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protein coding gene - rpa2 (SPBP23A10.07) - DNA-directed RNA polymerase I complex subunit Rpa2

Gene summary

Standard name
rpa2
Systematic ID
SPBP23A10.07
Product
DNA-directed RNA polymerase I complex subunit Rpa2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P7X8
ORFeome ID
48/48E06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2008086..2011955 forward strand

Annotation

PBO:0000445 - 2.7.7.6

Disease association

MONDO:0030067 - Treacher Collins syndrome 4

References:

GO biological process

GO:0042790 - nucleolar large rRNA transcription by RNA polymerase I

References:

GO:0006363 - termination of RNA polymerase I transcription

References:

GO:0006362 - transcription elongation by RNA polymerase I

References:

GO:0006361 - transcription initiation at RNA polymerase I promoter

References:

GO cellular component

GO:0005736 - RNA polymerase I complex

References:

GO molecular function

GO:0003677 - DNA binding

References:

GO:0003899 - DNA-directed RNA polymerase activity

References:

GO:0032549 - ribonucleoside binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00562RNA_pol_Rpb2_6DNA-dir_RNAP_su2_domPfam
PF04560RNA_pol_Rpb2_7RNA_pol_Rpb2_7Pfam
PF04561RNA_pol_Rpb2_2RNA_pol_Rpb2_2Pfam
PF04563RNA_pol_Rpb2_1RNA_pol_bsu_protrusionPfam
PF04565RNA_pol_Rpb2_3RNA_pol_Rpb2_3Pfam
PF06883RNA_pol_Rpa2_4Rpa2_dom_4Pfam
cd00653RNA_pol_B_RPB2DNA-dir_RNA_pol_su2CDD
PS01166RNA_POL_BETARNA_pol_bsu_CSPROSITE patterns
G3DSA:2.40.270.10:FF:000006CATH-FunFam
G3DSA:2.40.270.10:FF:000011CATH-FunFam
G3DSA:2.40.50.150:FF:000004CATH-FunFam
G3DSA:3.90.1100.10:FF:000008CATH-FunFam
G3DSA:3.90.1100.10:FF:000016CATH-FunFam
G3DSA:3.90.1110.10:FF:000007CATH-FunFam
G3DSA:2.40.270.10DNA-dir_RNAP_su2_hyb_sfCATH-Gene3D
G3DSA:2.40.50.150RNA_pol_RPB2_OB-foldCATH-Gene3D
G3DSA:3.90.1100.10CATH-Gene3D
G3DSA:3.90.1110.10RNA_pol_Rpb2_2_sfCATH-Gene3D
G3DSA:3.90.1800.10CATH-Gene3D
SSF64484SUPERFAMILY
PTHR20856DNA-dir_RNA_pol_su2PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27401558 - The Nrd1-like protein Seb1 coordinates cotranscriptional 3' end processing and polyadenylation site selection.
Lemay JF et al. Genes Dev 2016 Jul 01;30(13):1558-72
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25009287 - Megadalton-node assembly by binding of Skb1 to the membrane anchor Slf1.
Deng L et al. Mol Biol Cell 2014 Sep 01;25(17):2660-8
PMID:21095590 - DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin.
Furuya K et al. Mol Cell 2010 Nov 24;40(4):606-18
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:24818994 - The S. pombe translation initiation factor eIF4G is Sumoylated and associates with the SUMO protease Ulp2.
Jongjitwimol J et al. PLoS One 2014;9(5):e94182
PMID:35783577 - Generation and characterization of a temperature-sensitive mutant allele of the second largest subunit of RNA polymerase I in Schizosaccharomyces pombe .
Ishida K et al. MicroPubl Biol 2022;2022
PMID:41542823 - Analysis of Treacher Collins syndrome 4-associated mutations in Schizosaccharomyces pombe.
Kawakami K et al. FEBS Open Bio 2026 Jan 16;
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:33536435 - Conserved strategies of RNA polymerase I hibernation and activation.
Heiss FB et al. Nat Commun 2021 Feb 03;12(1):758
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000002 - Comments
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:37851576 - The LARP1 homolog Slr1p controls the stability and expression of proto-5'TOP mRNAs in fission yeast.
Mansouri-Noori F et al. Cell Rep 2023 Oct 31;42(10):113226
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74