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protein coding gene - rpt4 (SPCC1682.16) - 19S proteasome base subcomplex ATPase subunit Rpt4

Gene summary

Standard name
rpt4
Systematic ID
SPCC1682.16
Product
19S proteasome base subcomplex ATPase subunit Rpt4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPCC306.01
UniProt ID
O74445
ORFeome ID
06/06C02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 405601..407595 forward strand

Annotation

GO biological process

GO:0036503 - ERAD pathway

References:

GO:0045899 - positive regulation of RNA polymerase II transcription preinitiation complex assembly

References:

GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0008540 - proteasome regulatory particle, base subcomplex

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0036402 - proteasome-activating activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004542 - increased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0006369 - increased histone H3-K9 dimethylation at heterochromatin domain during vegetative growth

References:

Genotypes:

FYPO:0006373 - increased histone H3-K9 dimethylation at heterochromatin island during vegetative growth

References:

Genotypes:

FYPO:0006361 - increased histone H3-K9 dimethylation at subtelomeric heterochromatin during vegetative growth

References:

Genotypes:

FYPO:0006368 - normal spatial extent of centromeric heterochromatin assembly

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111792 - AAA family ATPase

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0004137 - decreased histone H3-K9 dimethylation at subtelomeric heterochromatin during vegetative growth

References:

Genotypes:

FYPO:0003230 - decreased histone H3-K9 methylation at heterochromatin island during vegetative growth

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0005918 - decreased protein localization to subtelomeric heterochromatin

References:

Genotypes:

FYPO:0005286 - heterochromatin assembly beyond boundary element IRC1L

References:

Genotypes:

FYPO:0004542 - increased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0006369 - increased histone H3-K9 dimethylation at heterochromatin domain during vegetative growth

References:

Genotypes:

FYPO:0000873 - increased histone H3-K9 dimethylation during vegetative growth

References:

Genotypes:

FYPO:0006109 - increased subtelomeric transcript-derived siRNA level

References:

Genotypes:

FYPO:0001023 - normal growth on cisplatin

References:

Genotypes:

FYPO:0005080 - normal protein localization to heterochromatin at centromere inner repeat

References:

Genotypes:

FYPO:0002389 - normal protein localization to heterochromatin at centromere outer repeat

References:

Genotypes:

FYPO:0003576 - normal protein localization to subtelomeric heterochromatin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF16450Prot_ATP_ID_OB_CProt_ATP_ID_OB_2ndPFAM
PF17862AAA_lid_3AAA_lid_3PFAM
PF00004AAAATPase_AAA_corePFAM
PS00674AAAATPase_AAA_CSPROSITE_PATTERNS
SM00382AAA_5AAA+_ATPaseSMART
G3DSA:2.40.50.140:FF:000027FUNFAM
G3DSA:3.40.50.300:FF:000034FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:1.10.8.60GENE3D
G3DSA:2.40.50.140NA-bd_OB-foldGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR2307326S PROTEASOME REGULATORY SUBUNIT26S_Proteasome_ATPasePANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25002536 - A novel histone deacetylase complex in the control of transcription and genome stability.
Zilio N et al. Mol Cell Biol 2014 Sep 15;34(18):3500-14
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.
Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
GO_REF:0000051 - S. pombe keyword mapping
PMID:22307589 - Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach.
Lasker K et al. Proc Natl Acad Sci U S A 2012 Jan 31;109(5):1380-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.
Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155
PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.
Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)
PMID:20976105 - Silencing mediated by the Schizosaccharomyces pombe HIRA complex is dependent upon the Hpc2-like protein, Hip4.
Anderson HE et al. PLoS One 2010 Oct 18;5(10):e13488
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:29214404 - The 19S proteasome regulates subtelomere silencing and facultative heterochromatin formation in fission yeast.
Seo HD et al. Curr Genet 2018 Jun;64(3):741-752
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.
Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.
Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:24938783 - Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.
Leitner A et al. Proc Natl Acad Sci U S A 2014 Jul 01;111(26):9455-60
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
GO_REF:0000002 - Comments