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protein coding gene - ags1 (SPCC1281.01) - cell wall alpha-1,3-glucan synthase Ags1

Gene summary

Standard name
ags1
Systematic ID
SPCC1281.01
Product
cell wall alpha-1,3-glucan synthase Ags1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mok1, SPCC338.01c, SPCC17A7.01, tps16
UniProt ID
Q9USK8
ORFeome ID
48/48H12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1373097..1381763 forward strand

Annotation

PBO:0000169 - 2.4.1.-

PBO:0001601 - 3.2.1.1

GO biological process

GO:0030979 - alpha-glucan biosynthetic process

References:

GO:0070600 - fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO:0009272 - fungal-type cell wall biogenesis

References:

GO:0031671 - primary cell septum biogenesis

References:

GO cellular component

GO:0005619 - ascospore wall

References:

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0000935 - division septum

References:

GO:0009897 - external side of plasma membrane

References:

GO:0032178 - medial membrane band

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GO:0016020 - membrane

References:

GO:0035841 - new growing cell tip

References:

GO:0035840 - old growing cell tip

References:

GO:0005886 - plasma membrane

References:

GO:0031520 - plasma membrane of cell tip

References:

GO molecular function

GO:0047657 - alpha-1,3-glucan synthase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001235 - decreased extent of cell population growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0007293 - lysed spherical vegetative cell

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001315 - normal vegetative cell morphology

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000174 - abnormal cell wall biogenesis

References:

Genotypes:

FYPO:0003211 - abnormal secondary cell septum biogenesis

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0001081 - decreased cell wall alpha-glucan level

References:

Genotypes:

FYPO:0002265 - decreased cell wall galactomannan level

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0006802 - dispersed filamentous actin

References:

Genotypes:

FYPO:0003213 - explosive cytokinetic cell separation resulting in vegetative cell lysis

References:

Genotypes:

FYPO:0001084 - increased cell wall alpha-glucan level

References:

Genotypes:

FYPO:0001035 - increased cell wall thickness during vegetative growth

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002630 - irregular cell wall during vegetative growth

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Genotypes:

FYPO:0003212 - kinked septum

References:

Genotypes:

FYPO:0003795 - normal cell wall alpha-glucan level

References:

Genotypes:

FYPO:0007114 - normal microtubule bundle

References:

Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0007294 - pear-shaped vegetative cell with actin localized to rounded end

References:

Genotypes:

FYPO:0007359 - resistance to heavy water

References:

Genotypes:

FYPO:0002720 - sensitive to beta-glucanase

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0000113 - sensitive to staurosporine

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0007230 - spindle-shaped cell

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0004103 - viable spherical vegetative cell

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002104 - viable vegetative cell with normal cell shape

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF26114Ig_2_Mok13Mok11-13/Ags1-like_Ig_2PFAM
PF00534Glycos_transf_1Glyco_trans_1PFAM
PF26108GH_Mok13Mok11-13/Ags1-like_GHPFAM
PF00128Alpha-amylaseGH13_cat_domPFAM
PF26111Ig_Mok13Mok11-13/Ags1-like_IgPFAM
PF08323Glyco_transf_5Starch_synth_cat_domPFAM
PF26122CBM_Mok13Mok11-13/Ags1-like_CBMPFAM
PF2612712TM_Mok13Mok11-14/Ags1-like_TMPFAM
cd03791GT5_Glycogen_synthase_DULL1-likeCDD
cd11323AmyAc_AGSCDD
cd06174MFSCDD
SM00642aamyGH13_cat_domSMART
G3DSA:3.40.50.2000:FF:000052FUNFAM
G3DSA:3.40.50.2000:FF:000058FUNFAM
G3DSA:3.20.20.80:FF:000073FUNFAM
SSF53756UDP-Glycosyltransferase/glycogen phosphorylaseSUPERFAMILY
SSF51445(Trans)glycosidasesGHSUPERFAMILY
G3DSA:3.40.50.2000Glycogen Phosphorylase B;GENE3D
G3DSA:3.20.20.80GlycosidasesGENE3D
PTHR47182CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATEDMok11-14/Ags1-likePANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity

Orthologs

References / Literature

PMID:10087262 - Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.
Katayama S et al. J Cell Biol 1999 Mar 22;144(6):1173-86
PMID:23934882 - Negative functional interaction between cell integrity MAPK pathway and Rho1 GTPase in fission yeast.
Viana RA et al. Genetics 2013 Oct;195(2):421-32
PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.
Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.
Ramos M et al. iScience 2024 Aug 16;27(8):110477
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:40668835 - α-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.
Jacob A et al. Proc Natl Acad Sci U S A 2025 Jul 22;122(29):e2505509122
PMID:37792890 - Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.
Willet AH et al. PLoS Genet 2023 Oct;19(10):e1010987
PMID:27385337 - Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
Liu Y et al. Mol Biol Cell 2016 Aug 15;27(16):2528-41
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22891259 - Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission.
Cortés JC et al. J Cell Biol 2012 Aug 20;198(4):637-56
PMID:41144523 - The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.
Zhang S et al. PLoS Biol 2025 Oct 27;23(10):e3003466
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:17472966 - Role of the synthase domain of Ags1p in cell wall alpha-glucan biosynthesis in fission yeast.
Vos A et al. J Biol Chem 2007 Jun 29;282(26):18969-79
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:12206652 - Mac1, a fission yeast transmembrane protein localizing to the poles and septum, is required for correct cell separation at high temperatures.
Grandin N et al. Biol Cell 2002 Jun;94(3):127-37
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.
Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:12673626 - Characterization and behaviour of alpha-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy.
Konomi M et al. Yeast 2003 Apr 15;20(5):427-38
PMID:16420355 - Synthesis of alpha-glucans in fission yeast spores is carried out by three alpha-glucan synthase paralogues, Mok12p, Mok13p and Mok14p.
García I et al. Mol Microbiol 2006 Feb;59(3):836-53
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:30332655 - Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast.
Martín-García R et al. Cell Rep 2018 Oct 16;25(3):772-783.e4
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.
Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:9689051 - Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast.
Hochstenbach F et al. Proc Natl Acad Sci U S A 1998 Aug 04;95(16):9161-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:11112691 - Two type V myosins with non-overlapping functions in the fission yeast Schizosaccharomyces pombe: Myo52 is concerned with growth polarity and cytokinesis, Myo51 is a component of the cytokinetic actin ring.
Win TZ et al. J Cell Sci 2001 Jan;114(Pt 1):69-79
PMID:34910579 - Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.
Longo LVG et al. Mol Biol Cell 2022 Feb 01;33(2):ar17
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:26132084 - Cooperation between Paxillin-like Protein Pxl1 and Glucan Synthase Bgs1 Is Essential for Actomyosin Ring Stability and Septum Formation in Fission Yeast.
Cortés JC et al. PLoS Genet 2015 Jul;11(7):e1005358
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:38442865 - The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.
Kume K et al. Open Biol 2024 Mar;14(3):230440
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6