protein coding gene - rpb3 (SPCC1442.10c) - DNA-directed RNA polymerase II complex subunit Rpb3

Gene summary

Standard name

rpb3

Systematic ID

SPCC1442.10c

Product

DNA-directed RNA polymerase II complex subunit Rpb3

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P37382

ORFeome ID

14/14B12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 1782897..1786998 reverse strand

Annotation

Protein features

Orthologs

• POLR2C (HGNC:9189) Homo sapiens
• rpb3 (SJAG_02506) Schizosaccharomyces japonicus
• RPB3 (YIL021W) Saccharomyces cerevisiae

References / Literature

PMID:9642054 - Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.

Ishiguro A et al. J Mol Biol 1998 Jun 19;279(4):703-12

PMID:10503538 - Isolation and characterization of temperature-sensitive mutations in the gene (rpb3) for subunit 3 of RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Mitobe J et al. Mol Gen Genet 1999 Aug;262(1):73-84

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:8367291 - Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene.

Azuma Y et al. Nucleic Acids Res 1993 Aug 11;21(16):3749-54

PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.

Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9

PMID:11453250 - Functional analysis of RNA polymerase II Rpb3 mutants of the fission yeast Schizosaccharomyces pombe.

Mitobe J et al. Curr Genet 2001 Jun;39(4):210-21

PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88

PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.

Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:28652406 - Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation.

Salat-Canela C et al. J Biol Chem 2017 Aug 18;292(33):13635-13644

PMID:24583182 - A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Suzuki S et al. Protein Expr Purif 2014 May;97:44-9

PMID:39746995 - DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.

Kuś K et al. Nat Commun 2025 Jan 02;16(1):10

PMID:9322754 - Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.

Sakurai H et al. Gene 1997 Sep 01;196(1-2):165-74

PMID:11918671 - Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.

Sakurai H et al. Genes Cells 2002 Mar;7(3):273-84

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:9077438 - Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5.

Miyao T et al. Genes Cells 1996 Sep;1(9):843-54

PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.

Okita AK et al. Commun Biol 2019;2:17

PMID:10523639 - The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.

Sakurai H et al. Mol Cell Biol 1999 Nov;19(11):7511-8

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:19682301 - A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast.

Paul SK et al. FEBS J 2009 Sep;276(18):5076-93

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6

PMID:26792892 - Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Suzuki S et al. Nucleic Acids Res 2016 May 19;44(9):4147-62

PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:37956308 - Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery.

Vega M et al. Nucleic Acids Res 2023 Dec 11;51(22):12161-12173

PMID:9548938 - Location of subunit-subunit contact sites on RNA polymerase II subunit 3 from the fission yeast Schizosaccharomyces pombe.

Yasui K et al. Biochemistry 1998 Apr 21;37(16):5542-8

PMID:9738888 - Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast.

Miyao T et al. Mol Gen Genet 1998 Jul;259(1):123-9

PMID:10625684 - Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.

Spåhr H et al. J Biol Chem 2000 Jan 14;275(2):1351-6

PMID:12552808 - [Mapping the interaction site of Rpb2 and Rpb3 subunit of fission yeast RNA polymerase II].

Qu Z et al. Wei Sheng Wu Xue Bao 2001 Oct;41(5):592-7

PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.

Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055

PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.

Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72

PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.

Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201

PMID:30282034 - Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination.

Kecman T et al. Cell Rep 2018 Oct 02;25(1):259-269.e5

PMID:9325316 - RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.

Kimura M et al. J Biol Chem 1997 Oct 10;272(41):25851-5

PMID:32059768 - Size-Dependent Increase in RNA Polymerase II Initiation Rates Mediates Gene Expression Scaling with Cell Size.

Sun XM et al. Curr Biol 2020 Apr 06;30(7):1217-1230.e7

PMID:28366642 - Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.

Shetty A et al. Mol Cell 2017 Apr 06;66(1):77-88.e5

PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:20605454 - A gene-specific requirement of RNA polymerase II CTD phosphorylation for sexual differentiation in S. pombe.

Coudreuse D et al. Curr Biol 2010 Jun 22;20(12):1053-64