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protein coding gene - dpb2 (SPBP8B7.14c) - DNA polymerase epsilon catalytic subunit B, Dpb2

Gene summary

Standard name
dpb2
Systematic ID
SPBP8B7.14c
Product
DNA polymerase epsilon catalytic subunit B, Dpb2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94263
ORFeome ID
36/36H09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3656748..3658930 reverse strand

Annotation

GO biological process

GO:0006270 - DNA replication initiation

References:

GO:1902983 - DNA strand elongation involved in mitotic DNA replication

References:

GO:0042276 - error-prone translesion synthesis

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0008622 - epsilon DNA polymerase complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003690 - double-stranded DNA binding

References:

GO:0003697 - single-stranded DNA binding

References:

Modification

MOD:00696 - phosphorylated residue

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

References:

Genotypes:

FYPO:0005095 - abolished protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0006734 - delayed onset of protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0006661 - fragmented nucleus

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0002724 - inviable after spore germination, single or double cell division, elongated cell

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0004962 - normal protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04042DNA_pol_E_BDNA_pol_a/d/e_bsuPfam
PF29084OB_POLE2OB_POLE2Pfam
PTHR12708POLE2PANTHER

Orthologs

References / Literature

PMID:15388803 - Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.
Spiga MG et al. Nucleic Acids Res 2004;32(16):4945-53
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
Kanke M et al. EMBO J 2012 May 02;31(9):2182-94
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16723501 - Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication.
Taricani L et al. Mol Biol Cell 2006 Aug;17(8):3456-68
PMID:12925774 - Schizosacchromyces pombe Dpb2 binds to origin DNA early in S phase and is required for chromosomal DNA replication.
Feng W et al. Mol Biol Cell 2003 Aug;14(8):3427-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.