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protein coding gene - nmt1 (SPCC1223.02) - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1

Gene summary

Standard name
nmt1
Systematic ID
SPCC1223.02
Product
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
thi3
UniProt ID
P36597
ORFeome ID
15/15G09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1838415..1839664 forward strand

Annotation

GO biological process

GO:0009228 - thiamine biosynthetic process

References:

GO:0009229 - thiamine diphosphate biosynthetic process

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0106344 - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP

References:

Modification

MOD:00128 - N6-pyridoxal phosphate-L-lysine

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000015 - RNA absent

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0001907 - growth auxotrophic for thiamine

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF09084NMT1SsuA/THI5Pfam
cd13650PBP2_THI5CDD
G3DSA:3.40.190.10:FF:000187CATH-FunFam
G3DSA:3.40.190.10CATH-Gene3D
SSF53850SUPERFAMILY
PTHR31528NMT1/THI5PANTHER

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:8675019 - Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-repressible gene car1.
Niederberger C et al. Gene 1996 May 24;171(1):119-22
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:2358444 - nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
Maundrell K J Biol Chem 1990 Jul 05;265(19):10857-64
PMID:8163491 - ntf1+ encodes a 6-cysteine zinc finger-containing transcription factor that regulates the nmt1 promoter in fission yeast.
Tang CS et al. J Biol Chem 1994 Apr 22;269(16):11921-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:25102102 - A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast.
Kim JY et al. Aging (Albany NY) 2014 Jul;6(7):587-601
PMID:21828039 - Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Beaudoin J et al. J Biol Chem 2011 Sep 30;286(39):34356-72
GO_REF:0000041 - Gene Ontology annotation based on UniPathway vocabulary mapping.
PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.
Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16874521 - Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe.
McQuire TA et al. Curr Genet 2006 Oct;50(4):269-79
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:25117315 - A potential protective role for thiamine in glucose-driven oxidative stress.
Palabiyik B et al. Genet Mol Res 2014 Jul 25;13(3):5582-93
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:15837798 - Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.
Janson ME et al. J Cell Biol 2005 Apr 25;169(2):297-308
PMID:1868574 - Thiamine in Schizosaccharomyces pombe: dephosphorylation, intracellular pool, biosynthesis and transport.
Schweingruber AM et al. Curr Genet 1991 Apr;19(4):249-54
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7