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protein coding gene - trm732 (SPCC1494.07) - tRNA (cytosine 32-2'-O)-methyltransferase regulator Trm732

Gene summary

Standard name
trm732
Systematic ID
SPCC1494.07
Product
tRNA (cytosine 32-2'-O)-methyltransferase regulator Trm732
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
trm72
UniProt ID
O60081
ORFeome ID
30/30H08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 2336555..2341383 forward strand

Annotation

Disease association

MONDO:0005032 - follicular thyroid adenoma

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GO biological process

GO:0030488 - tRNA methylation

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GO:0002128 - tRNA nucleoside ribose methylation

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0030234 - enzyme regulator activity

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Modification

MOD:00595 - monomannosylated residue

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MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0007359 - resistance to heavy water

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Genotypes:

Protein features

PBO:0111765 - HEAT repeat

Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0007021 - abolished tRNA cytosine 2'-O-methylation

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0007026 - decreased tRNA wybutosine biosynthesis

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0007023 - increased tRNA guanosine 1-methylation

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000840 - normal RNA level

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Genotypes:

FYPO:0007018 - normal tRNA guanosine 2'-O-methylation

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Genotypes:

FYPO:0007019 - normal tRNA guanosine 7-methylation

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Genotypes:

FYPO:0007020 - normal tRNA guanosine N2-methylation

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Genotypes:

FYPO:0007017 - normal tRNA pseudouridylation

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009035 - resistance to formamide

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10350DUF2428THADA/TRM732_DUF2428PFAM
PF25151TPR_Trm732_CTHADA-like_TPR_CPFAM
PF25150TPR_Trm732THADA-like_TPRPFAM
PF26523Trm732_CTrm732_CPFAM
SSF48371ARM repeatARM-type_foldSUPERFAMILY
PTHR14387THADA/DEATH RECEPTOR INTERACTING PROTEINtRNA_methyltransferase_THADAPANTHER

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PB_REF:0000003 - Disease Association Curation
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25404562 - Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes.
Guy MP et al. RNA 2015 Jan;21(1):61-74
PMID:29596413 - Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.
Han L et al. PLoS Genet 2018 Mar;14(3):e1007288
PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.
Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399