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protein coding gene - mcm3 (SPCC1682.02c) - MCM complex subunit Mcm3

Gene summary

Standard name
mcm3
Systematic ID
SPCC1682.02c
Product
MCM complex subunit Mcm3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P30666
ORFeome ID
38/38G03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 371443..375324 reverse strand

Annotation

GO biological process

GO:0006271 - DNA strand elongation involved in DNA replication

References:

GO:0000727 - double-strand break repair via break-induced replication

References:

GO:1902975 - mitotic DNA replication initiation

References:

GO:0006279 - premeiotic DNA replication

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0042555 - MCM complex

References:

GO:0005656 - nuclear pre-replicative complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0003688 - DNA replication origin binding

References:

GO:0003697 - single-stranded DNA binding

References:

GO:0017116 - single-stranded DNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000022 - protein absent

References:

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000021 - protein present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000950 - elongated aseptate vegetative cell

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0001385 - inviable after spore germination, without cell division, cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00493MCMMCM_domPfam
PF14551MCM_NMCM_NPfam
PF17207MCM_OBMCM_OBPfam
PF17855MCM_lidMCM_lidPfam
PF23191WHD_MCM3_CWH_MCM3_CPfam
cd17754MCM3CDD
PS00847MCM_1MCM_CSPROSITE patterns
PS50051MCM_CTDMCM_domPROSITE profiles
SM00350MCMMCMSMART
SM00382AAAAAA+_ATPaseSMART
PR01657MCMFAMILYMCM_domPRINTS
PR01659MCMPROTEIN3Mcm3PRINTS
G3DSA:2.20.28.10:FF:000008CATH-FunFam
G3DSA:3.40.50.300:FF:000234CATH-FunFam
G3DSA:2.20.28.10CATH-Gene3D
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
G3DSA:3.30.1640.10CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR11630MCMPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:9705504 - Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p).
Sherman DA et al. Nucleic Acids Res 1998 Sep 01;26(17):3955-60
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:10588642 - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.
Pasion SG et al. Mol Biol Cell 1999 Dec;10(12):4043-57
PMID:21813639 - Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.
Ding L et al. J Biol Chem 2011 Sep 23;286(38):32918-30
PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.
Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80
PMID:16899242 - Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.
Namdar M et al. Exp Cell Res 2006 Oct 15;312(17):3360-9
PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.
Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8
GO_REF:0000002 - Comments
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:10770926 - Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.
Lee JK et al. J Biol Chem 2000 Jun 23;275(25):18871-8
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.
Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.
Liang DT et al. Genetics 2001 Oct;159(2):471-86
PMID:11973289 - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.
Gómez EB et al. Genetics 2002 Apr;160(4):1305-18
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.
Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9
PMID:17016471 - Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF.
Tafforeau L et al. EMBO J 2006 Oct 04;25(19):4547-56
PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.
Li JJ et al. FEBS Lett 2011 Dec 15;585(24):3850-5
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39094566 - Mrc1 regulates parental histone segregation and heterochromatin inheritance.
Toda T et al. Mol Cell 2024 Jul 23;
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15338237 - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.
Dolan WP et al. Chromosoma 2004 Sep;113(3):145-56
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:9366552 - A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast.
Adachi Y et al. Genes Cells 1997 Jul;2(7):467-79