protein coding gene - mcm4 (SPCC16A11.17) - MCM complex subunit Mcm4/Cdc21

Gene summary

Standard name

mcm4

Systematic ID

SPCC16A11.17

Product

MCM complex subunit Mcm4/Cdc21

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

rid2, cdc21, SPCC24B10.01

UniProt ID

P29458

ORFeome ID

47/47B07

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 900388..903425 forward strand

Annotation

Protein features

Orthologs

• MCM4 (HGNC:6947) Homo sapiens
• mcm4 (SJAG_04210) Schizosaccharomyces japonicus
• MCM4 (YPR019W) Saccharomyces cerevisiae

References / Literature

PMID:32341083 - Active Replication Checkpoint Drives Genome Instability in Fission Yeast mcm4 Mutant.

Kim SM et al. Mol Cell Biol 2020 Jun 29;40(14)

PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.

Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58

PMID:1934126 - Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.

Grallert B et al. Curr Genet 1991 Aug;20(3):199-204

PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213

PMID:8313892 - The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb.

Ohi R et al. EMBO J 1994 Jan 15;13(2):471-83

PMID:23444842 - Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.

Xu M et al. BMC Biochem 2013 Feb 27;14:5

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:12975364 - Substrate requirements for duplex DNA translocation by the eukaryal and archaeal minichromosome maintenance helicases.

Shin JH et al. J Biol Chem 2003 Dec 05;278(49):49053-62

PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.

Locovei AM et al. Cell Div 2006 Nov 17;1:27

PMID:10683155 - A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.

Griffiths D et al. J Cell Sci 2000 Mar;113 ( Pt 6):1075-88

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:9366552 - A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast.

Adachi Y et al. Genes Cells 1997 Jul;2(7):467-79

PMID:9383050 - Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.

Forsburg SL et al. Genetics 1997 Nov;147(3):1025-41

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

PMID:7957098 - Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.

Saka Y et al. EMBO J 1994 Nov 15;13(22):5319-29

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:11523776 - Sna41goa1, a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with polalpha.

Uchiyama M et al. Mol Genet Genomics 2001 Aug;265(6):1039-49

PMID:20799962 - A genetic screen for replication initiation defective (rid) mutants in Schizosaccharomyces pombe.

Locovei AM et al. Cell Div 2010 Aug 27;5:20

PMID:6943408 - Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.

Nasmyth K et al. Mol Gen Genet 1981;182(1):119-24

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:22279046 - Rif1 is a global regulator of timing of replication origin firing in fission yeast.

Hayano M et al. Genes Dev 2012 Jan 15;26(2):137-50

PMID:34608864 - DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.

Aricthota S et al. Elife 2021 Oct 05;10

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:15388803 - Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.

Spiga MG et al. Nucleic Acids Res 2004;32(16):4945-53

PMID:11359920 - Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.

Takeda T et al. Mol Biol Cell 2001 May;12(5):1257-74

PMID:10747035 - Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18.

Kearsey SE et al. EMBO J 2000 Apr 03;19(7):1681-90

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:8895665 - The ORC1 homolog orp1 in fission yeast plays a key role in regulating onset of S phase.

Grallert B et al. Genes Dev 1996 Oct 15;10(20):2644-54

PMID:11136247 - Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures.

Lee JK et al. Proc Natl Acad Sci U S A 2001 Jan 02;98(1):54-9

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:21971174 - Abundance of prereplicative complexes (Pre-RCs) facilitates recombinational repair under replication stress in fission yeast.

Maki K et al. J Biol Chem 2011 Dec 02;286(48):41701-41710

PMID:9658174 - Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex.

Sherman DA et al. Mol Biol Cell 1998 Jul;9(7):1833-45

PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.

Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.

Hart EA et al. Curr Genet 2002 Aug;41(5):342-8

PMID:11532929 - Expression of Cdc18/Cdc6 and Cdt1 during G2 phase induces initiation of DNA replication.

Yanow SK et al. EMBO J 2001 Sep 03;20(17):4648-56

PMID:9914167 - Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay.

Liang DT et al. J Cell Sci 1999 Feb;112 ( Pt 4):559-67

PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

Kanke M et al. EMBO J 2012 May 02;31(9):2182-94

PMID:19228417 - MCM-GINS and MCM-MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast.

Akman G et al. BMC Cell Biol 2009 Feb 19;10:12

PMID:18180284 - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.

Bailis JM et al. Mol Cell Biol 2008 Mar;28(5):1724-38

PMID:10766248 - The Cdt1 protein is required to license DNA for replication in fission yeast.

Nishitani H et al. Nature 2000 Apr 06;404(6778):625-8

PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.

Li JJ et al. FEBS Lett 2011 Dec 15;585(24):3850-5

PMID:16899242 - Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.

Namdar M et al. Exp Cell Res 2006 Oct 15;312(17):3360-9

PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.

Gómez EB et al. Genetics 2008 Jun;179(2):757-71

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:21945095 - Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1).

Taylor M et al. DNA Repair (Amst) 2011 Nov 10;10(11):1154-63

GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:11027263 - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.

Snaith HA et al. Mol Cell Biol 2000 Nov;20(21):7922-32

PMID:28481910 - PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

Daigaku Y et al. PLoS Genet 2017 May;13(5):e1006789

PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.

Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:7876346 - The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.

Forsburg SL et al. J Cell Sci 1994 Oct;107 ( Pt 10):2779-88

PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.

Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9

PMID:35567482 - Determinants of RPA megafoci localization to the nuclear periphery in response to replication stress.

Kim SM et al. G3 (Bethesda) 2022 Jul 06;12(7)

PMID:9154809 - A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis.

Uchiyama M et al. Mol Cell Biol 1997 Jun;17(6):3103-15

PMID:9679144 - Regulation of cell polarity by microtubules in fission yeast.

Sawin KE et al. J Cell Biol 1998 Jul 27;142(2):457-71

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:18753627 - Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks.

Nitani N et al. Proc Natl Acad Sci U S A 2008 Sep 02;105(35):12973-8

PMID:21593208 - CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.

Fukuura M et al. Mol Biol Cell 2011 Jul 15;22(14):2620-33

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:10725227 - A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.

Grallert B et al. J Cell Sci 2000 Apr;113 ( Pt 8):1447-58

PMID:23045396 - Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

Sabatinos SA et al. Mol Cell Biol 2012 Dec;32(24):4986-97

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.

Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16

PMID:10588642 - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

Pasion SG et al. Mol Biol Cell 1999 Dec;10(12):4043-57

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.

Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48

PMID:11854402 - Essential role of MCM proteins in premeiotic DNA replication.

Lindner K et al. Mol Biol Cell 2002 Feb;13(2):435-44

PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.

Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:10770926 - Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.

Lee JK et al. J Biol Chem 2000 Jun 23;275(25):18871-8

GO_REF:0000002 - Comments

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.

Liang DT et al. Genetics 2001 Oct;159(2):471-86

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:8631307 - Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle.

Maiorano D et al. EMBO J 1996 Feb 15;15(4):861-72

PMID:1454522 - Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication.

Coxon A et al. Nucleic Acids Res 1992 Nov 11;20(21):5571-7

PMID:21813639 - Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.

Ding L et al. J Biol Chem 2011 Sep 23;286(38):32918-30

PMID:11973289 - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.

Gómez EB et al. Genetics 2002 Apr;160(4):1305-18

PMID:15338237 - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.

Dolan WP et al. Chromosoma 2004 Sep;113(3):145-56

PMID:12419251 - Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication.

Wuarin J et al. Cell 2002 Nov 01;111(3):419-31

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:16829518 - The replicative helicases of bacteria, archaea, and eukarya can unwind RNA-DNA hybrid substrates.

Shin JH et al. J Biol Chem 2006 Sep 15;281(37):26914-21