PMID:9325304 - Mts4, a non-ATPase subunit of the 26 S protease in fission yeast is essential for mitosis and interacts directly with the ATPase subunit Mts2.
Wilkinson CR et al. J Biol Chem 1997 Oct 10;272(41):25768-77
PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.
Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38
PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.
Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)
PMID:11584278 - Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.
Wilkinson CR et al. Nat Cell Biol 2001 Oct;3(10):939-43
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:20231270 - Schizosaccharomyces pombe Dss1p is a DNA damage checkpoint protein that recruits Rad24p, Cdc25p, and Rae1p to DNA double-strand breaks.
Selvanathan SP et al. J Biol Chem 2010 May 07;285(19):14122-33
PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.
Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7
PMID:24938783 - Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.
Leitner A et al. Proc Natl Acad Sci U S A 2014 Jul 01;111(26):9455-60
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.
Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121
PMID:9822592 - Localization of the 26S proteasome during mitosis and meiosis in fission yeast.
Wilkinson CR et al. EMBO J 1998 Nov 16;17(22):6465-76
PMID:16149916 - Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis.
Jossé L et al. Biochem J 2006 Jan 01;393(Pt 1):303-9
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:19818717 - The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.
Sha Z et al. Mol Cell 2009 Oct 09;36(1):141-52
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:12783882 - Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly.
Yen HC et al. J Biol Chem 2003 Aug 15;278(33):30669-76
PMID:9727008 - The Pad1+ gene encodes a subunit of the 26 S proteasome in fission yeast.
Penney M et al. J Biol Chem 1998 Sep 11;273(37):23938-45
PMID:21182284 - Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) family.
McLean JR et al. J Proteome Res 2011 Mar 04;10(3):1208-15
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:27966061 - UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1.
Poulsen EG et al. Cell Stress Chaperones 2017 Jan;22(1):143-154
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:39473973 - Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity.
Ruiz-Romero G et al. iScience 2024 Nov 15;27(11):111095
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:10809753 - Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised.
Wilkinson CR et al. J Biol Chem 2000 May 19;275(20):15182-92
PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.
Penney M et al. PLoS One 2012;7(11):e50796
PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.
Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:21091378 - Txl1 and Txc1 are co-factors of the 26S proteasome in fission yeast.
Andersen KM et al. Antioxid Redox Signal 2011 May 01;14(9):1601-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22173095 - Reduced protein stability of human DJ-1/PARK7 L166P, linked to autosomal recessive Parkinson disease, is due to direct endoproteolytic cleavage by the proteasome.
Alvarez-Castelao B et al. Biochim Biophys Acta 2012 Feb;1823(2):524-33
PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.
Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898
PMID:12615927 - Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.
Seeger M et al. J Biol Chem 2003 May 09;278(19):16791-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12553909 - Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome.
Yen HC et al. Cell 2003 Jan 24;112(2):207-17
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:10872838 - Identification of a 26S proteasome-associated UCH in fission yeast.
Li T et al. Biochem Biophys Res Commun 2000 May 27;272(1):270-5
PMID:12006658 - Sum1, a component of the fission yeast eIF3 translation initiation complex, is rapidly relocalized during environmental stress and interacts with components of the 26S proteasome.
Dunand-Sauthier I et al. Mol Biol Cell 2002 May;13(5):1626-40
PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.
Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:35974206 - A context-dependent and disordered ubiquitin-binding motif.
Dreier JE et al. Cell Mol Life Sci 2022 Aug 16;79(9):484
PMID:15533439 - Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast.
Stone M et al. J Mol Biol 2004 Nov 26;344(3):697-706
PMID:16096059 - Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8.
Takeda K et al. Cell 2005 Aug 12;122(3):393-405
PMID:22906049 - Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation.
Boehringer J et al. Biochem J 2012 Nov 15;448(1):55-65
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:41068765 - Proteasome regulation of petite-negativity in fission yeast.
Amberg KL et al. BMC Biol 2025 Oct 09;23(1):302
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:25306921 - Dss1 is a 26S proteasome ubiquitin receptor.
Paraskevopoulos K et al. Mol Cell 2014 Nov 06;56(3):453-461
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:20614012 - HIV-1 replication through hHR23A-mediated interaction of Vpr with 26S proteasome.
Li G et al. PLoS One 2010 Jun 29;5(6):e11371
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:34634819 - Translational activators and mitoribosomal isoforms cooperate to mediate mRNA-specific translation in Schizosaccharomyces pombe mitochondria.
Herbert CJ et al. Nucleic Acids Res 2021 Nov 08;49(19):11145-11166
PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.
Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:20976105 - Silencing mediated by the Schizosaccharomyces pombe HIRA complex is dependent upon the Hpc2-like protein, Hip4.
Anderson HE et al. PLoS One 2010 Oct 18;5(10):e13488
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053