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protein coding gene - bub1 (SPCC1322.12c) - mitotic spindle checkpoint kinase Bub1

Gene summary

Standard name
bub1
Systematic ID
SPCC1322.12c
Product
mitotic spindle checkpoint kinase Bub1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94751
ORFeome ID
30/30A12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1312560..1315997 reverse strand

Annotation

Disease association

MONDO:0005575 - colorectal cancer

References:

MONDO:0859342 - microcephaly 30, primary, autosomal recessive

References:

MONDO:0009759 - mosaic variegated aneuploidy syndrome 1

References:

MONDO:0008304 - premature chromatid separation trait

References:

GO biological process

GO:0006338 - chromatin remodeling

References:

GO:1905318 - meiosis I spindle assembly checkpoint signaling

References:

GO:1990813 - meiotic centromeric cohesion protection in anaphase I

References:

GO:1990758 - mitotic sister chromatid biorientation

References:

GO:0007094 - mitotic spindle assembly checkpoint signaling

References:

GO cellular component

GO:1990298 - bub1-bub3 complex

References:

GO:0034506 - chromosome, centromeric core domain

References:

GO:0000775 - chromosome, centromeric region

References:

GO:0000776 - kinetochore

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005634 - nucleus

References:

GO:0071957 - old mitotic spindle pole body

References:

GO:0000940 - outer kinetochore

References:

GO molecular function

GO:0072371 - histone H2AS121 kinase activity

References:

GO:0004674 - protein serine/threonine kinase activity

References:

GO:0035591 - signaling adaptor activity

References:

Modification

MOD:00042 - L-aspartic 4-phosphoric anhydride

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0005045 - abnormal mitotic sister chromatid segregation with lagging chromosomes and decreased rate of mitotic spindle elongation

References:

Genotypes:

FYPO:0000168 - abnormal mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0008164 - abnormal protein localization to kinetochore during mitotic M phase

References:

Genotypes:

FYPO:0007745 - abnormal spindle assembly during meiosis I

References:

Genotypes:

FYPO:0004318 - abolished mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0005781 - decreased duration of mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0006423 - decreased meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0005300 - decreased protein localization to kinetochore during mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0002825 - decreased protein localization to mitotic spindle

References:

Genotypes:

FYPO:0005780 - decreased response to mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0008202 - equational sister chromatid separation at meiosis I

References:

Genotypes:

FYPO:0002638 - increased activation of mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0005684 - increased duration of mitotic prometaphase

References:

Genotypes:

FYPO:0005683 - increased duration of mitotic prophase

References:

Genotypes:

FYPO:0007856 - increased duration of protein localization to spindle during meiosis I

References:

Genotypes:

FYPO:0002059 - inviable cell population

References:

Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0001489 - inviable vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0004827 - inviable vegetative cell with cell death during mitosis

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0005510 - meiosis II metaphase/anaphase transition delay

References:

Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

References:

Genotypes:

FYPO:0005383 - normal duration of meiosis I

References:

Genotypes:

FYPO:0006646 - normal duration of mitotic prometaphase

References:

Genotypes:

FYPO:0006257 - normal duration of mitotic prophase

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0003762 - normal mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0006917 - normal onset of mitotic metaphase/anaphase transition

References:

Genotypes:

FYPO:0007399 - normal protein localization to kinetochore during mitotic interphase

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003182 - sister chromatid nondisjunction at meiosis II

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0006821 - slow vegetative cell growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0000326 - abnormal mitotic sister chromatid biorientation

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0008164 - abnormal protein localization to kinetochore during mitotic M phase

References:

Genotypes:

FYPO:0010054 - abolished histone H2A-S121 phosphorylation at subtelomere during vegetative growth

References:

Genotypes:

FYPO:0006424 - abolished meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0004318 - abolished mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0001384 - abolished protein kinase activity

References:

Genotypes:

FYPO:0006457 - abolished protein localization to centromere central core during meiosis I

References:

Genotypes:

FYPO:0004763 - abolished protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0007663 - abolished protein localization to kinetochore during meiosis II

References:

Genotypes:

FYPO:0007662 - abolished protein localization to kinetochore during meiotic anaphase I

References:

Genotypes:

FYPO:0007688 - abolished protein localization to kinetochore during meiotic metaphase I

References:

Genotypes:

FYPO:0007400 - abolished protein localization to kinetochore during mitotic interphase

References:

Genotypes:

FYPO:0008166 - abolished protein localization to kinetochore during mitotic prometaphase

References:

Genotypes:

FYPO:0007383 - abolished protein localization to kinetochore during mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0006455 - abolished protein localization to meiotic spindle during meiosis I

References:

Genotypes:

FYPO:0006456 - abolished protein localization to meiotic spindle during meiosis II

References:

Genotypes:

FYPO:0007650 - abolished protein localization to pericentric heterochromatin during meiotic prophase I

References:

Genotypes:

FYPO:0007651 - abolished protein localization to pericentric heterochromatin during mitosis

References:

Genotypes:

FYPO:0005396 - abolished protein localization to subtelomeric heterochromatin

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0006260 - abolished subtelomeric chromatin knob formation

References:

Genotypes:

FYPO:0000227 - chromosome loss during mitotic chromosome segregation

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002577 - decreased chromatin binding

References:

Genotypes:

FYPO:0006423 - decreased meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0007244 - decreased protein localization to kinetochore during mitotic metaphase

References:

Genotypes:

FYPO:0005300 - decreased protein localization to kinetochore during mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0002825 - decreased protein localization to mitotic spindle

References:

Genotypes:

FYPO:0010051 - decreased protein localization to subtelomeric chromatin knob during vegetative growth

References:

Genotypes:

FYPO:0005918 - decreased protein localization to subtelomeric heterochromatin

References:

Genotypes:

FYPO:0002679 - decreased protein phosphorylation

References:

Genotypes:

FYPO:0007855 - decreased protein phosphorylation during mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0005780 - decreased response to mitotic spindle assembly checkpoint signaling

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0008202 - equational sister chromatid separation at meiosis I

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0006460 - increased histone H2A phosphorylation at protein coding gene during meiosis I

References:

Genotypes:

FYPO:0006462 - increased histone H2A phosphorylation at silent mating-type cassette during meiosis I

References:

Genotypes:

FYPO:0006461 - increased histone H2A phosphorylation at telomere during meiosis I

References:

Genotypes:

FYPO:0010056 - increased histone H3-K36 trimethylation at subtelomere during vegetative growth

References:

Genotypes:

FYPO:0006263 - increased histone H3-K36 trimethylation during vegetative growth

References:

Genotypes:

FYPO:0005225 - increased histone H3-K4 dimethylation during vegetative growth

References:

Genotypes:

FYPO:0010053 - increased histone H4-K12 acetylation at subtelomeric chromatin knob during vegetative growth

References:

Genotypes:

FYPO:0010052 - increased histone H4-K5 acetylation at subtelomeric chromatin knob during vegetative growth

References:

Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0000786 - increased plasmid loss

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0005917 - increased subtelomeric heterochromatin RNA level

References:

Genotypes:

FYPO:0004827 - inviable vegetative cell with cell death during mitosis

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007125 - mitotic sister chromatid separation in absence of mitotic spindle

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001687 - normal growth on benomyl

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0005042 - normal protein localization to kinetochore

References:

Genotypes:

FYPO:0004214 - normal protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0007854 - normal protein localization to kinetochore during meiotic anaphase I

References:

Genotypes:

FYPO:0007661 - normal protein localization to kinetochore during meiotic metaphase I

References:

Genotypes:

FYPO:0007399 - normal protein localization to kinetochore during mitotic interphase

References:

Genotypes:

FYPO:0005212 - normal protein localization to kinetochore during mitotic prometaphase

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0006272 - premature mitotic DNA replication initiation from late origin

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0000094 - sensitive to benomyl

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0003182 - sister chromatid nondisjunction at meiosis II

References:

Genotypes:

FYPO:0005648 - sister kinetochore dissociation in meiotic metaphase I

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0005633 - sister kinetochore dissociation in meiotic metaphase I, normal chromosome segregation in meiosis I, and sister chromatid non-disjunction in meiosis II

References:

Genotypes:

FYPO:0005069 - unequal mitotic sister chromatid segregation following normal mitosis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPfam
PF08311Mad3_BUB1_IMad3/Bub1_IPfam
cd13981STKc_Bub1_BubR1CDD
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE profiles
PS51489BUB1_NMad3/Bub1_IPROSITE profiles
SM00220S_TKcProt_kinase_domSMART
SM00777Mad3_BUB1_IMad3/Bub1_ISMART
G3DSA:1.10.510.10CATH-Gene3D
G3DSA:1.25.40.430CATH-Gene3D
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR14030Bub1/Mad3PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:16360688 - Control of Shugoshin function during fission-yeast meiosis.
Vaur S et al. Curr Biol 2005 Dec 20;15(24):2263-70
PMID:19680287 - Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.
Windecker H et al. EMBO Rep 2009 Sep;10(9):1022-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.
Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:18062930 - Functional characterisation of the Schizosaccharomyces pombe homologue of the leukaemia-associated translocation breakpoint binding protein translin and its binding partner, TRAX.
Jaendling A et al. Biochim Biophys Acta 2008 Feb;1783(2):203-13
PMID:14730319 - The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis.
Kitajima TS et al. Nature 2004 Feb 05;427(6974):510-7
PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.
Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23
PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.
Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307
PMID:19965387 - Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin.
Kawashima SA et al. Science 2010 Jan 08;327(5962):172-7
PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.
Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44
PMID:12606573 - Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint.
Yamaguchi S et al. EMBO J 2003 Mar 03;22(5):1075-87
PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.
Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24
PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.
Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22660415 - MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.
Yamagishi Y et al. Nat Cell Biol 2012 Jun 03;14(7):746-52
PMID:26804021 - Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.
Tashiro S et al. Nat Commun 2016 Jan 25;7:10393
PMID:26900649 - The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.
Chmielewska AE et al. Cell Cycle 2016;15(5):740-7
PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.
Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433
PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:28017606 - Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.
Yuan I et al. Curr Biol 2017 Jan 09;27(1):137-143
PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.
Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39
PMID:34738170 - Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe.
Fukunaga T et al. Appl Microbiol Biotechnol 2021 Dec;105(23):8771-8781
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:11861765 - Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest.
Wang SW et al. J Cell Sci 2002 Feb 01;115(Pt 3):587-98
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:19523829 - Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation.
Choi SH et al. Curr Biol 2009 Jun 23;19(12):985-95
PMID:22281223 - Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.
Zich J et al. Curr Biol 2012 Feb 21;22(4):296-301
PMID:12426374 - Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast.
Garcia MA et al. EMBO J 2002 Nov 15;21(22):6015-24
PMID:27798241 - An acetyltransferase-independent function of Eso1 regulates centromere cohesion.
Lin SJ et al. Mol Biol Cell 2016 Dec 15;27(25):4002-4010
PMID:11870212 - Kinesins klp5(+) and klp6(+) are required for normal chromosome movement in mitosis.
West RR et al. J Cell Sci 2002 Mar 01;115(Pt 5):931-40
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:26581324 - Cleavage and polyadenylation factor, Rna14 is an essential protein required for the maintenance of genomic integrity in fission yeast Schizosaccharomyces pombe.
Sonkar A et al. Biochim Biophys Acta 2016 Feb;1863(2):189-97
PMID:17380189 - Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.
Murakami H et al. PLoS One 2007 Mar 21;2(3):e317
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.
Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:12951601 - Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast.
Sato M et al. Nat Cell Biol 2003 Sep;5(9):764-6; author reply 766
PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.
Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220
PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.
Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7
PMID:29769606 - Malonylation of histone H2A at lysine 119 inhibits Bub1-dependent H2A phosphorylation and chromosomal localization of shugoshin proteins.
Ishiguro T et al. Sci Rep 2018 May 16;8(1):7671
PMID:40520116 - Histone deacetylation as a landmark for Sgo2 relocation from centromeres to subtelomeres during interphase.
Osaki M et al. iScience 2025 Jun 20;28(6):112717
PMID:11927555 - The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity.
Krien MJ et al. EMBO J 2002 Apr 02;21(7):1713-22
PMID:16541024 - Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.
Riedel CG et al. Nature 2006 May 04;441(7089):53-61
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.
Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:37288768 - Altered cohesin dynamics and H3K9 modifications contribute to mitotic defects in the cbf11Δ lipid metabolism mutant.
Vishwanatha A et al. J Cell Sci 2023 Jun 01;136(11)
PMID:9864354 - Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis.
Bernard P et al. J Cell Biol 1998 Dec 28;143(7):1775-87
PMID:11909965 - Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner.
Millband DN et al. Mol Cell Biol 2002 Apr;22(8):2728-42
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:38097187 - Phosphorylation of Bub1 by Mph1 promotes the Bub1 signaling at the kinetochore to ensure accurate chromosome segregation.
Jian Y et al. J Biol Chem 2023 Dec 12;:105559
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.
Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)
PMID:15509783 - Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p.
Vanoosthuyse V et al. Mol Cell Biol 2004 Nov;24(22):9786-801
PMID:22825872 - Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.
Heinrich S et al. J Cell Sci 2012 Oct 15;125(Pt 20):4720-7
PMID:15743824 - Inactivation of the pre-mRNA cleavage and polyadenylation factor Pfs2 in fission yeast causes lethal cell cycle defects.
Wang SW et al. Mol Cell Biol 2005 Mar;25(6):2288-96
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8
PMID:26518661 - The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.
Sadeghi L et al. EMBO Rep 2015 Dec;16(12):1673-87
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:27618268 - Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.
Mora-Santos MD et al. Curr Biol 2016 Oct 10;26(19):2642-2650
PMID:32406497 - Establishing correct kinetochore-microtubule attachments in mitosis and meiosis.
Cairo G et al. Essays Biochem 2020 Sep 04;64(2):277-287
PMID:24469396 - Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.
Nakano A et al. Mol Cell Biol 2014 Apr;34(8):1389-97
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.
Jian Y et al. J Cell Sci 2023 Jan 15;136(2)
PMID:26258632 - Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.
Akera T et al. Nat Cell Biol 2015 Sep;17(9):1124-33
PMID:15146064 - Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast.
Tournier S et al. Mol Biol Cell 2004 Jul;15(7):3345-56
PMID:15483052 - BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.
Minoda A et al. Mol Biol Cell 2005 Jan;16(1):316-27
PMID:38166399 - Fission yeast Wee1 is required for stable kinetochore-microtubule attachment.
Takado M et al. Open Biol 2024 Jan;14(1):230379
PMID:15347659 - DNA replication checkpoint control mediated by the spindle checkpoint protein Mad2p in fission yeast.
Sugimoto I et al. J Biol Chem 2004 Nov 05;279(45):47372-8
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:12620220 - The telomere protein Taz1 is required to prevent and repair genomic DNA breaks.
Miller KM et al. Mol Cell 2003 Feb;11(2):303-13
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:16478992 - Requirement of fission yeast Cid14 in polyadenylation of rRNAs.
Win TZ et al. Mol Cell Biol 2006 Mar;26(5):1710-21
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:22184248 - Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.
Ito D et al. Proc Natl Acad Sci U S A 2012 Jan 03;109(1):209-14
PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.
Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:12065422 - Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.
Grallert A et al. EMBO J 2002 Jun 17;21(12):3096-107
PMID:22375062 - Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.
Buttrick GJ et al. J Cell Sci 2012 Apr 01;125(Pt 7):1645-51
PMID:28567704 - Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity.
Sonkar A et al. Mol Genet Genomics 2017 Oct;292(5):1027-1036
PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.
Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47
PMID:17277378 - Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis.
Tange Y et al. Genetics 2007 Apr;175(4):1571-84
PMID:11331883 - Fission yeast Bub1 is essential in setting up the meiotic pattern of chromosome segregation.
Bernard P et al. Nat Cell Biol 2001 May;3(5):522-6
PMID:18632983 - Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction.
Saitoh S et al. Mol Biol Cell 2008 Sep;19(9):3885-97
PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.
Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9
PMID:11882285 - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.
Toyoda Y et al. Curr Biol 2002 Mar 05;12(5):347-58
PMID:16179942 - Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.
Asakawa K et al. EMBO Rep 2005 Dec;6(12):1194-200
PMID:11069763 - Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.
Pidoux AL et al. J Cell Sci 2000 Dec;113 Pt 23:4177-91
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.
Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:20929775 - Two histone marks establish the inner centromere and chromosome bi-orientation.
Yamagishi Y et al. Science 2010 Oct 08;330(6001):239-43
PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.
Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15525673 - The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function.
Kadura S et al. Mol Biol Cell 2005 Jan;16(1):385-95
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6