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protein coding gene - cbf12 (SPCC1223.13) - DNA-binding transcription factor, CBF1/Su(H)/LAG-1 family Cbf12

Gene summary

Standard name
cbf12
Systematic ID
SPCC1223.13
Product
DNA-binding transcription factor, CBF1/Su(H)/LAG-1 family Cbf12
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74412
ORFeome ID
47/47D06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1871367..1875842 forward strand

Annotation

Comment

PBO:0034732 - chromatin binding profiling

References:

PBO:0034781 - WT overexpression expression profiling

References:

Disease association

MONDO:0013895 - Adams-Oliver syndrome 3

References:

GO biological process

GO:1900735 - positive regulation of flocculation

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

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Genotypes:

FYPO:0000117 - abnormal septum assembly

References:

Genotypes:

FYPO:0000010 - abolished cell-substrate adhesion

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Genotypes:

FYPO:0000659 - abolished DNA binding

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Genotypes:

FYPO:0005504 - abolished galactose-specific flocculation

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Genotypes:

FYPO:0002779 - abolished protein localization to nucleus, with protein mislocalized to cytoplasm, during vegetative growth

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Genotypes:

FYPO:0004363 - altered level of stress responsive gene mRNA during vegetative growth

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

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Genotypes:

FYPO:0008249 - decreased protein localization to chromatin at protein coding gene

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Genotypes:

FYPO:0004056 - decreased protein localization to nucleus, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0000657 - increased DNA binding

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Genotypes:

FYPO:0000155 - increased flocculation

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Genotypes:

FYPO:0005505 - increased level of cell surface glycoprotein gene mRNA during vegetative growth

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0000252 - increased spontaneous diploidization

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

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Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0001903 - normal septation index

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007474 - variable cell size at division

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF09270BTDBeta-trefoil_DNA-bd_domPfam
PF09271LAG1-DNAbindRBP-J/Cbf11/Cbf12_DNA-bdPfam
SM01267LAG1_DNAbindRBP-J/Cbf11/Cbf12_DNA-bdSMART
SM01268BTDBeta-trefoil_DNA-bd_domSMART
G3DSA:2.80.10.50CATH-Gene3D
SSF110217BTD_sfSUPERFAMILY
SSF49417p53-like_TF_DNA-bd_sfSUPERFAMILY
PTHR10665CLS_famPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:21858190 - N-termini of fungal CSL transcription factors are disordered, enriched in regulatory motifs and inhibit DNA binding in fission yeast.
Převorovský M et al. PLoS One 2011;6(8):e23650
PMID:26366556 - Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.
Převorovský M et al. PLoS One 2015;10(9):e0137820
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.
Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)
PMID:26896331 - Conserved and Diverged Functions of the Calcineurin-Activated Prz1 Transcription Factor in Fission Yeast.
Chatfield-Reed K et al. Genetics 2016 Apr;202(4):1365-75
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:23555033 - Fission yeast CSL proteins function as transcription factors.
Oravcová M et al. PLoS One 2013;8(3):e59435
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:36794724 - Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis.
Garg A et al. Nucleic Acids Res 2023 Apr 24;51(7):3094-3115
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:36820394 - Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast.
Sanchez AM et al. MicroPubl Biol 2023;2023
PMID:19101542 - Cbf11 and Cbf12, the fission yeast CSL proteins, play opposing roles in cell adhesion and coordination of cell and nuclear division.
Prevorovský M et al. Exp Cell Res 2009 May 01;315(8):1533-47
PMID:23236291 - Deciphering the transcriptional-regulatory network of flocculation in Schizosaccharomyces pombe.
Kwon EJ et al. PLoS Genet 2012;8(12):e1003104
PMID:38482739 - Critical importance of DNA binding for CSL protein functions in fission yeast.
Marešová A et al. J Cell Sci 2024 Mar 14;
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514