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protein coding gene - gpi8 (SPCC11E10.02c) - GPI-anchor transamidase complex catalytic subunit Pig-K, Gpi8

Gene summary

Standard name
gpi8
Systematic ID
SPCC11E10.02c
Product
GPI-anchor transamidase complex catalytic subunit Pig-K, Gpi8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9USP5
ORFeome ID
18/18A12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1454541..1456159 reverse strand

Annotation

PBO:0005508 - 3.4.17.2

Complementation

PBO:0005507 - functionally complements S. cerevisiae GPI8

References:

Disease association

MONDO:0030037 - neurodevelopmental disorder with hypotonia and cerebellar atrophy, with or without seizures

References:

GO biological process

GO:0016255 - attachment of GPI anchor to protein

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0042765 - GPI-anchor transamidase complex

References:

GO molecular function

GO:0003923 - GPI-anchor transamidase activity

References:

Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00006 - N-glycosylated residue

References:

Protein sequence feature

SO:0000418 - signal_peptide

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01650Peptidase_C13Peptidase_C13Pfam
PR00776HEMOGLOBNASEPeptidase_C13PRINTS
G3DSA:3.40.50.1460:FF:000021CATH-FunFam
G3DSA:3.40.50.1460CATH-Gene3D
PTHR48067GPI_transamidasePANTHER
PIRSF019663LegumainPeptidase_C13PIRSF
PIRSF500138GPI8GPI_transamidasePIRSF
Transmembrane alpha helixDeepTMHMM
Signal PeptideDeepTMHMM-Signal-Peptide

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:11124699 - The Schizosaccharomyces pombe GPI8 gene complements a Saccharomyces cerevisiae GPI8 anchoring mutant.
Shams-Eldin H et al. Yeast 2001 Jan 15;18(1):33-9
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37792890 - Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.
Willet AH et al. PLoS Genet 2023 Oct;19(10):e1010987