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protein coding gene - mis15 (SPBP22H7.09c) - CENP-N ortholog Mis15

Gene summary

Standard name
mis15
Systematic ID
SPBP22H7.09c
Product
CENP-N ortholog Mis15
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pi022, SPACTOKYO_453.12
UniProt ID
Q9C0W0
ORFeome ID
50/50F02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1448815..1450395 reverse strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0000776 - kinetochore

References:

GO:0031511 - Mis6-Sim4 complex

References:

GO:0005634 - nucleus

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0008014 - decreased protein localization to CENP-A containing chromatin during mitosis

References:

Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0007295 - increased protein localization to CENP-A containing chromatin

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0007234 - normal protein localization to centromere central core

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF05238CENP-NChl4/mis15/CENP-NPfam
G3DSA:3.10.20.720CATH-Gene3D

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.
Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928
PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.
Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.
Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98
PMID:34460892 - Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.
Tarhan Ç et al. Genet Mol Biol 2021;44(3):e20200245
PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.
Jian Y et al. J Cell Sci 2023 Jan 15;136(2)
PMID:30967422 - Negative Regulation of the Mis17-Mis6 Centromere Complex by mRNA Decay Pathway and EKC/KEOPS Complex in Schizosaccharomyces pombe .
Xu X et al. G3 (Bethesda) 2019 Jun 05;9(6):1815-1823
PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
Hayashi T et al. Cell 2004 Sep 17;118(6):715-29
PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .
Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21445296 - Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.
Shiroiwa Y et al. PLoS One 2011 Mar 21;6(3):e17761
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .
Gao C et al. Genetics 2017 Oct;207(2):559-569
PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.
Hirai H et al. Commun Biol 2022 Aug 15;5(1):818