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protein coding gene - rpn8 (SPCC1682.10) - 19S proteasome regulatory subunit Rpn8

Gene summary

Standard name
rpn8
Systematic ID
SPCC1682.10
Product
19S proteasome regulatory subunit Rpn8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74440
ORFeome ID
15/15B11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 390694..392092 forward strand

Annotation

GO biological process

GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005635 - nuclear envelope

References:

GO:0005634 - nucleus

References:

GO:0008541 - proteasome regulatory particle, lid subcomplex

References:

GO molecular function

GO:0008237 - metallopeptidase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF13012MitMem_regEIF3F/CSN6-like_CPFAM
PF01398JABJAMM/MPN+_domPFAM
cd08062MPN_RPN7_8MPN_RPN7_8CDD
PS50249MPNMPNPROSITE_PROFILES
SM00232pad1_6JAMM/MPN+_domSMART
G3DSA:3.40.140.10:FF:000004FUNFAM
G3DSA:3.40.140.10Cytidine Deaminase, domain 2GENE3D
PTHR10540EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATEDPANTHER

Orthologs

References / Literature

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16149916 - Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis.
Jossé L et al. Biochem J 2006 Jan 01;393(Pt 1):303-9
GO_REF:0000002 - Comments
PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.
Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)
PMID:24938783 - Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.
Leitner A et al. Proc Natl Acad Sci U S A 2014 Jul 01;111(26):9455-60
PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.
Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:39473973 - Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity.
Ruiz-Romero G et al. iScience 2024 Nov 15;27(11):111095
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.
Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5
PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.
Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.
Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121