PomBase home

protein coding gene - mug30 (SPBP8B7.27) - HECT-type ubiquitin-protein ligase E3, found in association with TRAMP complex

Gene summary

Standard name
mug30
Systematic ID
SPBP8B7.27
Product
HECT-type ubiquitin-protein ligase E3, found in association with TRAMP complex
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94275
ORFeome ID
28/28H03
Characterisation status
conserved unknown
Feature type
mRNA gene
Genomic location
chromosome II: 3688539..3691849 forward strand

Annotation

GO cellular component

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0061630 - ubiquitin protein ligase activity

References:

GO:0008270 - zinc ion binding

References:

Protein features

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111784 - zf-UBP type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000829 - resistance to cisplatin

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0009060 - resistance to wortmannin

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00632HECTHECT_domPFAM
cd00078HECTcHECT_domCDD
PS50237HECTHECT_domPROSITE_PROFILES
SM00119hect_3HECT_domSMART
SM00290Zf_UBP_1Znf_UBPSMART
G3DSA:3.30.2160.10:FF:000004FUNFAM
SSF56204Hect, E3 ligase catalytic domainHect_E3_ubiquitin_ligaseSUPERFAMILY
G3DSA:3.90.1750.10Hect, E3 ligase catalytic domainsGENE3D
G3DSA:3.30.2160.10Hect, E3 ligase catalytic domainGENE3D
G3DSA:3.30.2410.10Hect, E3 ligase catalytic domainGENE3D
PTHR45700UBIQUITIN-PROTEIN LIGASE E3CE3A/B/C-likePANTHER

Orthologs

References / Literature

PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:31657618 - A Genome-Wide Screen for Wortmannin-Resistant Mutants in Schizosaccharomyces pombe: The Phosphorylation-Impaired Mutants Are Resistant to Signaling Defect.
Yılmazer M et al. DNA Cell Biol 2019 Dec;38(12):1427-1436
GO_REF:0000002 - Comments
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021