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protein coding gene - thi20 (SPBP8B7.17c) - phosphomethylpyrimidine kinase Thi20

Gene summary

Standard name
thi20
Systematic ID
SPBP8B7.17c
Product
phosphomethylpyrimidine kinase Thi20
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94265
ORFeome ID
24/24F08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3665670..3667739 reverse strand

Annotation

GO biological process

GO:0009228 - thiamine biosynthetic process

References:

GO:0009229 - thiamine diphosphate biosynthetic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0008902 - hydroxymethylpyrimidine kinase activity

References:

GO:0008972 - phosphomethylpyrimidine kinase activity

References:

Genome organisation

PBO:0000758 - tandem duplication

Multi-locus phenotype

FYPO:0002243 - increased acid phosphatase activity

References:

Genotypes:

FYPO:0003267 - normal acid phosphatase activity

References:

Genotypes:

Protein features

PBO:0111917 - TENA/THI domain

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0002243 - increased acid phosphatase activity

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF03070TENA_THI-4Thiaminase-2/PQQCPfam
PF08543Phos_pyr_kinPM/HMP-P_kinase-1Pfam
cd01169HMPP_kinaseHMP/HMP-P_kinase_domCDD
G3DSA:3.40.1190.20:FF:000096CATH-FunFam
G3DSA:1.20.910.10Haem_Oase-like_multi-hlxCATH-Gene3D
G3DSA:3.40.1190.20Ribokinase-likeCATH-Gene3D
SSF48613Haem_Oase-like_multi-hlxSUPERFAMILY
SSF53613Ribokinase-likeSUPERFAMILY
PTHR20858PANTHER
TIGR00097HMP-P_kinaseHMP/HMP-P_kinase_domNCBIFAM

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000041 - Gene Ontology annotation based on UniPathway vocabulary mapping.
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83