protein coding gene - thi20 (SPBP8B7.17c) - phosphomethylpyrimidine kinase Thi20

Gene summary

Standard name: thi20
Systematic ID: SPBP8B7.17c
Product: phosphomethylpyrimidine kinase Thi20
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O94265
ORFeome ID: 24/24F08
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome II: 3665670..3667739 reverse strand

Annotation

GO biological process

GO:0009228 - thiamine biosynthetic process

References:

GO_REF:0000033

GO:0009229 - thiamine diphosphate biosynthetic process

References:

GO_REF:0000041

GO cellular component

GO:0005737 - cytoplasm

References:

PMID:16823372

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO molecular function

GO:0008902 - hydroxymethylpyrimidine kinase activity

References:

GO_REF:0000033

GO:0008972 - phosphomethylpyrimidine kinase activity

References:

GO_REF:0000033

Genome organisation

PBO:0000758 - tandem duplication

Multi-locus phenotype

FYPO:0002243 - increased acid phosphatase activity

References:

PMID:34967420

Genotypes:

FYPO:0003267 - normal acid phosphatase activity

References:

PMID:34967420

Genotypes:

pho4delta thi20-G271D(aa)

Protein features

PBO:0111917 - TENA/THI domain

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

PMID:23697806

Genotypes:

thi20delta

FYPO:0002243 - increased acid phosphatase activity

References:

PMID:34967420

Genotypes:

thi20-G271D(aa)

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

PMID:23697806

Genotypes:

thi20delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

thi20delta

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

ID	Name	InterPro name	DB name
PF08543	Phos_pyr_kin	PM/HMP-P_kinase-1	PFAM
PF03070	TENA_THI-4	Thiaminase-2/PQQC	PFAM
cd01169	HMPP_kinase	HMP/HMP-P_kinase_dom	CDD
G3DSA:3.40.1190.20:FF:000096			FUNFAM
SSF48613	Heme oxygenase-like	Haem_Oase-like_multi-hlx	SUPERFAMILY
SSF53613	Ribokinase-like	Ribokinase-like	SUPERFAMILY
G3DSA:3.40.1190.20		Ribokinase-like	GENE3D
G3DSA:1.20.910.10		Haem_Oase-like_multi-hlx	GENE3D
PTHR20858	PHOSPHOMETHYLPYRIMIDINE KINASE		PANTHER
TIGR00097	HMP-P_kinase	HMP/HMP-P_kinase_dom	NCBIFAM

Orthologs

thi20 (SJAG_02421) Schizosaccharomyces japonicus
THI20 (YOL055C) Saccharomyces cerevisiae
THI21 (YPL258C) Saccharomyces cerevisiae
THI22 (YPR121W) Saccharomyces cerevisiae

References / Literature

PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.: Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000041 - Gene Ontology annotation based on UniPathway vocabulary mapping.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053