PomBase - protein coding gene - sap1 (SPCC1672.02c) - switch-activating protein Sap1 - The Schizosaccharomyces pombe genome database

ID	Name	InterPro name	DB name
PF21438	Sap1_N	Sap1_N	Pfam
Coil			COILS
mobidb-lite-Disorder	disorder_prediction		MobiDB-Disorder
mobidb-lite-Low-complexity	disorder_prediction		MobiDB-Low-complexity
mobidb-lite-Polyampholyte	disorder_prediction		MobiDB-Polyampholyte

References / Literature

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.: Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:29610759 - Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.: Raimondi C et al. Microb Cell 2018 Jan 16;5(4):169-183
PMID:16195226 - Sap1p binds to Ter1 at the ribosomal DNA of Schizosaccharomyces pombe and causes polar replication fork arrest.: Krings G et al. J Biol Chem 2005 Nov 25;280(47):39135-42
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.: Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.: Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.: Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.: Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21151105 - CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR.: Zaratiegui M et al. Nature 2011 Jan 06;469(7328):112-5
PMID:7651412 - The essential DNA-binding protein sap1 of Schizosaccharomyces pombe contains two independent oligomerization interfaces that dictate the relative orientation of the DNA-binding domain.: Ghazvini M et al. Mol Cell Biol 1995 Sep;15(9):4939-46
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.: Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90
PMID:10884352 - Solution structural studies and low-resolution model of the Schizosaccharomyces pombe sap1 protein.: Bada M et al. J Mol Biol 2000 Jul 14;300(3):563-74
PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:17151242 - Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe.: Noguchi C et al. Genetics 2007 Feb;175(2):553-66
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.: Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:28223353 - Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe .: Guan L et al. J Biol Chem 2017 Apr 14;292(15):6056-6075
PMID:28490498 - Shelterin components mediate genome reorganization in response to replication stress.: Mizuguchi T et al. Proc Natl Acad Sci U S A 2017 May 23;114(21):5479-5484
PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.: Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:8065904 - Identification of the DNA-binding domains of the switch-activating-protein Sap1 from S.pombe by random point mutations screening in E.coli.: Arcangioli B et al. Nucleic Acids Res 1994 Aug 11;22(15):2930-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.: Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.: Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:18257517 - Phosphoproteome analysis of fission yeast.: Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:1915277 - A novel switch-activating site (SAS1) and its cognate binding factor (SAP1) required for efficient mat1 switching in Schizosaccharomyces pombe.: Arcangioli B et al. EMBO J 1991 Oct;10(10):3025-32
PMID:30026545 - Structure of the replication regulator Sap1 reveals functionally important interfaces.: Jørgensen MM et al. Sci Rep 2018 Jul 19;8(1):10930
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:8114737 - Sap1, a protein that binds to sequences required for mating-type switching, is essential for viability in Schizosaccharomyces pombe.: Arcangioli B et al. Mol Cell Biol 1994 Mar;14(3):2058-65
PMID:26358720 - Single-Nucleotide-Specific Targeting of the Tf1 Retrotransposon Promoted by the DNA-Binding Protein Sap1 of Schizosaccharomyces pombe.: Hickey A et al. Genetics 2015 Nov;201(3):905-24
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.: Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .: Budden AM et al. Elife 2023 Aug 24;12
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.: Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:23779158 - The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.: Saito Y et al. Sci Rep 2013;3:2022
PMID:16166653 - The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.: Mejía-Ramírez E et al. Mol Cell Biol 2005 Oct;25(19):8755-61
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

protein coding gene - sap1 (SPCC1672.02c) - switch-activating protein Sap1

Gene summary

Annotation

Experimental tools

PBO:0002742 - structure

GO biological process

GO:0007534 - gene conversion at mating-type locus

GO:1902985 - mitotic pre-replicative complex assembly

GO:0031582 - replication fork arrest at rDNA repeats

GO:0071170 - site-specific DNA replication termination

GO cellular component

GO:0000785 - chromatin

GO:0005634 - nucleus

GO molecular function

GO:0003677 - DNA binding

GO:0008301 - DNA binding, bending

GO:0005515 - protein binding

GO:0043110 - rDNA spacer replication fork barrier binding

GO:0043565 - sequence-specific DNA binding

GO:1990837 - sequence-specific double-stranded DNA binding

Modification

MOD:00046 - O-phospho-L-serine

MOD:00047 - O-phospho-L-threonine

MOD:00696 - phosphorylated residue

MOD:01149 - sumoylated lysine

MOD:01148 - ubiquitinylated lysine

Multi-locus phenotype

FYPO:0001357 - normal vegetative cell population growth

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

Quantitative gene expression

PBO:0006310 - protein level

PBO:0011963 - RNA level

Single locus phenotype

FYPO:0000659 - abolished DNA binding

FYPO:0000705 - abolished protein-protein interaction

FYPO:0003735 - altered DNA binding specificity

FYPO:0000658 - decreased DNA binding

FYPO:0000657 - increased DNA binding

FYPO:0002151 - inviable spore

FYPO:0002061 - inviable vegetative cell population

FYPO:0000655 - normal DNA binding

FYPO:0002489 - spore lysis

Subunit composition

PBO:0015212 - homomeric(2)

Taxonomic conservation

PBO:0016885 - Schizosaccharomyces specific

Protein features

Orthologs

References / Literature