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protein coding gene - thi5 (SPBP8B7.30c) - DNA-binding transcription factor Thi5

Gene summary

Standard name
thi5
Systematic ID
SPBP8B7.30c
Product
DNA-binding transcription factor Thi5
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94278
ORFeome ID
38/38D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3694964..3697705 reverse strand

Annotation

GO biological process

GO:0090180 - positive regulation of thiamine biosynthetic process

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0016020 - membrane

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

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GO:0008270 - zinc ion binding

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Miscellaneous functional group

PBO:0002382 - membrane-tethered transcription factor

References:

Protein features

PBO:0111744 - zf-fungal Zn(2)-Cys(6) binuclear cluster domain

PBO:0111743 - zinc finger protein

Protein sequence feature

SO:0001528 - nuclear_localization_signal

References:

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003796 - abolished transcription from RNA polymerase II promoter

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Genotypes:

FYPO:0000046 - decreased cell population growth

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0009076 - increased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0000761 - increased conjugation frequency

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0003797 - normal conjugation frequency

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00172Zn_clusZn2Cys6_DnaBDPFAM
PF04082Fungal_transXnlR_reg_domPFAM
cd12148fungal_TF_MHRCDD
cd00067GAL4Zn2Cys6_DnaBDCDD
PS00463ZN2_CY6_FUNGAL_1Zn2Cys6_DnaBDPROSITE_PATTERNS
PS50048ZN2_CY6_FUNGAL_2Zn2Cys6_DnaBDPROSITE_PROFILES
SM00066gal4_2Zn2Cys6_DnaBDSMART
G3DSA:4.10.240.10:FF:000028FUNFAM
SSF57701Zn2/Cys6 DNA-binding domainZn2-C6_fun-type_DNA-bd_sfSUPERFAMILY
G3DSA:4.10.240.10Zn2-C6_fun-type_DNA-bd_sfGENE3D
PTHR47540THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:11790253 - Computational prediction of membrane-tethered transcription factors.
Zupicich J et al. Genome Biol 2001;2(12):RESEARCH0050
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16874521 - Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe.
McQuire TA et al. Curr Genet 2006 Oct;50(4):269-79
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83