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protein coding gene - orc4 (SPBP23A10.13) - origin recognition complex subunit Orc4

Gene summary

Standard name
orc4
Systematic ID
SPBP23A10.13
Product
origin recognition complex subunit Orc4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
orp4
UniProt ID
Q9Y794
ORFeome ID
34/34A02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2025388..2028676 forward strand

Annotation

Comment

PBO:0017015 - Orc4 binding footprint at replication origins is extended in G1 in the presence of Cdc1 and the MCM complex

References:

PBO:0017014 - Orc4 binds to the T-rich strand of replication origins throughout the cell cycle

References:

PBO:0014599 - Orc4 determines sequence specificity of ORC binding to DNA replication origins

References:

Disease association

MONDO:0016817 - Meier-Gorlin syndrome

References:

MONDO:0013428 - Meier-Gorlin syndrome 2

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GO biological process

GO:0006270 - DNA replication initiation

References:

GO:0006265 - DNA topological change

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0005664 - nuclear origin of replication recognition complex

References:

GO:0005656 - nuclear pre-replicative complex

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GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

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GO molecular function

GO:0016887 - ATP hydrolysis activity

References:

GO:0003688 - DNA replication origin binding

References:

GO:0030674 - protein-macromolecule adaptor activity

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Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01149 - sumoylated lysine

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Protein features

PBO:0111792 - AAA family ATPase

PBO:0111888 - AT hook protein

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Qualitative gene expression

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

References:

Genotypes:

FYPO:0001095 - abolished DNA replication origin binding

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000158 - DNA content increased during vegetative growth

References:

Genotypes:

FYPO:0002281 - inviable after spore germination with abnormal germ tube morphology

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Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002273 - inviable vegetative cell with abnormal cell morphology

References:

Genotypes:

FYPO:0002974 - normal origin recognition complex assembly

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02178AT_hookAT_hook_DNA-bd_motifPfam
PF13191AAA_16AAA_16Pfam
PF14629ORC4_CORC4_CPfam
cd00009AAACDD
SM00382AAAAAA+_ATPaseSMART
SM00384AT_hookAT_hook_DNA-bd_motifSMART
PR00929ATHOOKAT_hook_DNA-bd_motifPRINTS
G3DSA:3.40.50.300:FF:001597CATH-FunFam
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR12087ORC4PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:18723846 - Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions.
Houchens CR et al. J Biol Chem 2008 Oct 31;283(44):30216-24
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:11683912 - Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins.
Takahashi T et al. Genes Cells 2001 Oct;6(10):837-49
PMID:11717425 - The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein.
Lee JK et al. Proc Natl Acad Sci U S A 2001 Nov 20;98(24):13589-94
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:10077566 - The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks.
Chuang RY et al. Proc Natl Acad Sci U S A 1999 Mar 16;96(6):2656-61
PMID:15598736 - Atomic force microscopic analysis of the binding of the Schizosaccharomyces pombe origin recognition complex and the spOrc4 protein with origin DNA.
Gaczynska M et al. Proc Natl Acad Sci U S A 2004 Dec 28;101(52):17952-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.
Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11850415 - Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein.
Chuang RY et al. J Biol Chem 2002 May 10;277(19):16920-7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.
Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:11689699 - Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit.
Kong D et al. Mol Cell Biol 2001 Dec;21(23):8095-103
PMID:10535928 - Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe.
Moon KY et al. Proc Natl Acad Sci U S A 1999 Oct 26;96(22):12367-72
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:12840006 - Xenopus origin recognition complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORC.
Kong D et al. EMBO J 2003 Jul 01;22(13):3441-50
PMID:12374757 - Site-specific ORC binding, pre-replication complex assembly and DNA synthesis at Schizosaccharomyces pombe replication origins.
Kong D et al. EMBO J 2002 Oct 15;21(20):5567-76
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.
Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:29036220 - Defective replication initiation results in locus specific chromosome breakage and a ribosomal RNA deficiency in yeast.
Sanchez JC et al. PLoS Genet 2017 Oct;13(10):e1007041
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees