protein coding gene - mis16 (SPCC1672.10) - histone H4 chaperone, CENP-A recruiting complex CENP-A chaperone, WD repeat subunit Mis16

Gene summary

Standard name

mis16

Systematic ID

SPCC1672.10

Product

histone H4 chaperone, CENP-A recruiting complex CENP-A chaperone, WD repeat subunit Mis16

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O94244

ORFeome ID

23/23D08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 584126..585893 forward strand

Annotation

Protein features

Orthologs

• RBBP4 (HGNC:9887) Homo sapiens
• RBBP7 (HGNC:9890) Homo sapiens
• mis16 (SJAG_03867) Schizosaccharomyces japonicus
• HAT2 (YEL056W) Saccharomyces cerevisiae

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .

Gao C et al. Genetics 2017 Oct;207(2):559-569

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:18362178 - Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.

Nakazawa N et al. J Cell Biol 2008 Mar 24;180(6):1115-31

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:18493607 - A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.

Natsume T et al. PLoS One 2008 May 21;3(5):e2221

PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.

Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.

Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98

PMID:23091701 - Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.

Nakamura T et al. Open Biol 2012 Sep;2(9):120117

PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.

Hirai H et al. PLoS One 2014;9(11):e111905

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:26343758 - Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.

An S et al. J Mol Biol 2015 Oct 09;427(20):3230-3240

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:22771823 - Schizosaccharomyces pombe Hat1 (Kat1) is associated with Mis16 and is required for telomeric silencing.

Tong K et al. Eukaryot Cell 2012 Sep;11(9):1095-103

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:38084929 - The cysteine-rich domain in CENP-A chaperone Scm3HJURP ensures centromere targeting and kinetochore integrity.

Folco HD et al. Nucleic Acids Res 2023 Dec 12;

PMID:31371524 - Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.

Korntner-Vetter M et al. Life Sci Alliance 2019 Aug;2(4)

PMID:34810257 - Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.

Dong Q et al. Proc Natl Acad Sci U S A 2021 Nov 30;118(48)

PMID:19217404 - Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.

Pidoux AL et al. Mol Cell 2009 Feb 13;33(3):299-311

PMID:24789708 - Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.

Subramanian L et al. Open Biol 2014 Apr 30;4(4):140043

PMID:11751918 - The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein.

Taricani L et al. J Biol Chem 2002 Mar 22;277(12):10562-72

PMID:27666591 - Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading.

Dong Q et al. Mol Cell 2016 Oct 06;64(1):79-91

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:24774534 - Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex.

Hayashi T et al. Genes Cells 2014 Jul;19(7):541-54

PMID:29804820 - Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A Cnp1 -Specific Assembly Factor through Eic1 Interaction.

An S et al. Structure 2018 Jul 03;26(7):960-971.e4

PMID:19366728 - Genetic control of cellular quiescence in S. pombe.

Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.

Hayashi T et al. Cell 2004 Sep 17;118(6):715-29