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protein coding gene - ctr4 (SPCC1393.10) - plasma membrane copper transporter complex subunit Ctr4

Gene summary

Standard name
ctr4
Systematic ID
SPCC1393.10
Product
plasma membrane copper transporter complex subunit Ctr4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94722
ORFeome ID
11/11G05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 818459..819361 forward strand

Annotation

Comment

PBO:0005291 - promoter contains CuSE

Disease association

MONDO:0957211 - neurodegeneration and seizures due to copper transport defect

References:

GO biological process

GO:0015677 - copper ion import

References:

GO:0098705 - copper ion import across plasma membrane

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GO cellular component

GO:0005886 - plasma membrane

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GO molecular function

GO:0005375 - copper ion transmembrane transporter activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0003805 - decreased cell population growth on non-fermentable carbon source

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Genotypes:

FYPO:0003284 - decreased copper import

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Genotypes:

FYPO:0003073 - decreased primary amine oxidase activity

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Genotypes:

FYPO:0003282 - decreased superoxide dismutase activity

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0004931 - normal RNA level during sporulation

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Genotypes:

FYPO:0000726 - sensitive to oxidative stress

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Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000015 - RNA absent

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0001934 - abolished cell population growth on glycerol carbon source

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Genotypes:

FYPO:0003690 - abolished cell population growth on glycerol/ethanol carbon source

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Genotypes:

FYPO:0004039 - abolished primary amine oxidase activity

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Genotypes:

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0003284 - decreased copper import

References:

Genotypes:

FYPO:0003073 - decreased primary amine oxidase activity

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Genotypes:

FYPO:0003282 - decreased superoxide dismutase activity

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Genotypes:

FYPO:0004180 - increased cellular protein aggregate level

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0006212 - inviable after spore germination, before germ tube formation, during copper ion starvation

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001409 - normal growth on glycerol carbon source

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Genotypes:

FYPO:0004931 - normal RNA level during sporulation

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Genotypes:

FYPO:0005977 - prion formation

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0004669 - sensitive to ferrozine

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0001987 - sensitive to high pH

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0002015 - sensitive to iron ion starvation

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000726 - sensitive to oxidative stress

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015221 - heteromeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF04145CtrCop_transporterPfam
PTHR12483Cop_transporterPANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:28572514 - Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.
Plante S et al. J Biol Chem 2017 Jul 14;292(28):11896-11914
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28882432 - Analysis of ambient pH stress response mediated by iron and copper intake in Schizosaccharomyces pombe.
Higuchi Y et al. J Biosci Bioeng 2018 Jan;125(1):92-96
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:28191457 - The copper transport-associated protein Ctr4 can form prion-like epigenetic determinants in Schizosaccharomyces pombe .
Sideri T et al. Microb Cell 2017 Jan;4(1):16-28
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:10593913 - A copper-sensing transcription factor regulates iron uptake genes in Schizosaccharomyces pombe.
Labbé S et al. J Biol Chem 1999 Dec 17;274(51):36252-60
PMID:12578838 - The Schizosaccharomyces pombe Cuf1 is composed of functional modules from two distinct classes of copper metalloregulatory transcription factors.
Beaudoin J et al. J Biol Chem 2003 Apr 18;278(16):14565-77
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:23122962 - Cdk11-cyclinL controls the assembly of the RNA polymerase II mediator complex.
Drogat J et al. Cell Rep 2012 Nov 29;2(5):1068-76
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:12244050 - Ctr6, a vacuolar membrane copper transporter in Schizosaccharomyces pombe.
Bellemare DR et al. J Biol Chem 2002 Nov 29;277(48):46676-86
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.
Yu Y et al. Sci Rep 2016 Aug 19;6:31752
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20694150 - Copper-dependent trafficking of the Ctr4-Ctr5 copper transporting complex.
Ioannoni R et al. PLoS One 2010 Aug 04;5(8):e11964
PMID:24569997 - Characterization of Schizosaccharomyces pombe copper transporter proteins in meiotic and sporulating cells.
Plante S et al. J Biol Chem 2014 Apr 04;289(14):10168-81
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:17477863 - Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study.
Rustici G et al. Genome Biol 2007;8(5):R73
PMID:18723604 - Copper distributed by Atx1 is available to copper amine oxidase 1 in Schizosaccharomyces pombe.
Peter C et al. Eukaryot Cell 2008 Oct;7(10):1781-94
PMID:11274192 - Identification of a novel high affinity copper transport complex in the fission yeast Schizosaccharomyces pombe.
Zhou H et al. J Biol Chem 2001 Jun 08;276(23):20529-35
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:28357387 - S. pombe placed on the prion map.
Hayles J Microb Cell 2017 Feb 03;4(2):35-37
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:16385131 - Functional dissection of Ctr4 and Ctr5 amino-terminal regions reveals motifs with redundant roles in copper transport.
Beaudoin J et al. Microbiology (Reading) 2006 Jan;152(Pt 1):209-222
PMID:17384198 - Crm1-mediated nuclear export of the Schizosaccharomyces pombe transcription factor Cuf1 during a shift from low to high copper concentrations.
Beaudoin J et al. Eukaryot Cell 2007 May;6(5):764-75
PMID:21828039 - Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Beaudoin J et al. J Biol Chem 2011 Sep 30;286(39):34356-72
PMID:22558440 - Cuf2 is a novel meiosis-specific regulatory factor of meiosis maturation.
Ioannoni R et al. PLoS One 2012;7(4):e36338
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:24167631 - Klf1, a C2H2 zinc finger-transcription factor, is required for cell wall maintenance during long-term quiescence in differentiated G0 phase.
Shimanuki M et al. PLoS One 2013;8(10):e78545
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9