PomBase home

protein coding gene - gpt1 (SPBPJ4664.06) - UDP-glucose-glycoprotein glucosyltransferase Gpt1

Gene summary

Standard name
gpt1
Systematic ID
SPBPJ4664.06
Product
UDP-glucose-glycoprotein glucosyltransferase Gpt1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09140
ORFeome ID
49/49H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 707514..712426 forward strand

Annotation

PBO:0000169 - 2.4.1.-

Disease association

MONDO:0015286 - congenital disorder of glycosylation

References:

MONDO:0980705 - congenital disorder of glycosylation, type IIcc

References:

GO biological process

GO:0006491 - N-glycan processing

References:

GO cellular component

GO:0005788 - endoplasmic reticulum lumen

References:

GO molecular function

GO:0005509 - calcium ion binding

References:

GO:0005537 - D-mannose binding

References:

GO:0051787 - misfolded protein binding

References:

GO:0003980 - UDP-glucose:glycoprotein glucosyltransferase activity

References:

Modification

MOD:00595 - monomannosylated residue

References:

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0005760 - increased cell-cell adhesion

References:

Genotypes:

FYPO:0003333 - inviable lemon-shaped cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001806 - ER_retention_signal

References:

SO:0000418 - signal_peptide

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0001456 - increased RNA level during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF06427UDP-g_GGTaseUDP-g_GGtransPfam
PF18400Thioredoxin_12UGGT_TRXL_1Pfam
PF18401Thioredoxin_13UGGT_TRXL_2Pfam
PF18402Thioredoxin_14UGGT_TRXL_3Pfam
PF18403Thioredoxin_15UGGT_TRXL_4Pfam
PF18404Glyco_transf_24Glyco_transf_24Pfam
cd06432GT8_HUGT1_C_likeCDD
G3DSA:3.90.550.10:FF:000065CATH-FunFam
G3DSA:3.90.550.10Nucleotide-diphossugar_transCATH-Gene3D
SSF53448Nucleotide-diphossugar_transSUPERFAMILY
PTHR11226UDP-g_GGtransPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9813085 - The UDP-Glc:Glycoprotein glucosyltransferase is essential for Schizosaccharomyces pombe viability under conditions of extreme endoplasmic reticulum stress.
Fanchiotti S et al. J Cell Biol 1998 Nov 02;143(3):625-35
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:9860839 - Calnexin and BiP interact with acid phosphatase independently of glucose trimming and reglucosylation in Schizosaccharomyces pombe.
Jannatipour M et al. Biochemistry 1998 Dec 08;37(49):17253-61
PMID:22682253 - Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A et al. Cell 2012 Jun 08;149(6):1339-52
PB_REF:0000003 - Disease Association Curation
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:7982990 - Purification to homogeneity of UDP-glucose:glycoprotein glucosyltransferase from Schizosaccharomyces pombe and apparent absence of the enzyme fro Saccharomyces cerevisiae.
Fernández FS et al. J Biol Chem 1994 Dec 02;269(48):30701-6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:10198054 - Uridine diphosphate-glucose transport into the endoplasmic reticulum of Saccharomyces cerevisiae: in vivo and in vitro evidence.
Castro O et al. Mol Biol Cell 1999 Apr;10(4):1019-30
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:27587357 - The conundrum of UDP-Glc entrance into the yeast ER lumen.
Bredeston LM et al. Glycobiology 2017 Jan;27(1):64-79
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:8631292 - A new stress protein: synthesis of Schizosaccharomyces pombe UDP--Glc:glycoprotein glucosyltransferase mRNA is induced by stress conditions but the enzyme is not essential for cell viability.
Fernandez F et al. EMBO J 1996 Feb 15;15(4):705-13
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83