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protein coding gene - ade6 (SPCC1322.13) - phosphoribosylaminoimidazole carboxylase Ade6

Gene summary

Standard name
ade6
Systematic ID
SPCC1322.13
Product
phosphoribosylaminoimidazole carboxylase Ade6
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
min1
UniProt ID
P15567
ORFeome ID
34/34G10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1316281..1318035 forward strand

Annotation

PBO:0005452 - 4.1.1.21

Disease association

MONDO:0859244 - phosphoribosylaminoimidazole carboxylase deficiency

References:

GO biological process

GO:0006189 - 'de novo' IMP biosynthetic process

References:

GO:0009113 - purine nucleobase biosynthetic process

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GO cellular component

GO:0005829 - cytosol

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GO molecular function

GO:0005524 - ATP binding

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GO:0046872 - metal ion binding

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GO:0004638 - phosphoribosylaminoimidazole carboxylase activity

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Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000016 - curved vegetative cell

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Genotypes:

FYPO:0000711 - decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0001570 - decreased cellular glutathione level during vegetative growth

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Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000747 - growth auxotrophic for adenine

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Genotypes:

FYPO:0003270 - growth auxotrophic for glutathione

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Genotypes:

FYPO:0004582 - increased cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

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Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000740 - normal cellular pigment accumulation

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0002865 - sensitive to polypeptone

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0000112 - sensitive to sorbitol

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Genotypes:

FYPO:0002067 - slow cell population growth during recovery from stationary phase

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0003911 - UGA suppression

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Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000747 - growth auxotrophic for adenine

References:

Genotypes:

FYPO:0002243 - increased acid phosphatase activity

References:

Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0004057 - increased intragenic meiotic recombination

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Genotypes:

FYPO:0001242 - increased RNA level during cellular response to adenine starvation

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Genotypes:

FYPO:0002728 - inviable swollen elongated cell with enlarged nucleus upon entry into stationary phase

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Genotypes:

FYPO:0002732 - mononucleate cell with increased DNA content during stationary phase

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Genotypes:

FYPO:0001988 - normal cell population growth at high pH

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Genotypes:

FYPO:0000740 - normal cellular pigment accumulation

References:

Genotypes:

FYPO:0003891 - normal intragenic meiotic recombination

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF22660RS_preATP-grasp-likePurT/PurK_preATP-graspPFAM
PF17769PurK_CPurK_CPFAM
PF00731AIRCPurE_domPFAM
PF02222ATP-graspATP-grasp_carboxylate-aminePFAM
PS50975ATP_GRASPATP-graspPROSITE_PROFILES
SM01001AIRC_2PurE_domSMART
G3DSA:3.40.50.20:FF:000030FUNFAM
G3DSA:3.40.50.1970:FF:000013FUNFAM
G3DSA:3.30.470.20:FF:000037FUNFAM
G3DSA:3.30.1490.20:FF:000016FUNFAM
SSF51246Rudiment single hybrid motifRudment_hybrid_motifSUPERFAMILY
SSF52440PreATP-grasp domainPreATP-grasp_dom_sfSUPERFAMILY
SSF56059Glutathione synthetase ATP-binding domain-likeSUPERFAMILY
SSF52255N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)SUPERFAMILY
G3DSA:3.40.50.20GENE3D
G3DSA:3.30.470.20GENE3D
G3DSA:3.30.1490.20ATP_grasp_subdomain_1GENE3D
G3DSA:3.40.50.1970GENE3D
PTHR11609PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7PANTHER
PIRSF001340AIRCAde2_fungi/plantPIRSF
MF_01928PurKPurKHAMAP
MF_01929PurE_classIPurE_ClassIHAMAP
TIGR01162purEPurE_domNCBIFAM
NF004679PRK06019.1-5NCBIFAM
TIGR01161purKPurKNCBIFAM

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:31712578 - DNA sequence differences are determinants of meiotic recombination outcome.
Brown SD et al. Sci Rep 2019 Nov 11;9(1):16446
PMID:16115814 - Pas1, a G1 cyclin, regulates amino acid uptake and rescues a delay in G1 arrest in Tsc1 and Tsc2 mutants in Schizosaccharomyces pombe.
van Slegtenhorst M et al. Hum Mol Genet 2005 Oct 01;14(19):2851-8
PMID:22992726 - The SAGA histone acetyltransferase complex regulates leucine uptake through the Agp3 permease in fission yeast.
Takahashi H et al. J Biol Chem 2012 Nov 02;287(45):38158-67
PMID:29632066 - TOR complex 2 in fission yeast is required for chromatin-mediated gene silencing and assembly of heterochromatic domains at subtelomeres.
Cohen A et al. J Biol Chem 2018 May 25;293(21):8138-8150
PMID:2806887 - Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
Ponticelli AS et al. Genetics 1989 Sep;123(1):45-54
PMID:1394510 - Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.
Schweingruber ME et al. Curr Genet 1992 Oct;22(4):289-92
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25519804 - Nonsense codon suppression in fission yeast due to mutations of tRNA(Ser.11) and translation release factor Sup35 (eRF3).
Protacio RU et al. Curr Genet 2015 May;61(2):165-73
PMID:19436749 - Phosphorylation-independent regulation of Atf1-promoted meiotic recombination by stress-activated, p38 kinase Spc1 of fission yeast.
Gao J et al. PLoS One 2009;4(5):e5533
PMID:8005432 - Transient, meiosis-induced expression of the rec6 and rec12 genes of Schizosaccharomyces pombe.
Lin Y et al. Genetics 1994 Mar;136(3):769-79
GO_REF:0000002 - Comments
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:9017391 - apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump.
Chaudhuri B et al. Genetics 1997 Jan;145(1):75-83
PMID:3221399 - DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination host spot allele ade6-M26.
Szankasi P et al. J Mol Biol 1988 Dec 20;204(4):917-25
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:12136010 - Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe.
Matsumoto S et al. Genetics 2002 Jul;161(3):1053-63
PMID:9076725 - Region-specific meiotic recombination in Schizosaccharomyces pombe: the rec11 gene.
Li YF et al. Mol Microbiol 1997 Mar;23(5):869-78
PMID:9671035 - Global control of meiotic recombination genes by Schizosaccharomyces pombe rec16 (rep1).
Ding R et al. Mol Gen Genet 1998 Jun;258(6):663-70
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:14718525 - Tsc1+ and tsc2+ regulate arginine uptake and metabolism in Schizosaccharomyces pombe.
van Slegtenhorst M et al. J Biol Chem 2004 Mar 26;279(13):12706-13
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:7262540 - Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe.
Nurse P et al. Genetics 1980 Nov;96(3):627-37
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29784772 - A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.
Wang J et al. Mol Cell Biol 2018 Aug 01;38(15)
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:25352017 - Implementation of the CRISPR-Cas9 system in fission yeast.
Jacobs JZ et al. Nat Commun 2014 Oct 29;5:5344
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:1448066 - Purine biosynthetic genes are required for cadmium tolerance in Schizosaccharomyces pombe.
Speiser DM et al. Mol Cell Biol 1992 Dec;12(12):5301-10
PMID:32295063 - The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe .
Marayati BF et al. Cells 2020 Apr 13;9(4)
PMID:39527204 - Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.
Protacio RU et al. Methods Mol Biol 2025;2862:223-239
PMID:499806 - Allelism of methionine-sensitive mutants of Schizosaccharomyces pombe to loci involved in adenine biosynthesis.
Strauss A Genet Res 1979 Jun;33(3):261-8
PMID:8299939 - Region-specific activators of meiotic recombination in Schizosaccharomyces pombe.
DeVeaux LC et al. Genes Dev 1994 Jan;8(2):203-10
PMID:7565608 - Schizosaccharomyces pombe rds1, an adenine-repressible gene regulated by glucose, ammonium, phosphate, carbon dioxide and temperature.
Ludin KM et al. Mol Gen Genet 1995 Aug 30;248(4):439-45
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:8625425 - pH sensitivity of Schizosaccharomyces pombe: effect on the cellular phenotype associated with lacZ gene expression.
Arndt GM et al. Curr Genet 1996 Apr;29(5):457-61
PMID:23145069 - Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe.
Krzyzanowski MK et al. PLoS One 2012;7(11):e49059
PMID:7855597 - A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction.
Szankasi P et al. Science 1995 Feb 24;267(5201):1166-9
PMID:27613427 - Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.
Maksimov V et al. Curr Genet 2017 May;63(2):343-357
PMID:30640914 - CDK contribution to DSB formation and recombination in fission yeast meiosis.
Bustamante-Jaramillo LF et al. PLoS Genet 2019 Jan;15(1):e1007876
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:2743431 - An abnormal cell division cycle in an AIR carboxylase-deficient mutant of the fission yeast Schizosaccharomyces pombe.
Ishiguro J Curr Genet 1989 Jan;15(1):71-4
PMID:17261596 - Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast.
Matsuo T et al. Mol Cell Biol 2007 Apr;27(8):3154-64
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:15292231 - Regulation of Swi6/HP1-dependent heterochromatin assembly by cooperation of components of the mitogen-activated protein kinase pathway and a histone deacetylase Clr6.
Kim HS et al. J Biol Chem 2004 Oct 08;279(41):42850-9
PMID:18257391 - Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processing.
Park JM et al. Gene Expr 2007;14(2):71-81
PMID:28469148 - Functional organization of protein determinants of meiotic DNA break hotspots.
Ma L et al. Sci Rep 2017 May 03;7(1):1393
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025