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protein coding gene - pus3 (SPCC16C4.06c) - tRNA pseudouridine synthase Pus3

Gene summary

Standard name
pus3
Systematic ID
SPCC16C4.06c
Product
tRNA pseudouridine synthase Pus3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74451
ORFeome ID
22/22E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 668412..671333 reverse strand

Annotation

PBO:0004474 - 4.2.1.70

Disease association

MONDO:0024553 - myopathy, lactic acidosis, and sideroblastic anemia 1

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GO biological process

GO:1990481 - mRNA pseudouridine synthesis

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GO:0031119 - tRNA pseudouridine synthesis

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005634 - nucleus

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GO molecular function

GO:0003723 - RNA binding

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GO:0106029 - tRNA pseudouridine synthase activity

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01416PseudoU_synth_1PsdUridine_synth_TruA_a/b_domPfam
cd02568PseudoU_synth_PUS1_PUS2PUS1/PUS2-likeCDD
G3DSA:3.30.70.580:FF:000035CATH-FunFam
G3DSA:3.30.70.580TruA/RsuA/RluB/E/F_NCATH-Gene3D
G3DSA:3.30.70.660PsdUridine_synth_TruA_CCATH-Gene3D
SSF55120PsdUridine_synth_cat_dom_sfSUPERFAMILY
PTHR11142PsdUridine_synth_TruAPANTHER
TIGR00071hisT_truAPsdUridine_synth_TruANCBIFAM

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:40829803 - MoDorado: enhanced detection of tRNA modifications in nanopore sequencing by off-label use of modification callers.
Rübsam FNM et al. Nucleic Acids Res 2025 Aug 11;53(15)
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12