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protein coding gene - sgf73 (SPCC126.04c) - SAGA complex deubiquitinating submodule subunit Sgf73

Gene summary

Standard name
sgf73
Systematic ID
SPCC126.04c
Product
SAGA complex deubiquitinating submodule subunit Sgf73
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94397
ORFeome ID
17/17D04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 2122510..2123930 reverse strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

Disease association

MONDO:0005071 - nervous system disorder

References:

MONDO:0016163 - spinocerebellar ataxia 7

References:

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:1904802 - RITS complex assembly

References:

GO:0140727 - siRNA-mediated pericentric heterochromatin formation

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GO:0045815 - transcription initiation-coupled chromatin remodeling

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0071819 - DUBm complex

References:

GO:0005634 - nucleus

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GO:0000124 - SAGA complex

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GO molecular function

GO:0004843 - cysteine-type deubiquitinase activity

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GO:0005515 - protein binding

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GO:0003713 - transcription coactivator activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002398 - abnormal actin cytoskeleton during vegetative growth

References:

Genotypes:

FYPO:0000164 - abnormal cell separation after cytokinesis

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Genotypes:

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0005053 - abnormal RITS complex assembly

References:

Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0000888 - decreased histone H3-K9 dimethylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001253 - elongated multinucleate multiseptate vegetative cell, single septa between nuclei

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Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0005258 - increased cell population growth at high temperature

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Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

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Genotypes:

FYPO:0003009 - increased protein localization to centromere outer repeat

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0000252 - increased spontaneous diploidization

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Genotypes:

FYPO:0000239 - increased transcription from MCB promoter

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

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Genotypes:

FYPO:0000551 - pseudohyphal growth abolished

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000099 - sensitive to canavanine

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Genotypes:

FYPO:0003384 - sensitive to chromium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08313SCA7SCA7_domPfam
PS51505SCA7SCA7_domPROSITE profiles
G3DSA:6.10.140.1270CATH-Gene3D
PTHR47805SGF73PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
GO_REF:0000051 - S. pombe keyword mapping
PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.
Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29079657 - TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.
Laboucarié T et al. EMBO Rep 2017 Dec;18(12):2197-2218
PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.
Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:25526805 - Uncovering the role of Sgf73 in maintaining SAGA deubiquitinating module structure and activity.
Yan M et al. J Mol Biol 2015 Apr 24;427(8):1765-78
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
Gaspa L et al. Cell Cycle 2016;15(5):720-9
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:22992726 - The SAGA histone acetyltransferase complex regulates leucine uptake through the Agp3 permease in fission yeast.
Takahashi H et al. J Biol Chem 2012 Nov 02;287(45):38158-67
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:24948786 - Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe.
Elmore ZC et al. G3 (Bethesda) 2014 Jun 19;4(8):1529-38
PMID:26443059 - Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast.
Deng X et al. Sci Rep 2015 Oct 07;5:14707
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:19056896 - The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.
Helmlinger D et al. Genes Dev 2008 Nov 15;22(22):3184-95
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:22624651 - A systematic screen reveals new elements acting at the G2/M cell cycle control.
Navarro FJ et al. Genome Biol 2012 May 24;13(5):R36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000003 - Disease Association Curation
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:21642955 - Tra1 has specific regulatory roles, rather than global functions, within the SAGA co-activator complex.
Helmlinger D et al. EMBO J 2011 Jun 03;30(14):2843-52
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:19542312 - Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.
Dodgson J et al. Eukaryot Cell 2009 Aug;8(8):1298-306
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:31748520 - Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast.
Elías-Villalobos A et al. Nat Commun 2019 Nov 20;10(1):5237
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6