protein coding gene - spc7 (SPCC1020.02) - outer kinetochore KNL network protein, KNL-1 family member, blinkin, Spc7

Gene summary

Standard name

spc7

Systematic ID

SPCC1020.02

Product

outer kinetochore KNL network protein, KNL-1 family member, blinkin, Spc7

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O59757

ORFeome ID

30/30F05

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 786289..790660 reverse strand

Annotation

Protein features

Orthologs

• KNL1 (HGNC:24054) Homo sapiens
• spc7 (SJAG_04259) Schizosaccharomyces japonicus
• SPC105 (YGL093W) Saccharomyces cerevisiae

References / Literature

PMID:23512483 - Making an effective switch at the kinetochore by phosphorylation and dephosphorylation.

Funabiki H et al. Chromosoma 2013 Jun;122(3):135-58

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.

Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54

PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.

Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140

PMID:27618268 - Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.

Mora-Santos MD et al. Curr Biol 2016 Oct 10;26(19):2642-2650

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.

Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:29196561 - Hierarchical Regulation of Centromeric Cohesion Protection by Meikin and Shugoshin during Meiosis I.

Miyazaki S et al. Cold Spring Harb Symp Quant Biol 2017;82:259-266

PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.

Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567

PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.

Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67

PMID:28017606 - Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.

Yuan I et al. Curr Biol 2017 Jan 09;27(1):137-143

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:15502821 - A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.

Obuse C et al. Nat Cell Biol 2004 Nov;6(11):1135-41

PMID:34849791 - Perturbation of kinetochore function using GFP-binding protein in fission yeast.

Deng DJ et al. G3 (Bethesda) 2021 Oct 19;11(11)

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.

Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78

PMID:21664573 - Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.

Meadows JC et al. Dev Cell 2011 Jun 14;20(6):739-50

PMID:22660415 - MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.

Yamagishi Y et al. Nat Cell Biol 2012 Jun 03;14(7):746-52

GO_REF:0000051 - S. pombe keyword mapping

PMID:22065639 - Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation.

Chen JS et al. J Cell Biol 2011 Nov 14;195(4):583-93

PMID:29180432 - Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.

Zhou X et al. Proc Natl Acad Sci U S A 2017 Dec 12;114(50):E10667-E10676

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.

Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308

PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.

Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7

PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.

Jian Y et al. J Cell Sci 2023 Jan 15;136(2)

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.

Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84

PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.

Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:22711988 - Sos7, an essential component of the conserved Schizosaccharomyces pombe Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore proteins.

Jakopec V et al. Mol Cell Biol 2012 Aug;32(16):3308-20

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.

Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:36705602 - Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.

Virant D et al. J Cell Biol 2023 Apr 03;222(4)