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protein coding gene - lap2 (SPCC1322.05c) - vacuolar aminopeptidase Lap2

Gene summary

Standard name
lap2
Systematic ID
SPCC1322.05c
Product
vacuolar aminopeptidase Lap2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppp85
UniProt ID
O94544
ORFeome ID
36/36G11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1293804..1296128 reverse strand

Annotation

GO biological process

GO:0120113 - cytoplasm to vacuole targeting by the NVT pathway

References:

GO:0043171 - peptide catabolic process

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GO:0030163 - protein catabolic process

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0000328 - fungal-type vacuole lumen

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GO:0005771 - multivesicular body

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GO:0005634 - nucleus

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GO:0061957 - NVT complex

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GO molecular function

GO:0004301 - epoxide hydrolase activity

References:

GO:0070006 - metalloaminopeptidase activity

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GO:0008270 - zinc ion binding

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Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006274 - abolished protein localization via NVT pathway

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0006294 - normal macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0009060 - resistance to wortmannin

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01433Peptidase_M1Peptidase_M1_domPfam
PF09127Leuk-A4-hydro_CPeptidase_M1_CPfam
PF17900Peptidase_M1_NAminopeptidase_N-like_NPfam
cd09599M1_LTA4HLTA4H_catCDD
SM01263Leuk-A4-hydro_CPeptidase_M1_CSMART
PR00756ALADIPTASEPeptidase_M1PRINTS
G3DSA:1.10.390.10:FF:000009CATH-FunFam
G3DSA:1.25.40.320:FF:000001CATH-FunFam
G3DSA:2.60.40.1730:FF:000004CATH-FunFam
G3DSA:3.30.2010.30:FF:000001CATH-FunFam
G3DSA:1.10.390.10Peptidase_M4/M1_CTD_sfCATH-Gene3D
G3DSA:1.25.40.320M1_LTA-4_hydro/amino_C_sfCATH-Gene3D
G3DSA:2.60.40.1730Aminopeptidase_N-like_N_sfCATH-Gene3D
G3DSA:3.30.2010.30CATH-Gene3D
SSF48371ARM-type_foldSUPERFAMILY
SSF55486SUPERFAMILY
SSF63737Aminopeptidase_N-like_N_sfSUPERFAMILY
PTHR45726M1_LTA4HPANTHER
TIGR02411leuko_A4_hydroLTA4HNCBIFAM

Orthologs

References / Literature

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:34169534 - Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.
Wang YY et al. EMBO J 2021 Aug 02;40(15):e107497
PMID:31657618 - A Genome-Wide Screen for Wortmannin-Resistant Mutants in Schizosaccharomyces pombe: The Phosphorylation-Impaired Mutants Are Resistant to Signaling Defect.
Yılmazer M et al. DNA Cell Biol 2019 Dec;38(12):1427-1436
PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.
Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12