protein coding gene - bub1 (SPCC1322.12c) - mitotic spindle checkpoint kinase Bub1

Gene summary

Standard name

bub1

Systematic ID

SPCC1322.12c

Product

mitotic spindle checkpoint kinase Bub1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O94751

ORFeome ID

30/30A12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 1312560..1315997 reverse strand

Annotation

Protein features

Orthologs

• BUB1 (HGNC:1148) Homo sapiens
• BUB1B (HGNC:1149) Homo sapiens
• bub1 (SJAG_01082) Schizosaccharomyces japonicus
• BUB1 (YGR188C) Saccharomyces cerevisiae

References / Literature

PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.

Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7

PMID:26900649 - The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.

Chmielewska AE et al. Cell Cycle 2016;15(5):740-7

PMID:34738170 - Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe.

Fukunaga T et al. Appl Microbiol Biotechnol 2021 Dec;105(23):8771-8781

PMID:29769606 - Malonylation of histone H2A at lysine 119 inhibits Bub1-dependent H2A phosphorylation and chromosomal localization of shugoshin proteins.

Ishiguro T et al. Sci Rep 2018 May 16;8(1):7671

PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.

Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23

PMID:12426374 - Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast.

Garcia MA et al. EMBO J 2002 Nov 15;21(22):6015-24

PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.

Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8

PMID:22184248 - Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.

Ito D et al. Proc Natl Acad Sci U S A 2012 Jan 03;109(1):209-14

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

PMID:22375062 - Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.

Buttrick GJ et al. J Cell Sci 2012 Apr 01;125(Pt 7):1645-51

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.

Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39

PMID:11331883 - Fission yeast Bub1 is essential in setting up the meiotic pattern of chromosome segregation.

Bernard P et al. Nat Cell Biol 2001 May;3(5):522-6

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:15146064 - Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast.

Tournier S et al. Mol Biol Cell 2004 Jul;15(7):3345-56

PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:16179942 - Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.

Asakawa K et al. EMBO Rep 2005 Dec;6(12):1194-200

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.

Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:14730319 - The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis.

Kitajima TS et al. Nature 2004 Feb 05;427(6974):510-7

PMID:15347659 - DNA replication checkpoint control mediated by the spindle checkpoint protein Mad2p in fission yeast.

Sugimoto I et al. J Biol Chem 2004 Nov 05;279(45):47372-8

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:11861765 - Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest.

Wang SW et al. J Cell Sci 2002 Feb 01;115(Pt 3):587-98

PMID:28567704 - Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity.

Sonkar A et al. Mol Genet Genomics 2017 Oct;292(5):1027-1036

PMID:20929775 - Two histone marks establish the inner centromere and chromosome bi-orientation.

Yamagishi Y et al. Science 2010 Oct 08;330(6001):239-43

PMID:15743824 - Inactivation of the pre-mRNA cleavage and polyadenylation factor Pfs2 in fission yeast causes lethal cell cycle defects.

Wang SW et al. Mol Cell Biol 2005 Mar;25(6):2288-96

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:11927555 - The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity.

Krien MJ et al. EMBO J 2002 Apr 02;21(7):1713-22

PMID:12951601 - Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast.

Sato M et al. Nat Cell Biol 2003 Sep;5(9):764-6; author reply 766

PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.

Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36

PMID:12620220 - The telomere protein Taz1 is required to prevent and repair genomic DNA breaks.

Miller KM et al. Mol Cell 2003 Feb;11(2):303-13

PMID:11069763 - Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.

Pidoux AL et al. J Cell Sci 2000 Dec;113 Pt 23:4177-91

PMID:15509783 - Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p.

Vanoosthuyse V et al. Mol Cell Biol 2004 Nov;24(22):9786-801

PMID:15525673 - The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function.

Kadura S et al. Mol Biol Cell 2005 Jan;16(1):385-95

PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.

Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35

PMID:11882285 - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.

Toyoda Y et al. Curr Biol 2002 Mar 05;12(5):347-58

PMID:18062930 - Functional characterisation of the Schizosaccharomyces pombe homologue of the leukaemia-associated translocation breakpoint binding protein translin and its binding partner, TRAX.

Jaendling A et al. Biochim Biophys Acta 2008 Feb;1783(2):203-13

PMID:27618268 - Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.

Mora-Santos MD et al. Curr Biol 2016 Oct 10;26(19):2642-2650

PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.

Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:11909965 - Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner.

Millband DN et al. Mol Cell Biol 2002 Apr;22(8):2728-42

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:12065422 - Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.

Grallert A et al. EMBO J 2002 Jun 17;21(12):3096-107

PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.

Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77

PMID:15483052 - BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.

Minoda A et al. Mol Biol Cell 2005 Jan;16(1):316-27

PMID:17380189 - Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.

Murakami H et al. PLoS One 2007 Mar 21;2(3):e317

PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.

Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.

Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307

PMID:22281223 - Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.

Zich J et al. Curr Biol 2012 Feb 21;22(4):296-301

PMID:24469396 - Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.

Nakano A et al. Mol Cell Biol 2014 Apr;34(8):1389-97

PMID:26581324 - Cleavage and polyadenylation factor, Rna14 is an essential protein required for the maintenance of genomic integrity in fission yeast Schizosaccharomyces pombe.

Sonkar A et al. Biochim Biophys Acta 2016 Feb;1863(2):189-97

PMID:28017606 - Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.

Yuan I et al. Curr Biol 2017 Jan 09;27(1):137-143

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:16360688 - Control of Shugoshin function during fission-yeast meiosis.

Vaur S et al. Curr Biol 2005 Dec 20;15(24):2263-70

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:27798241 - An acetyltransferase-independent function of Eso1 regulates centromere cohesion.

Lin SJ et al. Mol Biol Cell 2016 Dec 15;27(25):4002-4010

PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.

Jian Y et al. J Cell Sci 2023 Jan 15;136(2)

PMID:26804021 - Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.

Tashiro S et al. Nat Commun 2016 Jan 25;7:10393

PMID:12606573 - Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint.

Yamaguchi S et al. EMBO J 2003 Mar 03;22(5):1075-87

PMID:38097187 - Phosphorylation of Bub1 by Mph1 promotes the Bub1 signaling at the kinetochore to ensure accurate chromosome segregation.

Jian Y et al. J Biol Chem 2023 Dec 12;:105559

PMID:38166399 - Fission yeast Wee1 is required for stable kinetochore-microtubule attachment.

Takado M et al. Open Biol 2024 Jan;14(1):230379

PMID:37288768 - Altered cohesin dynamics and H3K9 modifications contribute to mitotic defects in the cbf11Δ lipid metabolism mutant.

Vishwanatha A et al. J Cell Sci 2023 Jun 01;136(11)

PMID:9864354 - Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis.

Bernard P et al. J Cell Biol 1998 Dec 28;143(7):1775-87

PMID:19965387 - Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin.

Kawashima SA et al. Science 2010 Jan 08;327(5962):172-7

PMID:22660415 - MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.

Yamagishi Y et al. Nat Cell Biol 2012 Jun 03;14(7):746-52

PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.

Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9

PMID:32406497 - Establishing correct kinetochore-microtubule attachments in mitosis and meiosis.

Cairo G et al. Essays Biochem 2020 Sep 04;64(2):277-287

PMID:16478992 - Requirement of fission yeast Cid14 in polyadenylation of rRNAs.

Win TZ et al. Mol Cell Biol 2006 Mar;26(5):1710-21

PMID:16541024 - Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.

Riedel CG et al. Nature 2006 May 04;441(7089):53-61

PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.

Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659

PMID:19680287 - Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.

Windecker H et al. EMBO Rep 2009 Sep;10(9):1022-8

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:40520116 - Histone deacetylation as a landmark for Sgo2 relocation from centromeres to subtelomeres during interphase.

Osaki M et al. iScience 2025 Jun 20;28(6):112717

PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.

Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)

PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.

Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:22825872 - Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.

Heinrich S et al. J Cell Sci 2012 Oct 15;125(Pt 20):4720-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:11870212 - Kinesins klp5(+) and klp6(+) are required for normal chromosome movement in mitosis.

West RR et al. J Cell Sci 2002 Mar 01;115(Pt 5):931-40

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47

PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.

Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:17277378 - Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis.

Tange Y et al. Genetics 2007 Apr;175(4):1571-84

PMID:19523829 - Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation.

Choi SH et al. Curr Biol 2009 Jun 23;19(12):985-95

PMID:26518661 - The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.

Sadeghi L et al. EMBO Rep 2015 Dec;16(12):1673-87

PMID:18632983 - Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction.

Saitoh S et al. Mol Biol Cell 2008 Sep;19(9):3885-97

PMID:26258632 - Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.

Akera T et al. Nat Cell Biol 2015 Sep;17(9):1124-33

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608